Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy [PDF]
Cells, 2021 Propagation of paternal sperm-contributed mitochondrial genes, resulting in heteroplasmy, is seldom observed in mammals due to post-fertilization degradation of sperm mitochondria, referred to as sperm mitophagy.
Won-Hee Song +6 more
doaj +2 more sources
Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability.
Jiaojiao Li +5 more
doaj +2 more sources
O2-Tuned Protein Synthesis Machinery in Escherichia coli-Based Cell-Free System [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Involved in most aerobic biochemical processes, oxygen affects cellular functions, and organism behaviors. Protein synthesis, as the underlying biological process, is unavoidably affected by the regulation of oxygen delivery and utilization.
Xiaomei Lin +5 more
doaj +2 more sources
A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha [PDF]
Biology, 2022 Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim of the
Nawal Abd El-Baky +5 more
doaj +2 more sources
Cell-Free PURE System: Evolution and Achievements
BioDesign Research, 2022 The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an in vitro open environment without a complete living cell.
Yi Cui, Xinjie Chen, Ze Wang, Yuan Lu
doaj +3 more sources
A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins [PDF]
BioengineeringCell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivation ...
Jeffrey L. Schloßhauer +3 more
doaj +2 more sources
Cell lysates and egg white create homeostatic microenvironment for gene expression in cell-free system [PDF]
Synthetic and Systems Biotechnology, 2018 Homeostasis widely exists in living systems, and plays essential roles for maintaining normal physiological activities, enabling to preserve their functionalities against variations.
Yang Liu, Dayong Yang
doaj +2 more sources
Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses [PDF]
Scientific Reports, 2017 Membrane proteins are key elements in cell-mediated processes. In particular, G protein-coupled receptors (GPCRs) have attracted increasing interest since they affect cellular signaling.
Anne Zemella +5 more
doaj +2 more sources
OnePot PURE Cell-Free System [PDF]
Journal of Visualized Experiments, 2021 The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In comparison, a home-made approach can be customized based on user needs.
Grasemann, Laura +3 more
openaire +2 more sources
Translation initiation consistency between in vivo and in vitro bacterial protein expression systems
Frontiers in Bioengineering and Biotechnology, 2023 Strict on-demand control of protein synthesis is a crucial aspect of synthetic biology. The 5′-terminal untranslated region (5′-UTR) is an essential bacterial genetic element that can be designed for the regulation of translation initiation.
Jiaojiao Li +10 more
doaj +1 more source