Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology
BMC Research Notes, 2018 Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi +7 more
doaj +1 more source
A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
, 2012 In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson +31 more
core +2 more sources
Cells, 2021 For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja +3 more
doaj +1 more source
Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.
ACS Biomaterials Science & Engineering, 2020 Advances in microscale 3D cell culture systems have helped to elucidate cellular physiology, understand mechanisms of stem cell differentiation, produce pathophysiological models, and reveal important cell-cell and cell-matrix interactions.
Sung-Min Kang +3 more
semanticscholar +1 more source
Engineered CHO cells as a novel AAV production platform for gene therapy delivery
Scientific Reports, 2023 The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy +7 more
doaj +1 more source
Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion. [PDF]
, 1975 Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of
Armstrong, Peter, Lackie, J
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Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning
Frontiers in Pharmacology, 2018 Drug development is a lengthy and costly process that proceeds through several stages from target identification to lead discovery and optimization, preclinical validation and clinical trials culminating in approval for clinical use. An important step in
Sigrid A. Langhans
semanticscholar +1 more source
Occurrence of spontaneous polyploids in Citrus
Horticultural Science, 2006 Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman +3 more
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Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]
, 2018 An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula +7 more
core +1 more source
Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.
International Journal of Plant Biology, 2012 This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy +5 more
doaj +1 more source