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Cell-Free Noncanonical Redox Cofactor Systems
2022Noncanonical redox cofactor systems utilize nicotinamide adenine dinucleotide (phosphate), NAD(P)H, mimics to perform biotransformation reactions. Compared to systems utilizing native NAD(P)H, these noncanonical redox cofactors can offer decreased cost of cofactor supply, improved system activities, and can even supply reducing power directly to ...
William B, Black, Han, Li
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Cell-free protein synthesis systems
Biotechnology Advances, 1998Cell-free protein synthesis systems enable the direct in vitro expression of proteins from template DNA or RNA. Use of biochemical and bioengineering techniques has greatly improved the yields and productivities of cell-free systems. In some cases, the yields approach the in vivo levels.
H, Nakano, T, Yamane
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Cell-Free Artificial Photosynthesis System
2021The objective of this research is to create a cell-free artificial platform for harvesting light energy and transforming the energy to organic compounds. In order to achieve this objective, we took the approach of mimicking the photosynthetic processes of a plant leaf and integrating them into a compact system using microfabrication technology ...
Xiang Ren, Moses Noh, Jack Gongyao Zhou
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Leishmania cell-free protein expression system
Methods, 2011Cell-free protein expression is an important tool for a rapid production, engineering and labeling of recombinant proteins. However the complex protocols for preparation of eukaryotic cell-free protein expression systems result in high manufacturing costs and limit their utility.
Kovtun, Oleksiy +5 more
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Cell-Free Optogenetic Gene Expression System
ACS Synthetic Biology, 2018Optogenetic tools provide a new and efficient way to dynamically program gene expression with unmatched spatiotemporal precision. To date, their vast potential remains untapped in the field of cell-free synthetic biology, largely due to the lack of simple and efficient light-switchable systems.
Premkumar Jayaraman +5 more
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2006
A disrupted cell is capable of synthesizing protein from amino acids depending upon the template RNA. This discovery, half a century ago, set the stage for biochemical studies on the gene expression process occurring in cells. The cell-free translation system successfully revealed the function of a number of key molecules participating in the ...
Takuya Ueda +2 more
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A disrupted cell is capable of synthesizing protein from amino acids depending upon the template RNA. This discovery, half a century ago, set the stage for biochemical studies on the gene expression process occurring in cells. The cell-free translation system successfully revealed the function of a number of key molecules participating in the ...
Takuya Ueda +2 more
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Cell-Free Massive MIMO systems
2015 49th Asilomar Conference on Signals, Systems and Computers, 2015Cell-Free Massive MIMO systems comprise a large number of distributed, low cost, and low power access point antennas, connected to a network controller. The number of antennas is significantly larger than the number of users. The system is not partitioned into cells and each user is served by all access point antennas simultaneously.
Elina Nayebi +3 more
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Cell-free artificial photosynthesis system
2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2017We developed a cell-free artificial photosynthesis platform conducting both light and dark reactions for harvesting light energy and transforming the energy to organic compounds. To the best of our knowledge, such a device had not been reported so far. This system was able to harvest light energy and transform the energy to organic compounds, mimicking
Xiang Ren +5 more
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Development of an Insect Cell-Free System
Current Pharmaceutical Biotechnology, 2010Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out post-translational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system.
Toru, Ezure +7 more
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2002
I Introduction.- 1 Cell-Free Protein Synthesis.- II Improved Classical (Batch) Cell-Free Translation Systems.- 2 Improved Batch Translation System Based on E. coli Extract.- 3 Improved Composition and Energy Supply for Bacterial Batch Systems.- 4 PURE System: New Cell-Free Translation System Reconstructed with Purified Components.- 5 Direct Expression ...
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I Introduction.- 1 Cell-Free Protein Synthesis.- II Improved Classical (Batch) Cell-Free Translation Systems.- 2 Improved Batch Translation System Based on E. coli Extract.- 3 Improved Composition and Energy Supply for Bacterial Batch Systems.- 4 PURE System: New Cell-Free Translation System Reconstructed with Purified Components.- 5 Direct Expression ...
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