High-throughput design of cultured tissue moulds using a biophysical model [PDF]
arXiv, 2023 The technique presented here identifies tethered mould designs, optimised for growing cultured tissue with very highly-aligned cells. It is based on a microscopic biophysical model for polarised cellular hydrogels. There is an unmet need for tools to assist mould and scaffold designs for the growth of cultured tissues with bespoke cell organisations ...
arxiv
Microelectrode arrays of diamond-insulated graphitic channels for real time detection of exocytotic events from cultured chromaffin cells and slices of adrenal glands [PDF]
Analytical Chemistry 88, 7493-7499 (2016), 2016 A microstructured graphitic 4x4 multielectrode array was embedded in a single crystal diamond substrate (4x4 {uG-SCD MEA) for real-time monitoring of exocytotic events from cultured chromaffin cells and adrenal slices. The current approach relies on the development of a parallel ion beam lithographic technique, which assures the time effective ...
arxiv +1 more source
An Overview of in vitro Methods to Study Microglia
Frontiers in Cellular Neuroscience, 2018 Neuroinflammation is a common feature in neurodegenerative diseases and strategies to modulate neuroinflammatory processes are increasingly considered as therapeutic options.
Raissa Timmerman +2 more
doaj +1 more source
Neurofilament phosphoforms: Surrogate markers for axonal injury, degeneration and loss [PDF]
, 2005 This review on the role of neurofilaments as surrogate markers for axonal degeneration in neurological diseases provides a brief background to protein synthesis, assembly, function and degeneration.
Petzold, A
core +1 more source
Modeling Physiological Events in 2D vs. 3D Cell Culture.
Physiology, 2017 Cell culture has become an indispensable tool to help uncover fundamental biophysical and biomolecular mechanisms by which cells assemble into tissues and organs, how these tissues function, and how that function becomes disrupted in disease.
Kayla E A Duval +6 more
semanticscholar +1 more source
Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review
International Journal of Molecular Sciences, 2020 Three-dimensional (3-D) cell culture models, such as spheroids, organoids, and organotypic cultures, are more physiologically representative of the human tumor microenvironment (TME) than traditional two-dimensional (2-D) cell culture models.
Ravi Teja Chitturi Suryaprakash +3 more
semanticscholar +1 more source
CARS and SHG microscopy to follow the collagen production in living human corneal fibroblasts and mesenchymal stem cells in fibrin gel 3D cultures [PDF]
, 2011 Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. CARS microscopy can detect lipid membranes and droplet compartments in living cells and SHG microscopy enables a strong imaging contrast for molecules ...
arxiv +1 more source
Inferring metabolic fluxes in nutrient-limited continuous cultures: A Maximum Entropy Approach with minimum information [PDF]
arXiv, 2021 We propose a new scheme to infer the metabolic fluxes of cell cultures in a chemostat. Our approach is based on the Maximum Entropy Principle and exploits the understanding of the chemostat dynamics and its connection with the actual metabolism of cells.
arxiv
Introducing monitoring and automation in cartilage tissue engineering, toward controlled clinical translation [PDF]
, 2011 The clinical application of tissue engineered products requires to be tightly connected with the possibility to control the process, assess graft quality and define suitable release criteria for implantation.
Santoro, Rosaria
core +1 more source
Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling
World Journal of Stem Cells, 2019 Three-dimensional (3D) culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.
Nipha Chaicharoenaudomrung +2 more
semanticscholar +1 more source