A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
, 2012 In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson +31 more
core +2 more sources
Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells
Cells, 2021 Mesenchymal stem cells (MSCs) are of great interest for their use in cell-based therapies due to their multipotent differentiation and immunomodulatory capacities.
Ilias Nikolits +4 more
doaj +1 more source
Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]
, 2020 The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Aldave, Anthony J +14 more
core
In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines
Czech Journal of Food Sciences, 2019 In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity.
Cetin Akca +5 more
doaj +1 more source
Development and cytotoxic response of two proliferative MDA- MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines [PDF]
, 2017 Triple-Negative Basal-Like tumors, representing 15 to 20% of breast cancers, are very aggressive and with poor prognosis. Targeted therapies have been developed extensively in preclinical and clinical studies to open the way for new treatment strategies.
arxiv +1 more source
Metabolic Regulatory Network Kinetic Modeling with Multiple Isotopic Tracers for iPSCs [PDF]
arXiv, 2023 The rapidly expanding market for regenerative medicines and cell therapies highlights the need to advance the understanding of cellular metabolisms and improve the prediction of cultivation production process for human induced pluripotent stem cells (iPSCs). In this paper, a metabolic kinetic model was developed to characterize underlying mechanisms of
arxiv
Co-culture of Adult Mesenchymal Stem Cells and Nucleus Pulposus Cells in Bilaminar Pellets for Intervertebral Disc Regeneration [PDF]
, 2009 Background: Our goal is to optimize stem cell-based tissue engineering strategies in the context of the intervertebral disc environment. We explored the benefits of co-culturing nucleus pulposus cells (NPC) and adult mesenchymal stem cells (MSC) using a ...
Allon, AA, Lotz, JC, Schneider, RA
core +2 more sources
Frontiers in Cell and Developmental Biology, 2020 The introduction of advanced therapy medicinal products (ATMPs) to the global pharma market has been revolutionizing the pharmaceutical industry and has opened new routes for treating various types of cancers and incurable diseases.
Roya Ramezankhani +21 more
doaj +1 more source
A low-cost, user-friendly rheo-optical compression assay to measure mechanical properties of cell spheroids in standard cell culture plates [PDF]
arXiv, 2023 The mechanical characterization of cell spheroids, one of the most widely used 3D biology models in vitro, is a hotspot of current research on the role played by the mechanical response of cells and tissues. The techniques proposed so far in the literature, while providing important scientific insights, require a specialized equipment and technical ...
arxiv
Single cell measurement of telomerase expression and splicing using microfluidic emulsion cultures. [PDF]
, 2014 Telomerase is a reverse transcriptase that maintains telomeres on the ends of chromosomes, allowing rapidly dividing cells to proliferate while avoiding senescence and apoptosis.
Hart, Kristina +2 more
core +1 more source