Cytocentric measurement for regenerative medicine
Any Regenerative Medicine (RM) business requires reliably predictable cell and tissue products. Regulatory agencies expect control and documentation. However, laboratory tissue production is currently not predictable or well-controlled. Before conditions
Alicia D. Henn+11 more
doaj +1 more source
Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi+28 more
core +1 more source
An optimized method for isolating and expanding invariant natural killer T cells from mouse spleen [PDF]
The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells are therefore characterized as an innate T cell population capable of activating and steering ...
Drennan, Michael+2 more
core +1 more source
Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
Cell culture is one of the most valuable tools which is being applied in both fundamental and applied gastrointestinal research. The cells are isolated from their natural location (in vivo) and further propagated in vitro or artificial environment and ...
Gaur Preksha+2 more
doaj +1 more source
Automated Motion Analysis of Adherent Cells in Monolayer Culture [PDF]
This paper presents a novel method for tracking and characterizing adherent cells in monolayer culture. A system of cell tracking employing computer vision techniques was applied to time-lapse videos of replicate normal human uro-epithelial cell cultures
Bedder, M.+5 more
core +1 more source
Advanced imaging and tissue engineering of the human limbal epithelial stem cell niche [PDF]
The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors.
Daniels, JT+10 more
core +1 more source
Diel rhythmicity in amino acid uptake by Prochlorococcus [PDF]
The marine cyanobacterium Prochlorococcus, the most abundant phototrophic organism on Earth, numerically dominates the phytoplankton in nitrogen (N)-depleted oceanic gyres.
Burkill, Peter H.+7 more
core +3 more sources
Three-dimensional (3D) stem cell culture systems have attracted considerable attention as a way to better mimic the complex interactions between individual cells and the extracellular matrix (ECM) that occur in vivo.
Woochan Kim+4 more
doaj +1 more source
Design of thiol–ene photoclick hydrogels using facile techniques for cell culture applications
Thiol–ene photoclick hydrogels with tunable biomechanical and biochemical properties for biological applications, including controlled cell culture, regenerative medicine, and drug delivery.
L. Sawicki, April M. Kloxin
semanticscholar +1 more source
Optimized isolation and expansion of human airway epithelial basal cells from endobronchial biopsy samples [PDF]
Autologous airway epithelial cells have been used in clinical tissue-engineered airway transplantation procedures with a view to assisting mucosal regeneration and restoring mucociliary escalator function.
Birchall, MA+5 more
core +1 more source