Rapid cell-free forward engineering of novel genetic ring oscillators [PDF]
eLife, 2015 While complex dynamic biological networks control gene expression in all living organisms, the forward engineering of comparable synthetic networks remains challenging. The current paradigm of characterizing synthetic networks in cells results in lengthy
Henrike Niederholtmeyer +6 more
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Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals [PDF]
The Ukrainian Biochemical Journal, 2020 The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was ...
A. K. Gulevskyy +2 more
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An empirical energy landscape reveals mechanism of proteasome in polypeptide translocation
eLife, 2022 The ring-like ATPase complexes in the AAA+ family perform diverse cellular functions that require coordination between the conformational transitions of their individual ATPase subunits (Erzberger and Berger, 2006; Puchades et al., 2020).
Rui Fang +3 more
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Frontiers in Bioengineering and Biotechnology, 2022 Kinetic modeling has relied on using a tedious number of mathematical equations to describe molecular kinetics in interacting reactions. The long list of differential equations with associated abstract variables and parameters inevitably hinders readers’
Yijie Deng +5 more
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Improved cell-free RNA and protein synthesis system. [PDF]
PLoS ONE, 2014 Cell-free RNA and protein synthesis (CFPS) is becoming increasingly used for protein production as yields increase and costs decrease. Advances in reconstituted CFPS systems such as the Protein synthesis Using Recombinant Elements (PURE) system offer new
Jun Li +3 more
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Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system. [PDF]
, 2018 Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell.
Joseph, Simpson +4 more
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Ubiquitous Cell-Free Massive MIMO Communications [PDF]
, 2019 Since the first cellular networks were trialled in the 1970s, we have witnessed an incredible wireless revolution. From 1G to 4G, the massive traffic growth has been managed by a combination of wider bandwidths, refined radio interfaces, and network ...
Björnson, Emil +4 more
core +2 more sources
Supramolecular protein assembly in cell-free protein synthesis system
Bioresources and Bioprocessing, 2022 Protein-based biomaterials have the characteristics of stability and biocompatibility. Based on these advantages, various bionic materials have been manufactured and used in different fields.
Zhixia Li +6 more
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Validation of a blood plasma separation system by biomarker detection [PDF]
, 2010 A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood.
Campbell, Colin J. +4 more
core +1 more source
Akaby-Cell-free protein expression system for linear templates.
PLoS ONE, 2022 Cell-free protein expression is increasingly becoming popular for biotechnology, biomedical and research applications. Among cell-free systems, the most popular one is based on Escherichia coli (E. coli). Endogenous nucleases in E.
Wakana Sato +6 more
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