Results 61 to 70 of about 1,147,382 (356)

Redox‐dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

FEBS Letters, EarlyView.
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker, Nimit Mistry, Nicoletta Plotegher, Elizabeth R. Westbrook, Michael D. E. Sewell, John Carroll, Gyorgy Szabadkai, Angus J. Bain, Michael R. Duchen   +8 more
wiley   +1 more source

Cartilage tissue formation from human adipose-derived stem cells via herbal component (Avocado/soybean unsaponifiables) in scaffold-free culture system

Dental Research Journal, 2020
Background: The use of stem cells, growth factors, and scaffolds to repair damaged tissues is a new idea in tissue engineering. The aim of the present study is the investigation of Avocado/soybean (A/S) effects on chondrogenic differentiation of human ...
Arefeh Basiri, Batool Hashemibeni, Mohammad Kazemi, Ali Valiani, Maryam Aliakbari, Nazem Ghasemi   +5 more
doaj   +1 more source

In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry [PDF]

Vojnosanitetski Pregled, 2019
Background/Aim. There is limited published evidence on the cytotoxicity of 3D printed polymer materials for dentistry applications, despite that they are now being widely used in medicine.
Puškar Tatjana, Trifković Branka, Đurović-Koprivica Daniela, Kojić Vesna, Jevremović Ana, Mirković Siniša, Eggbeer Dominic   +6 more
doaj   +1 more source

Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3 [PDF]

, 2016
Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course “design and optimization of biological systems” which is offered in the biochemistry curricula.
De Luca, Belén M., Gonzalez, Rodrigo Horacio, Nudel, Berta Clara, Nusblat, Alejandro David   +3 more
core   +1 more source

The cytochrome oxidase defect in ISC‐depleted yeast is caused by impaired iron–sulfur cluster maturation of the mitoribosome assembly factor Rsm22

FEBS Letters, EarlyView.
The yeast mitoribosome assembly factor Rsm22 contains a [4Fe‐4S] cluster that is matured by the mitochondrial iron–sulfur cluster assembly (ISC) machinery. Defects in ISC components result in impaired mitochondrial protein synthesis due to a mitoribosome assembly defect.
Ulrich Mühlenhoff, Dominik Trauth, Weronika Śliwińska, Linda Boss, Roland Lill   +4 more
wiley   +1 more source

An Extensive Method for Maintenance of Sterility in Mammalian Cell Culture Laboratory Routine

Challenges, 2017
Cell culture laboratories are designed to secure sterile environments for biological studies, and its susceptibility to biological contaminants is a major problem for researchers.
Selcen Çelik-Uzuner, Uğur Uzuner
doaj   +1 more source

The Alliance for Cellular Signaling Plasmid Collection: A Flexible Resource for Protein Localization Studies and Signaling Pathway Analysis [PDF]

, 2007
Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks.
Bryan, Heather, Chandy, Grischa, Chang, Mi Sook, Coutre, Sam, Dhandapani, Kavitha, Driver, Adrienne, Fraser, Iain D. C., Fung, Eileen, Gehrig, Elizabeth, Kivork, Christine, Lee, Genie, Liu, Jamie, Lyon, Stephen, Maer, Andreia, Meyer, Tobias, Mi, Qingli, Ning, Yuhong, O'Rourke, Nancy, Park, Wei Sun, Randhawa, Baljinder, Santat, Leah, Saunders, Brian, Simon, Melvin I., Subramaniam, Shankar, Verghese, Mary, Wall, Estelle, Whalen, James, Zavzavadjian, Joelle R., Zhu, Xiaocui   +28 more
core   +1 more source

Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. [PDF]

, 2019
Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases.
Aghaamoo, Mohammad, Digman, Michelle A, Kamalakshakurup, Gopakumar, Lee, Abraham P, Ma, Ning   +4 more
core   +1 more source

History of Cell Culture Techniques

The American Biology Teacher, 2010
Culturing Life: How Cells Became Technologies. By Hannah Landecker . 2007. Harvard University Press (ISBN 9780674034761). 276 pages. Paperback. $$18.95. Cell culture techniques have shaped the scope of biology since the start of the 20th century and are responsible for major advances in biomedicine.
openaire   +2 more sources

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source