Results 81 to 90 of about 6,347,699 (399)

Fatty acid synthesis in cell-free system from rabbit aorta

Journal of Lipid Research, 1966
The objectives of this study were to identify the subcellular fraction responsible for fatty acid synthesis in rabbit aorta and to determine the effect of cholesterol feeding on the system.
Arthur F. Whereat
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium

, 2023
Cell-free expression (CFE) systems are fundamental to reconstituting metabolic pathways in vitro toward the construction of a synthetic cell. Although an Escherichia coli-based CFE system is well-established, simpler model organisms are necessary to ...
John I. Glass, Katarzyna P. Adamala, Andrei Sakai (16031037), Hans A. Heus, Frank H. T. Nelissen (9460072), Katarzyna P. Adamala (6528353), Bob van Sluijs (4638499), Andrei Sakai, Aafke J. Jonker (16031040), Bob van Sluijs, Aafke J. Jonker, Hans A. Heus (344373), Frank H. T. Nelissen, Evan M. Kalb, Wilhelm T. S. Huck, Wilhelm T. S. Huck (8666409), Evan M. Kalb (16031043), John I. Glass (38230)   +17 more
core   +1 more source

A cell free system for functional centromere and kinetochore assembly Authors

Nature Protocols, 2012
This protocol describes a cell-free system for studying vertebrate centromere and kinetochore formation. We reconstitute tandem arrays of centromere protein A (CENP-A) nucleosomes as a substrate for centromere and kinetochore assembly.
Annika Guse, Colin J. Fuller, A. Straight   +2 more
semanticscholar   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

3D-Printed Bubble-Free Perfusion Cartridge System for Live-Cell Imaging

Sensors, 2020
The advent of 3D-printing technologies has had a significant effect on the development of medical and biological devices. Perfusion chambers are widely used for live-cell imaging in cell biology research; however, air-bubble invasion is a pervasive ...
Daigo Terutsuki, Hidefumi Mitsuno, Ryohei Kanzaki   +2 more
doaj   +1 more source

Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

BioTech, 2021
We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus ...
Marina Snapyan, Sylvain Robin, Garabet Yeretssian, Michèle Lecocq, Frédéric Marc, Vehary Sakanyan   +5 more
doaj   +1 more source

Downlink Cell-Free Fixed Wireless Access: Architectures, Physical Realities and Research Opportunities

, 2023
Recently a new paradigm of wireless access, termed as cell-free massive multiple-input multiple-output (MIMO), has drawn significant research interest.
Zheng, Gan, Minglei You, Rawi, Anas Al, Anas Al Rawi, Anvar Tukmanov, You, Minglei, Yangyishi Zhang, Tukmanov, Anvar, Zhang, Yangyishi, Gan Zheng   +9 more
core   +1 more source

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements.

Metabolic Engineering, 2016
Cell-free transcription-translation systems were originally applied towards in vitro protein production. More recently, synthetic biology is enabling these systems to be used within a systematic design context for prototyping DNA regulatory elements ...
R. Kelwick, A. Webb, J. T. MacDonald, P. Freemont   +3 more
semanticscholar   +1 more source