Results 31 to 40 of about 15,168,841 (359)

Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

open access: yesMicrobial Cell Factories, 2022
Background Escherichia coli (E. coli) is a promising host for production of recombinant proteins (including antibodies and antibody fragments) that don’t require complex post-translational modifications such as glycosylation.
Tomasz K. Baginski   +8 more
doaj   +1 more source

Modeling Physiological Events in 2D vs. 3D Cell Culture.

open access: yesPhysiology, 2017
Cell culture has become an indispensable tool to help uncover fundamental biophysical and biomolecular mechanisms by which cells assemble into tissues and organs, how these tissues function, and how that function becomes disrupted in disease.
Kayla E A Duval   +6 more
semanticscholar   +1 more source

Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.

open access: yesInternational Journal of Plant Biology, 2012
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy   +5 more
doaj   +1 more source

Comparative Antimicrobial Effects of Rat Blood-derived Products and Adipose-derived Mesenchymal Stem Cell Conditioned Medium [PDF]

open access: yesResearch in Molecular Medicine, 2023
Background: The antimicrobial characteristics of biological products are fundamental in medicine due to their ability to treat microbial infections. This research aimed to assess and compare the antimicrobial properties of various platelet concentrates ...
Mohsen Sheykhhasan   +3 more
doaj  

Global Perspective of Novel Therapeutic Strategies for the Management of NeuroAIDS

open access: yesBiomolecular Concepts, 2018
Among Human immunodeficiency virus (HIV) infected individuals, around two-thirds of patients present with neuroAIDS, where HIV-associated neurocognitive disorders (HAND), and HIV-associated dementia (HAD) are the most prevailing neurological ...
Kumar Swatantra   +4 more
doaj   +1 more source

Response of letter to the editor on Procalcitonin: a promising diagnostic marker for sepsis and antibiotic therapy

open access: yesJournal of Intensive Care, 2017
In a letter to the editor, Raineri SM et al. have given an insight of another dimension of procalcitonin (PCT) use as a diagnostic tool in invasive candidiasis.
R. Saikant   +6 more
doaj   +1 more source

Microsystems for Cell Cultures

open access: yesBiosensors, 2022
Microfabricated systems are increasingly being utilized in biotechnological, biomedical, and pharmaceutical research and development as replacements for traditional in vitro cell cultures, bioreactors, and animal experiments (Figure 1) [...]
openaire   +3 more sources

Cell isolation and culture [PDF]

open access: yesWormBook, 2013
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C.
Sihui Zhang, Jeffrey R. Kuhn
openaire   +3 more sources

Optimized exosome isolation protocol for cell culture supernatant and human plasma

open access: yesJournal of Extracellular Vesicles, 2015
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of ...
R. Lobb   +6 more
semanticscholar   +1 more source

Cell Culture [PDF]

open access: yesCell, 2010
Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey.
openaire   +3 more sources

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