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Cell-free systems for biotransformation

The cell-free systems based biotransformation has significantly transformed the field of biotechnology by facilitating the synthesis of therapeutic agents, biofuels and other commercially valuable chemicals. The current chapter initially focuses on presenting how the biotransformation process has conveniently transformed form the conventional cell-free
Huzaifa, Ibrahim   +5 more
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Cell-Free Massive MIMO systems

2015 49th Asilomar Conference on Signals, Systems and Computers, 2015
Cell-Free Massive MIMO systems comprise a large number of distributed, low cost, and low power access point antennas, connected to a network controller. The number of antennas is significantly larger than the number of users. The system is not partitioned into cells and each user is served by all access point antennas simultaneously.
Elina Nayebi   +3 more
openaire   +1 more source

Cell free systems for biodesign

Cell-free systems (CFS) have emerged as transformative tools in synthetic biology, enabling the execution of complex biological reactions such as transcription and translation outside the confines of living cells. By eliminating the cellular membrane, CFS allows unprecedented control over biochemical conditions, facilitating rapid prototyping (up to ...
Mohd, Tariq   +6 more
openaire   +2 more sources

Enterovirus eclipse in a cell-free system

Virology, 1964
Abstract When purified P32-labeled ECHO 7 virus is centrifuged at high speed, a RNase-susceptible P32-labeled compound is released. The interaction of purified but not centrifuged virus and erythrocyte receptor substance was studied. Irreversible attachment to soluble and purified receptor from human erythrocyte membranes appears to be enzymatic ...
L, PHILIPSON, M, LIND
openaire   +2 more sources

Computational biology for cell-free systems

Cell-free systems (CFS) decouple gene expression and metabolic pathways from living cells, offering a rapid, modular platform for biosensing, pathway prototyping, and protein production. This review surveys mechanistic and data-driven computational approaches tailored to CFS design and optimization.
Mansi, Acharya, Indra, Mani
openaire   +2 more sources

Systems for the Cell-Free Synthesis of Proteins

2011
We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given ...
Lei, Kai   +8 more
openaire   +2 more sources

In silico assessment of cell‐free systems

Biotechnology and Bioengineering, 2012
AbstractCell‐free extract (CFX)‐derived biocatalytic systems are usually embedded in a complex metabolic network, which makes chemical insulation of the production system necessary by removing enzymatic connections. While insulation can be performed by different methods, the identification of potentially disturbing reactions can become a rather lengthy
Bujara M, Panke S
openaire   +3 more sources

Cell-free systems for vaccine production

Cell-free (CF) systems is harness cellular components including tRNAs, ribosomes, and polymerase to synthesize proteins in vitro. Owing to their significant CF systems offer substantial advantages over traditional cell-based systems, including higher speed, biosafety, and portability.
Nhat, Le Bui   +4 more
openaire   +2 more sources

Cell-free systems for automation and robotics

The amalgamation of cell-free systems with automation and robotics is transforming the domains of biotechnology and bioengineering. This chapter examines the swiftly advancing domain of robotic platforms utilizing cell-free protein synthesis (CFPS) and other programmable cell-free biological mechanisms.
Juveriya, Israr   +2 more
openaire   +2 more sources

Cell-free systems for nanobiomaterials assembly

Nanobiomaterials-engineered constructs operating at the 1-100 nm scale-integrate biological macromolecules such as nucleic acids and proteins with synthetic polymers or inorganic nanocomponents to achieve programmable functionality in biomedical and industrial contexts.
Anjana Eettikkal, Mathew   +1 more
openaire   +2 more sources

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