Results 21 to 30 of about 16,798,617 (404)

Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics*

open access: yesMolecular & Cellular Proteomics, 2002
Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and ...
Shao-En Ong   +6 more
semanticscholar   +1 more source

Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

open access: yesBMC Research Notes, 2018
Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi   +7 more
doaj   +1 more source

A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]

open access: yes, 2012
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson   +31 more
core   +2 more sources

Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.

open access: yesACS Biomaterials Science & Engineering, 2020
Advances in microscale 3D cell culture systems have helped to elucidate cellular physiology, understand mechanisms of stem cell differentiation, produce pathophysiological models, and reveal important cell-cell and cell-matrix interactions.
Sung-Min Kang   +3 more
semanticscholar   +1 more source

Cell Culture [PDF]

open access: yesCell, 2010
Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey.
  +5 more sources

Angiotensin II and Angiotensin Receptors 1 and 2—Multifunctional System in Cells Biology, What Do We Know?

open access: yesCells, 2021
For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja   +3 more
doaj   +1 more source

Engineered CHO cells as a novel AAV production platform for gene therapy delivery

open access: yesScientific Reports, 2023
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy   +7 more
doaj   +1 more source

Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]

open access: yes, 2018
An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula   +7 more
core   +1 more source

Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]

open access: yes, 2015
Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom   +2 more
core   +1 more source

Occurrence of spontaneous polyploids in Citrus

open access: yesHorticultural Science, 2006
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman   +3 more
doaj   +1 more source

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