Automation of large scale transient protein expression in mammalian cells [PDF]
, 2011 Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Aricescu, Alexandru R +9 more
core +1 more source
Molecular Oncology, EarlyView.Cancer‐associated fibroblasts (CAFs) promote cancer growth, invasion (metastasis), and drug resistance. Here, we identified functional and diverse circulating CAFs (cCAFs) in patients with metastatic prostate cancer (mPCa). cCAFs were found in higher numbers and were functional and diverse in mPCa patients versus healthy individuals, suggesting their ...
Richell Booijink +6 more
wiley +1 more source
Novel design of biplanar electrodes in a multiwell plate for transepithelial electrical resistance measurement in 3D cell cultures [PDF]
Volume 387, 1 June 2025, 116413Recent advances in microphysiological systems have underscored the need for novel sensing and monitoring systems specifically designed for three-dimensional (3D) cell culture. In this article, an original architecture of a cell-culture multiwell plate embedding an impedance spectroscopy monitoring system is presented alongside a fast and ...
arxiv +1 more source
In vitro culture of the fastidious bacteria Candidatus Liberibacter asiaticus in association with insect feeder cells [PDF]
, 2011 Ca. Liberibacter asiaticus (LAS) is vectored by psyllids and is able to proliferate inside the insect. We therefore hypothesize that insect cells could act like feeder cells, providing nutrients in a continuous way and a favorable environment to the ...
Dollet, Michel +3 more
core
Leydig cells express neural cell adhesion molecules in vivo and in vitro [PDF]
, 1992 The neural cell adhesion molecule (NCAM) polypeptides are expressed by numerous tissues during embryonic development, where they are involved in cell-cell interactions.
Barthels, D. +7 more
core +1 more source
Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells
Molecular Oncology, EarlyView.In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi +3 more
wiley +1 more source
A chemotaxis-based explanation of spheroid formation in 3D cultures of breast cancer cells [PDF]
arXiv, 2018 Three-dimensional cultures of cells are gaining popularity as an in vitro improvement over 2D Petri dishes. In many such experiments, cells have been found to organize in aggregates. We present new results of three-dimensional in vitro cultures of breast cancer cells exhibiting patterns.
arxiv
Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion) [PDF]
, 2007 The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds.
Früh, Jennifer-Annika +5 more
core
Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry [PDF]
, 2009 Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular ...
Amit Shavit +2 more
core +2 more sources
A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer
Molecular Oncology, EarlyView.Lung cancer cells were spiked into donor blood to evaluate the recovery rates of the following circulating tumor cell (CTC) enrichment technologies: CellMag™, EasySep™, RosetteSep™, Parsortix® PR1, and Parsortix® Prototype systems. Each method's advantages and disadvantages are described.
Volga M Saini +11 more
wiley +1 more source