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“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy [PDF]

Stem Cells International, 2011
Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose
Chandana Tekkatte, Gency Ponrose Gunasingh, K. M. Cherian, Kavitha Sankaranarayanan   +3 more
doaj   +7 more sources

Advanced Cell Culture Techniques for Cancer Drug Discovery [PDF]

Biology, 2014
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment.
Carrie J. Lovitt, Todd B. Shelper, Vicky M. Avery   +2 more
doaj   +7 more sources

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Frontiers in Molecular Biosciences, 2020
Cell culture is an important and necessary process in drug discovery, cancer research, as well as stem cell study. Most cells are currently cultured using two-dimensional (2D) methods but new and improved methods that implement three-dimensional (3D ...
Caleb Jensen, Caleb Jensen, Yong Teng, Yong Teng, Yong Teng, Yong Teng   +5 more
doaj   +4 more sources

Navigating stem cell culture: insights, techniques, challenges, and prospects [PDF]

Frontiers in Cell and Developmental Biology
Stem cell research holds huge promise for regenerative medicine and disease modeling, making the understanding and optimization of stem cell culture a critical aspect of advancing these therapeutic applications.
Aleksandra Górska, Mateusz Trubalski, Bartosz Borowski, Adam Brachet, Sylwia Szymańczyk, Renata Markiewicz   +5 more
doaj   +3 more sources

The contribution of age structure to cell population responses to targeted therapeutics [PDF]

Journal of Theoretical Biology, 2012, 311, pp.19-27, 2011
Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position.
Ackleh, Ackleh, Ainseba, Ainseba, Alarcón, Angulo, Angulo, Arino, Banks, Bekkal Brikci, Bekkal Brikci, Bernard, Calsina, Darren R. Tyson, Diekmann, Douglas, Doumic, Doumic, Dowling, Engel, Engl, Gabriel, Gabriel, Gerlee, Glenn F. Webb, Golubev, Gyllenberg, Gyllenberg, Gyllenberg, Hinow, Kostova, Lee, Liu, Mallet, Michel, Patel, Perthame, Perthame, Picart, Picart, Pierre Gabriel, Pilant, Pilant, Powathil, Quaranta, Quaranta, Ramis-Conde, Ribba, Rundell, Rundell, Shawn P. Garbett, Vito Quaranta, Webb   +52 more
arxiv   +8 more sources

Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens [PDF]

Journal of Clinical Microbiology, 1985
A monoclonal antibody was used to detect an early antigen of cytomegalovirus (CMV) by fluorescence 16 h after inoculation of MRC-5 monolayers in 1-dram (ca. 3.7-ml) shell vials and low-speed centrifugation.
C. Gleaves, T. F. Smith, E. Shuster, G. Pearson   +3 more
semanticscholar   +2 more sources

Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory

Journal of Microbiology & Biology Education, 2017
Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as ...
Kristen Bowey-Dellinger, Luke Dixon, Kristin Ackerman, Cynthia Vigueira, Yewseok K. Suh, Todd Lyda, Kelli Sapp, Michael Grider, Dinene Crater, Travis Russell, Michael Elias, V. McNeil Coffield, Verónica A. Segarra   +12 more
doaj   +2 more sources

Cell Culture Techniques

Neuromethods, 2011
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
Cristina Suñol, Anna Bal-Price, Michael Aschner   +2 more
openaire   +3 more sources

An introductory undergraduate course covering animal cell culture techniques [PDF]

Biochemistry and Molecular Biology Education, 2004
Animal cell culture is a core laboratory technique in many molecular biology, developmental biology, and biotechnology laboratories. Cell culture is a relatively old technique that has been sparingly taught at the undergraduate level.
P. Mozdziak, J. Petitte, S. Carson
semanticscholar   +2 more sources

Liver Cell Culture Techniques

Methods in molecular biology, 2009
Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
José V. Castell, María José Gómez-Lechón   +1 more
openaire   +5 more sources