A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Only complete rejoining of DNA strand breaks after UVC allows K562 cell proliferation and DMSO induction of erythropoiesis [PDF]
, 2008 DNA strand breaks are early intermediates of the repair of UVC-induced DNA damage, however, since they severely impair cellular activities, their presence should be limited in time.
AVALLONE, BICE, FORESTI, MAGDA
core +1 more source
Three-Dimensional Cell Culture: A Breakthrough in Vivo
International Journal of Molecular Sciences, 2015 Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state.
D. Antoni +3 more
semanticscholar +1 more source
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
Cell Line Monolayer Tissue Culture FKDL dan Cell Line OL sebagai Media Penumbuh Virus pada Pengembangan Bioteknologi Biomolekuler (Penelitian Eksperimental Laboratoris) [PDF]
, 2012 Cell line sangatlah penting dalam dunia medis, khususnya dibidang diagnosis, produksi vaksin maupun dibidang bioteknologi serta biomolekuler. Berbagai penelitian telah dilakukan untuk tehnik pembuatan Cell Line Monolayer Tissue Culture.
Hasdianah, H. (Hasdianah)
core
FEBS Letters, EarlyView.Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello +6 more
wiley +1 more source
Transient down-regulation of beta1 integrin subtypes on kidney carcinoma cells is induced by mechanical contact with endothelial cell membranes [PDF]
, 2007 Adhesion molecules of the integrin beta1 family are thought to be involved in the malignant progression renal cell carcinoma (RCC). Still, it is not clear how they contribute to this process.
Achiwa H +10 more
core +1 more source
FEBS Letters, EarlyView.Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
Computational and Structural Biotechnology JournalThe secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental bottleneck in the manufacturing of protein-based therapeutics. Unconventional biological drugs with complex structures and processing requirements are particularly ...
Ying Bai +13 more
doaj +1 more source
The contribution of age structure to cell population responses to targeted therapeutics [PDF]
, 2012 Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner.
Ackleh +52 more
core +7 more sources