Results 21 to 30 of about 15,168,841 (359)

Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application [PDF]

open access: yesPeerJ, 2017
Background Adipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported.
Fengli Zhang   +5 more
doaj   +2 more sources

T Cell Development in Culture [PDF]

open access: yesImmunity, 2002
The T cell compartment is continuously replenished by a renewable source of stem cells. In the adult, bone marrow-derived stem cells seed the thymus and initiate a developmental program that requires a series of incompletely understood signals that are normally provided by the thymus. Failure to recapitulate this process in simple in vitro cultures has
Michael J. Bevan, Sophie M. Lehar
openaire   +3 more sources

Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions

open access: yesCell & Bioscience, 2022
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects
Ciarra Almeria   +4 more
doaj   +1 more source

Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics*

open access: yesMolecular & Cellular Proteomics, 2002
Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and ...
Shao-En Ong   +6 more
semanticscholar   +1 more source

Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

open access: yesAMB Express, 2017
Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an
SooJin Han   +5 more
doaj   +1 more source

HIF-1α Is Associated with Resistance to Hypoxia-Induced Apoptosis in Ameloblastoma

open access: yesInternational Journal of Dentistry, 2021
Background. Ameloblastoma (AMB) is a benign odontogenic tumour, with an aggressive local behaviour and a high rate of recurrence. Previous studies have demonstrated that hypoxia-induced factor alpha 1 (HIF-1α) and activated caspase-3 contribute to tumour
Katherine Julissa Palma Valladares   +10 more
doaj   +1 more source

Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

open access: yesBMC Research Notes, 2018
Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi   +7 more
doaj   +1 more source

Angiotensin II and Angiotensin Receptors 1 and 2—Multifunctional System in Cells Biology, What Do We Know?

open access: yesCells, 2021
For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja   +3 more
doaj   +1 more source

Engineered CHO cells as a novel AAV production platform for gene therapy delivery

open access: yesScientific Reports, 2023
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy   +7 more
doaj   +1 more source

Occurrence of spontaneous polyploids in Citrus

open access: yesHorticultural Science, 2006
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman   +3 more
doaj   +1 more source

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