Results 31 to 40 of about 17,196,917 (410)

Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

open access: yesBMC Research Notes, 2018
Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO).
Saikant Regidi   +7 more
doaj   +1 more source

A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]

open access: yes, 2012
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson   +31 more
core   +2 more sources

Angiotensin II and Angiotensin Receptors 1 and 2—Multifunctional System in Cells Biology, What Do We Know?

open access: yesCells, 2021
For years, the renin-angiotensin system (RAS) has been perceived as a system whose role is to primarily modulate the functioning of the cardiovascular system.
Maksymilian Ziaja   +3 more
doaj   +1 more source

Engineered CHO cells as a novel AAV production platform for gene therapy delivery

open access: yesScientific Reports, 2023
The Herpes simplex virus (HSV)-based platform for production of recombinant adeno-associated viral vectors (rAAVs) yields higher titers and increased percentage of full capsids when compared to the triple transient transfection (TTT) method.
Abdou Nagy   +7 more
doaj   +1 more source

Molluscan cells in culture: primary cell cultures and cell lines [PDF]

open access: yesCanadian Journal of Zoology, 2013
In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models.
T P, Yoshino, U, Bickham, C J, Bayne
openaire   +2 more sources

Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]

open access: yes, 2018
An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula   +7 more
core   +1 more source

Cell culture forensics [PDF]

open access: yesProceedings of the National Academy of Sciences, 2001
Genomics, proteomics, vaccinology, transgenics, stem cell—advances in all these areas critically stack on the shoulders of tissue culture, our ability to cultivate an organism's living cells in plastic dishes. Nutritional trial and error for decades of painstaking cell gardening laid the groundwork for the several thousand human primary cell explants ...
openaire   +2 more sources

Occurrence of spontaneous polyploids in Citrus

open access: yesHorticultural Science, 2006
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman   +3 more
doaj   +1 more source

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

open access: yesFrontiers in Pharmacology, 2018
Drug development is a lengthy and costly process that proceeds through several stages from target identification to lead discovery and optimization, preclinical validation and clinical trials culminating in approval for clinical use. An important step in
Sigrid A. Langhans
semanticscholar   +1 more source

Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.

open access: yesInternational Journal of Plant Biology, 2012
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy   +5 more
doaj   +1 more source

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