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Advanced Cell Culture Techniques for Cancer Drug Discovery [PDF]

open access: yesBiology, 2014
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment.
Carrie J. Lovitt   +2 more
doaj   +8 more sources

“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy [PDF]

open access: yesStem Cells International, 2011
Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose
Chandana Tekkatte   +3 more
doaj   +3 more sources

Biomimetic Scaffolds-A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering. [PDF]

open access: goldNanomaterials (Basel)
Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional
Górnicki T   +14 more
europepmc   +4 more sources

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

open access: yesFrontiers in Molecular Biosciences, 2020
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen   +5 more
doaj   +2 more sources

Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory

open access: yesJournal of Microbiology & Biology Education, 2017
Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as ...
Kristen Bowey-Dellinger   +12 more
doaj   +2 more sources

Cell Culture Techniques

open access: yesNeuromethods, 2011
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
Swati Rauthan
semanticscholar   +3 more sources

Liver cell culture techniques.

open access: yesMethods in molecular biology, 2009
Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
J. Castell, M. Gómez‐Lechón
semanticscholar   +5 more sources

Minireview: Culture of airway epithelial cells: Research techniques

open access: greenExperimental Lung Research, 1986
Techniques for culturing respiratory tract epithelium have evolved steadily over the last 30 years. Initial work focussed on the maintenance of sections of airway mucosa in organ culture using serum-supplemented medium. More recent advances have permitted the growth of dissociated airway epithelial cells in fully defined conditions for extended periods
Van R, Ruoh‐Fang Yen, Boucher R.C.
openalex   +5 more sources

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