Results 21 to 30 of about 1,066,214 (229)

Cell-Free RNA Transcriptome and Prediction of Adverse Pregnancy Outcomes. [PDF]

Clin Chem, 2022
Gray KJ, Hemberg M, Karumanchi SA.
europepmc   +3 more sources

Cell-free translation of murine coronavirus RNA [PDF]

Journal of Virology, 1982
The coding assignments of the intracellular murine hepatitis virus-specific subgenomic RNA species and murine hepatitis virion RNA have been investigated by cell-free translation. The six murine hepatitis virus-specific subgenomic RNAs were partially purified by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate,
J L, Leibowitz, S R, Weiss, E, Paavola, C W, Bond   +3 more
openaire   +2 more sources

The amniotic fluid cell-free transcriptome in spontaneous preterm labor

Scientific Reports, 2021
The amniotic fluid (AF) cell-free RNA was shown to reflect physiological and pathological processes in pregnancy, but its value in the prediction of spontaneous preterm delivery is unknown.
Gaurav Bhatti, Roberto Romero, Nardhy Gomez-Lopez, Roger Pique-Regi, Percy Pacora, Eunjung Jung, Lami Yeo, Chaur-Dong Hsu, Mahendra Kavdia, Adi L. Tarca   +9 more
doaj   +1 more source

Cell free translation of chicken calcitonin messenger RNA [PDF]

FEBS Letters, 1983
The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)‐rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine.
Lasmoles, F., Desplan, C., Segond, N., Jullienne, A., Treilhou-Lahille, F., Milhaud, G., Moukhtar, M.S.   +6 more
openaire   +2 more sources

Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Human Genomics, 2022
Background Blood plasma, one of the most studied liquid biopsies, contains various molecules that have biomarker potential for cancer detection, including cell-free DNA (cfDNA) and cell-free RNA (cfRNA). As the vast majority of cell-free nucleic acids in
Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, Jo Vandesompele   +10 more
doaj   +1 more source

Cell-free RNA as a Fetal Timekeeper [PDF]

Clinical Chemistry, 2018
Cancer and fetal development share a lot. This is nothing new. The 19th century pathologist John Beard noted the similarities between a tumor and a trophoblast. Building on that, Lloyd Old, a pioneer in cancer immunology said, “If you've ever seen the trophoblast invading the uterus, it invades, spreads, creates a blood supply.
openaire   +2 more sources

Plasma cell-free RNA characteristics in COVID-19 patients [PDF]

Genome Research, 2022
The pathogenesis of COVID-19 is still elusive, which impedes disease progression prediction, differential diagnosis, and targeted therapy. Plasma cell-free RNAs (cfRNAs) carry unique information from human tissue and thus could point to resourceful solutions for pathogenesis and host-pathogen interactions.
Yanqun Wang, Jie Li, Lu Zhang, Hai-Xi Sun, Zhaoyong Zhang, Jinjin Xu, Yonghao Xu, Yu Lin, Airu Zhu, Yuxue Luo, Haibo Zhou, Yan Wu, Shanwen Lin, Yuzhe Sun, Fei Xiao, Ruiying Chen, Liyan Wen, Wei Chen, Fang Li, Rijing Ou, Yanjun Zhang, Tingyou Kuo, Yuming Li, Lingguo Li, Jing Sun, Kun Sun, Zhen Zhuang, Haorong Lu, Zhao Chen, Guoqiang Mai, Jianfen Zhuo, Puyi Qian, Jiayu Chen, Huanming Yang, Jian Wang, Xun Xu, Nanshan Zhong, Jingxian Zhao, Junhua Li, Jincun Zhao, Xin Jin   +40 more
openaire   +2 more sources

Cell-Free Translation of Paramyxovirus Messenger RNA [PDF]

Journal of Virology, 1973
Polypeptides corresponding in electrophoretic mobility to virion polypeptides 1, 3, and 5 were made in a reticulocyte cell-free system to which 18 S RNA from Sendai virus-infected cells was added. Immune precipitation was used to select relevant polypeptides from endogenous products.
openaire   +2 more sources

Exploring the cell-free total RNA transcriptome in diffuse large B-cell lymphoma and primary mediastinal B-cell lymphoma patients as biomarker source in blood plasma liquid biopsies

Frontiers in Oncology, 2023
IntroductionDiffuse large B-cell lymphoma (DLBCL) and primary mediastinal B-cell lymphoma (PMBCL) are aggressive histological subtypes of non-Hodgkin’s lymphoma.
Philippe Decruyenaere, Philippe Decruyenaere, Philippe Decruyenaere, Edoardo Giuili, Edoardo Giuili, Kimberly Verniers, Kimberly Verniers, Jasper Anckaert, Jasper Anckaert, Katrien De Grove, Malaïka Van der Linden, Dries Deeren, Jo Van Dorpe, Fritz Offner, Jo Vandesompele, Jo Vandesompele   +15 more
doaj   +1 more source

An in vitro Assay of mRNA 3’ end Using the E. coli Cell-free Expression System

Bio-Protocol, 2022
At the end of about 80% of the operon in Escherichia coli, translation termination decouples transcription, leading to Rho-dependent transcription termination (RDT).
Monford Paul Abishek N, Heon Lim
doaj   +1 more source