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“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy [PDF]
Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose
Chandana Tekkatte+3 more
doaj +5 more sources
Is It Time to Start Transitioning From 2D to 3D Cell Culture?
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen+5 more
doaj +4 more sources
Advanced Cell Culture Techniques for Cancer Drug Discovery [PDF]
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment.
Carrie J. Lovitt+2 more
doaj +5 more sources
The contribution of age structure to cell population responses to targeted therapeutics [PDF]
Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position.
Ackleh+52 more
arxiv +8 more sources
Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens [PDF]
A monoclonal antibody was used to detect an early antigen of cytomegalovirus (CMV) by fluorescence 16 h after inoculation of MRC-5 monolayers in 1-dram (ca. 3.7-ml) shell vials and low-speed centrifugation.
C. Gleaves+3 more
semanticscholar +2 more sources
Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as ...
Kristen Bowey-Dellinger+12 more
doaj +2 more sources
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
Cristina Suñol+2 more
openaire +3 more sources
An introductory undergraduate course covering animal cell culture techniques [PDF]
Animal cell culture is a core laboratory technique in many molecular biology, developmental biology, and biotechnology laboratories. Cell culture is a relatively old technique that has been sparingly taught at the undergraduate level.
P. Mozdziak, J. Petitte, S. Carson
semanticscholar +2 more sources
Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
José V. Castell+1 more
openaire +5 more sources
Cell and Tissue Culture Technique [PDF]
Paul Geisert
openaire +3 more sources