Results 71 to 80 of about 17,196,917 (410)
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Absorption and Tensility of Bioactive Sutures Prepared for Cell Transplantation [PDF]
, 2013 Biodegradable scaffolds are widely used to transplant stem cells into various tissues. Recent studies showed that living stem cells can be attached to the surface of absorbable sutures in vitro.
Horváthy, Dénes +6 more
core +2 more sources
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Dorsal and ventral stimuli in cell–material interactions: effect on cell morphology [PDF]
, 2012 Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of more relevant culture conditions,
Curtis ASG +9 more
core +2 more sources
3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications
Frontiers in Cardiovascular Medicine, 2019 Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs.
C. Zuppinger
semanticscholar +1 more source
Hematopoietic (stem) cells—The elixir of life?
FEBS Letters, EarlyView.The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo +4 more
wiley +1 more source
Review of 3D Cell Culture with Analysis in Microfluidic Systems.
Analytical Methods, 2019 A review with 105 references that analyzes the emerging research area of 3D cell culture in microfluidic platforms with integrated detection schemes. Over the last several decades a central focus of cell culture has been the development of better in vivo
Andre D. Castiaux, D. Spence, R. Martin
semanticscholar +1 more source
FEBS Letters, EarlyView.Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
In vitro culture of the fastidious bacteria Candidatus Liberibacter asiaticus in association with insect feeder cells [PDF]
, 2011 Ca. Liberibacter asiaticus (LAS) is vectored by psyllids and is able to proliferate inside the insect. We therefore hypothesize that insect cells could act like feeder cells, providing nutrients in a continuous way and a favorable environment to the ...
Dollet, Michel +3 more
core
Bta-miR-10b secreted by bovine embryos negatively impacts preimplantation embryo quality [PDF]
, 2019 In a previous study, we found miR-10b to be more abundant in a conditioned culture medium of degenerate embryos compared to that of blastocysts. Here, we show that miR-10b mimics added to the culture medium can be taken up by embryos. This uptake results
Deforce, Dieter +6 more
core +1 more source