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“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy [PDF]
Stem Cells International, 2011 Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose
Chandana Tekkatte +3 more
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Advanced Cell Culture Techniques for Cancer Drug Discovery [PDF]
Biology, 2014 Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment.
Carrie J. Lovitt +2 more
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Is It Time to Start Transitioning From 2D to 3D Cell Culture?
Frontiers in Molecular Biosciences, 2020 Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen +5 more
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The contribution of age structure to cell population responses to targeted therapeutics [PDF]
Journal of Theoretical Biology, 2012, 311, pp.19-27, 2011 Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position.
Ackleh +52 more
arxiv +8 more sources
Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens [PDF]
Journal of Clinical Microbiology, 1985 A monoclonal antibody was used to detect an early antigen of cytomegalovirus (CMV) by fluorescence 16 h after inoculation of MRC-5 monolayers in 1-dram (ca. 3.7-ml) shell vials and low-speed centrifugation.
C. Gleaves +3 more
semanticscholar +2 more sources
Journal of Microbiology & Biology Education, 2017 Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as ...
Kristen Bowey-Dellinger +12 more
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Neuromethods, 2011 1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
Cristina Suñol +2 more
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An introductory undergraduate course covering animal cell culture techniques [PDF]
Biochemistry and Molecular Biology Education, 2004 Animal cell culture is a core laboratory technique in many molecular biology, developmental biology, and biotechnology laboratories. Cell culture is a relatively old technique that has been sparingly taught at the undergraduate level.
P. Mozdziak, J. Petitte, S. Carson
semanticscholar +2 more sources
Methods in molecular biology, 2009 Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
José V. Castell +1 more
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Cell and Tissue Culture Technique [PDF]
The American Biology Teacher, 1964 Paul Geisert
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