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Advanced Cell Culture Techniques for Cancer Drug Discovery [PDF]
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment.
Carrie J. Lovitt+2 more
doaj +8 more sources
“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy [PDF]
Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose
Chandana Tekkatte+3 more
doaj +3 more sources
Biomimetic Scaffolds-A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering. [PDF]
Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional
Górnicki T+14 more
europepmc +4 more sources
Is It Time to Start Transitioning From 2D to 3D Cell Culture?
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen+5 more
doaj +2 more sources
Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as ...
Kristen Bowey-Dellinger+12 more
doaj +2 more sources
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
Swati Rauthan
semanticscholar +3 more sources
Cell and Tissue Culture Technique [PDF]
Paul Geisert
openalex +3 more sources
Liver cell culture techniques.
Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
J. Castell, M. Gómez‐Lechón
semanticscholar +5 more sources
Minireview: Culture of airway epithelial cells: Research techniques
Techniques for culturing respiratory tract epithelium have evolved steadily over the last 30 years. Initial work focussed on the maintenance of sections of airway mucosa in organ culture using serum-supplemented medium. More recent advances have permitted the growth of dissociated airway epithelial cells in fully defined conditions for extended periods
Van R, Ruoh‐Fang Yen, Boucher R.C.
openalex +5 more sources
Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts. [PDF]
Smith LA+5 more
europepmc +3 more sources