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Cell-free systems for biotransformation
The cell-free systems based biotransformation has significantly transformed the field of biotechnology by facilitating the synthesis of therapeutic agents, biofuels and other commercially valuable chemicals. The current chapter initially focuses on presenting how the biotransformation process has conveniently transformed form the conventional cell-freeHuzaifa, Ibrahim +5 more
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Cell-Free Massive MIMO systems
2015 49th Asilomar Conference on Signals, Systems and Computers, 2015Cell-Free Massive MIMO systems comprise a large number of distributed, low cost, and low power access point antennas, connected to a network controller. The number of antennas is significantly larger than the number of users. The system is not partitioned into cells and each user is served by all access point antennas simultaneously.
Elina Nayebi +3 more
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Cell free systems for biodesign
Cell-free systems (CFS) have emerged as transformative tools in synthetic biology, enabling the execution of complex biological reactions such as transcription and translation outside the confines of living cells. By eliminating the cellular membrane, CFS allows unprecedented control over biochemical conditions, facilitating rapid prototyping (up to ...Mohd, Tariq +6 more
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Enterovirus eclipse in a cell-free system
Virology, 1964Abstract When purified P32-labeled ECHO 7 virus is centrifuged at high speed, a RNase-susceptible P32-labeled compound is released. The interaction of purified but not centrifuged virus and erythrocyte receptor substance was studied. Irreversible attachment to soluble and purified receptor from human erythrocyte membranes appears to be enzymatic ...
L, PHILIPSON, M, LIND
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Computational biology for cell-free systems
Cell-free systems (CFS) decouple gene expression and metabolic pathways from living cells, offering a rapid, modular platform for biosensing, pathway prototyping, and protein production. This review surveys mechanistic and data-driven computational approaches tailored to CFS design and optimization.Mansi, Acharya, Indra, Mani
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Systems for the Cell-Free Synthesis of Proteins
2011We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given ...
Lei, Kai +8 more
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In silico assessment of cell‐free systems
Biotechnology and Bioengineering, 2012AbstractCell‐free extract (CFX)‐derived biocatalytic systems are usually embedded in a complex metabolic network, which makes chemical insulation of the production system necessary by removing enzymatic connections. While insulation can be performed by different methods, the identification of potentially disturbing reactions can become a rather lengthy
Bujara M, Panke S
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Cell-free systems for vaccine production
Cell-free (CF) systems is harness cellular components including tRNAs, ribosomes, and polymerase to synthesize proteins in vitro. Owing to their significant CF systems offer substantial advantages over traditional cell-based systems, including higher speed, biosafety, and portability.Nhat, Le Bui +4 more
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Cell-free systems for automation and robotics
The amalgamation of cell-free systems with automation and robotics is transforming the domains of biotechnology and bioengineering. This chapter examines the swiftly advancing domain of robotic platforms utilizing cell-free protein synthesis (CFPS) and other programmable cell-free biological mechanisms.Juveriya, Israr +2 more
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Cell-free systems for nanobiomaterials assembly
Nanobiomaterials-engineered constructs operating at the 1-100 nm scale-integrate biological macromolecules such as nucleic acids and proteins with synthetic polymers or inorganic nanocomponents to achieve programmable functionality in biomedical and industrial contexts.Anjana Eettikkal, Mathew +1 more
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