Results 81 to 90 of about 4,481,323 (329)

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Fluorescence-based protocol for revealing cellular arrangement in biofilms

STAR Protocols, 2023
Summary: Standardized assays have greatly advanced the understanding of multicellular bacterial biofilms, but they lack cell-scale detail. Here, we present a fluorescence-based protocol that builds on past assays to reveal the cellular-scale arrangement ...
Devina Puri, Xin Fang, Kyle R. Allison
doaj  

Cell Detection by Functional Inverse Diffusion and Non-negative Group Sparsity$-$Part II: Proximal Optimization and Performance Evaluation

, 2018
In this two-part paper, we present a novel framework and methodology to analyze data from certain image-based biochemical assays, e.g., ELISPOT and Fluorospot assays. In this second part, we focus on our algorithmic contributions. We provide an algorithm
Jaldén, Joakim, Pla, Pol del Aguila
core   +1 more source

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays

Marine Drugs, 2014
The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of ...
Laia Reverté, Lucía Soliño, Olga Carnicer, J. Diogène, M. Campàs   +4 more
semanticscholar   +1 more source

Cell transformation assays for prediction of carcinogenic potential: State of the science and future research needs [PDF]

, 2011
Copyright @ 2011 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use ...
A. D. Scott, A. Poth, A. Sakai, Asada, Ashby, Bernardi, Berwald, Berwald, Boehm, Campisi, Carmichael, Cohen, Dolma, Evan, F. L. Martin, Farmer, H. Yasaei, Haga, Huberman, Isfort, Isfort, Isfort, J. S. Harvey, K. Pant, K. Sasaki, Kelly, Kerckaert, Kirkland, Kirkland, L. M. Schechtman, Layer, LeBoeuf, LeBoeuf, M. J. Aardema, M. R. O'Donovan, Martin, Martinez-Lopez de Castro, Mauthe, McCarvill, N. Tanaka, Newbold, Newbold, P. L. Carmichael, Pang, Pant, Pant, Pfuhler, R. F. Newbold, R. R. Shen, Robinson, Russo, S. Creton, Sakai, Sakai, Schechtman, Schinzel, Shen, Sheng, Stampfer, Stinghen, Syed, Trevisan, Visvader, Walsh, Zakut, Zhao   +65 more
core   +2 more sources

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

On-chip ultrasonic sample preparation for cell based assays†

, 2015
We demonstrate an acoustophoresis method for size-based separation, isolation, up-concentration and trapping of cells that can be used for on-chip sample preparation combined with high resolution imaging for cell-based assays.
I. Iranmanesh, H. Ramachandraiah, A. Russom, M. Wiklund   +3 more
semanticscholar   +1 more source

Active Gel Model of Amoeboid Cell Motility

, 2013
We develop a model of amoeboid cell motility based on active gel theory. Modeling the motile apparatus of a eukaryotic cell as a confined layer of finite length of poroelastic active gel permeated by a solvent, we first show that, due to active stress ...
Callan-Jones, A. C., Voituriez, R.
core   +1 more source

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source