Results 71 to 80 of about 6,347,699 (399)

Programmable cell-free transcriptional switches for antibodies detection

, 2021
We report here the development of a cell-free in-vitro transcription system for the detection of specific target antibodies. The approach is based on the use of programmable antigen-conjugated DNA-based conformational switches that, upon binding to a ...
Aitor, Patiño Diaz, Simona, Ranallo, Sara, Bracaglia, Alessandro, Porchetta, Tania, Patiño, Francesco, Ricci   +5 more
core   +1 more source

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Nature Communications, 2018
The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics.
Thapakorn Jaroentomeechai, Jessica C. Stark, A. Natarajan, Cameron J. Glasscock, Laura E. Yates, Karen J. Hsu, M. Mrksich, M. Jewett, M. DeLisa   +8 more
semanticscholar   +1 more source

The Role of Hematopoietic Cell Transplantation in Ataxia‐Telangiectasia

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Ataxia‐telangiectasia (A‐T) is a DNA repair disorder characterized by neurodegeneration, immunodeficiency, and cancer predisposition. Hematopoietic cell transplantation (HCT) is an established therapy in related disorders such as Fanconi anemia (FA) and Nijmegen breakage syndrome (NBS), but its role in A‐T is unclear.
Laila Alkhouli, Seth Rotz, Masatoshi Takagi, Richa Sharma   +3 more
wiley   +1 more source

Label-free haemogram using wavelength modulated Raman spectroscopy for identifying immune-cell subset

, 2014
The authors thank the UK EPSRC the CR-UK/EPSRC/MRC/DoH (England) imaging Program and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding, K.D. is a Royal Society-Wolfson Merit Award Holder.Leucocytes in the blood of mammals form a
Bavishna B. Praveen, Powis, S.J., Campbell, E.C., Ashok, P.C., Praveen, B.B., Simon J. Powis, Dholakia, K., Praveen C. Ashok, Elaine C. Campbell, Kishan Dholakia   +9 more
core   +1 more source

Establishing a cell-free Vibrio natriegens expression system

bioRxiv, 2018
The fast growing bacterium Vibrio natriegens is an emerging microbial host for biotechnology. Harnessing its productive cellular components may offer a compelling platform for rapid protein production and prototyping of metabolic pathways or genetic ...
Daniel J. Wiegand, Henry H. Lee, N. Ostrov, G. Church   +3 more
semanticscholar   +1 more source

Effects of the Fluid Replacement Method During Online Hemodiafiltration on the Solute Removal Performance and Biocompatibility Using the Asymmetric Cellulose Triacetate Membrane

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Pre‐dilution online hemodiafiltration (Pre‐HDF) is predominantly used in Japan, whereas post‐dilution online HDF (Post‐HDF) is more common in Europe. An asymmetric cellulose triacetate (ATA) membrane may improve biocompatibility.
Kenji Sakurai, Yoshitaka Kurihara, Fumi Yamauchi, Hiromi Hosoya, Takeshi Saito   +4 more
wiley   +1 more source

Relationship between cyclic AMP production and lipolysis induced by forskolin in rat fat cells.

Journal of Lipid Research, 1992
Forskolin (7 beta-acetoxy-8, 13-epoxy-1 alpha,6 beta,9 alpha-trihydroxy-labd-14-ene-11-one) induced both cyclic AMP production and lipolysis in intact fat cells, but stimulated lipolysis without increasing cyclic AMP at a concentration of 10(-5) M ...
H Okuda, C Morimoto, T Tsujita
doaj   +1 more source

A novel, resistance-linked ovine PrP variant and its equivalent mouse variant modulate the in vitro cell-free conversion of rPrP to PrPres

, 2006
Prion diseases are associated with the conversion of the normal cellular prion protein, PrPC, to the abnormal, disease-associated form, PrPSc. This conversion can be mimicked in vitro by using a cell-free conversion assay. It has recently been shown that
Kirby, Louise, Manson, Jean C., Houston, Fiona, Goldmann, Wilfred, Gill, Andrew C.   +4 more
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source