Results 31 to 40 of about 1,066,214 (229)

Network analysis of hepatocellular carcinoma liquid biopsies augmented by single-cell sequencing data

Frontiers in Genetics, 2022
Liquid biopsy, the analysis of body fluids, represents a promising approach for disease diagnosis and prognosis with minimal intervention. Sequencing cell-free RNA derived from liquid biopsies has been very promising for the diagnosis of several diseases.
Aram Safrastyan, Aram Safrastyan, Damian Wollny, Damian Wollny, Damian Wollny   +4 more
doaj   +1 more source

Evaluation of circulating cell-free nucleic acids in plasma as biomarkers of laryngeal cancer [PDF]

Cellular, Molecular and Biomedical Reports
It has been aimed to compare the level of cell-free nucleic acids (DNA, RNA, and miRNA) concentrations in laryngeal cancer patients with the control group composed of healthy individuals. It has been utilized 46 patients with laryngeal carcinoma who were
Basak Yavuz, Ozge Cil, Akin Cayir
doaj   +1 more source

Biomarker potential of the GRP78 cell-free RNA in endometrial cancer

Egyptian Journal of Medical Human Genetics, 2022
Background Circulating tumor cells represent an opportunity for the assessment of early recurrent disease or for real-time tracing of cancer. Glucose Regulated Protein 78 (GRP78) is known in the literature as a stress factor in endometrial cancer.
Busra Aynekin, Hilal Akalin, I. Ipek Muderris, Gokhan Acmaz, Hulya Akgun, Izem Olcay Şahin, Nuriye Coşkun Gokce, Zahraa Alzaidi, Gözde Erturk Zararsiz, Yusuf Ozkul, Munis Dundar, Çetin Saatci   +11 more
doaj   +1 more source

A comprehensive evaluation of full-spectrum cell-free RNAs highlights cell-free RNA fragments for early-stage hepatocellular carcinoma detection

eBioMedicine, 2023
Various studies have reported cell-free RNAs (cfRNAs) as noninvasive biomarkers for detecting hepatocellular carcinoma (HCC). However, they have not been independently validated, and some results are contradictory. We provided a comprehensive evaluation of various types of cfRNA biomarkers and a full mining of the biomarker potential of new features of
Ning, Chun, Cai, Peng, Liu, Xiaofan, Li, Guangtao, Bao, Pengfei, Yan, Lu, Ning, Meng, Tang, Kaichen, Luo, Yi, Guo, Hua, Wang, Yunjiu, Wang, Zhuoran, Chen, Lu, Lu, Zhi John, Yin, Jianhua   +14 more
openaire   +2 more sources

Circulating RNA biomarkers in diffuse large B-cell lymphoma: a systematic review

Experimental Hematology & Oncology, 2021
Diffuse large B-cell lymphoma (DLBCL) is the most common histological subtype of non-Hodgkin’s lymphomas (NHL). DLBCL is an aggressive malignancy that displays a great heterogeneity in terms of morphology, genetics and biological behavior.
Philippe Decruyenaere, Fritz Offner, Jo Vandesompele   +2 more
doaj   +1 more source

Integrated analysis of circulating cell free nucleic acids for cancer genotyping and immune phenotyping of tumor microenvironment

Frontiers in Genetics, 2023
The circulating cell-free nucleic acids (ccfNAs) consist of a heterogenous cocktail of both single (ssNA) and double-stranded (dsNA) nucleic acids. These ccfNAs are secreted into the blood circulation by both healthy and malignant cells via various ...
Muskan Chaddha, Hemlata Rai, Ritu Gupta, Deepshi Thakral   +3 more
doaj   +1 more source

Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts

Frontiers in Bioengineering and Biotechnology, 2021
Cell-free gene expression systems have emerged as a promising platform for field-deployed biosensing and diagnostics. When combined with programmable toehold switch-based RNA sensors, these systems can be used to detect arbitrary RNAs and freeze-dried ...
Anibal Arce, Anibal Arce, Anibal Arce, Fernando Guzman Chavez, Chiara Gandini, Juan Puig, Juan Puig, Tamara Matute, Tamara Matute, Jim Haseloff, Neil Dalchau, Jenny Molloy, Keith Pardee, Fernán Federici, Fernán Federici, Fernán Federici, Fernán Federici   +16 more
doaj   +1 more source

Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

Nature Communications, 2020
Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal ...
Mingxia Zhang, Yuan Zou, Xing Xu, Xuebing Zhang, Mingxuan Gao, Jia Song, Peifeng Huang, Qin Chen, Zhi Zhu, Wei Lin, Richard N. Zare, Chaoyong Yang   +11 more
doaj   +1 more source

Liquid biopsy by analysis of circulating myeloma cells and cell-free nucleic acids: a novel noninvasive approach of disease evaluation in multiple myeloma

Biomarker Research, 2023
Multiple myeloma (MM) is an incurable hematological cancer with high spatial- and temporal-heterogeneity. Invasive single-point bone marrow sampling cannot capture the tumor heterogeneity and is difficult to repeat for serial assessments.
Shuchan Li, Enfan Zhang, Zhen Cai
doaj   +1 more source

Polyadenylation ligation‐mediated sequencing (PALM‐Seq) characterizes cell‐free coding and non‐coding RNAs in human biofluids

Clinical and Translational Medicine, 2022
Background Cell‐free messenger RNA (cf‐mRNA) and long non‐coding RNA (cf‐lncRNA) are becoming increasingly important in liquid biopsy by providing biomarkers for disease prediction, diagnosis and prognosis, but the simultaneous characterization of coding
Zhongzhen Liu, Taifu Wang, Xi Yang, Qing Zhou, Sujun Zhu, Juan Zeng, Haixiao Chen, Jinghua Sun, Liqiang Li, Jinjin Xu, Chunyu Geng, Xun Xu, Jian Wang, Huanming Yang, Shida Zhu, Fang Chen, Wen‐Jing Wang   +16 more
doaj   +1 more source