Results 31 to 40 of about 12,578,619 (347)

NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells

Bio-Protocol, 2017
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells.
Alvaro E. Galvis, Hugh Fisher, David Camerini   +2 more
doaj   +1 more source

Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

STAR Protocols, 2022
Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such
Cai Han, Linyu Sun, Qi Pan, Yumeng Sun, Wentao Wang, Yueqin Chen   +5 more
doaj   +1 more source

ALTERED FRACTIONATION RADIOTHERAPY FOR HEAD AND NECK CANCER

Žurnal Grodnenskogo Gosudarstvennogo Medicinskogo Universiteta, 2019
The purpose of the review is to analyze and summarize literature data about the role of altered fractionation radiotherapy for head and neck cancer. A total of 46 English-language literature sources were analyzed. They provide a radiobiological rationale
Parkhomenko L. B.
doaj   +1 more source

Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy

STAR Protocols, 2023
Summary: Here, we present a protocol to observe the three-dimensional surface of nuclear pore complexes (NPCs) of vertebrate early embryos by field emission scanning electron microscopy (FESEM).
Weimin Shen, Anming Meng
doaj   +1 more source

A method for the analysis of the oligomerization profile of the Huntington’s disease-associated, aggregation-prone mutant huntingtin protein by isopycnic ultracentrifugation

Frontiers in Molecular Biosciences
Conformational diseases, such as Alzheimer’s, Parkinson’s and Huntington’s diseases as well as ataxias and fronto-temporal disorders, are part of common class of neurological disorders characterised by the aggregation and progressive accumulation of ...
Raffaella Bonavita, Raffaella Bonavita, Rosaria Di Martino, Rosaria Di Martino, Giuseppe Cortone, Antonello Prodomo, Mariagrazia Di Gennaro, Mariagrazia Di Gennaro, Mariagrazia Di Gennaro, Gianluca Scerra, Valentino Panico, Silvia Nuzzo, Marco Salvatore, Sarah V. Williams, Fulvia Vitale, Maria Gabriella Caporaso, Massimo D’Agostino, Francesca M. Pisani, Angeleen Fleming, Maurizio Renna, Maurizio Renna   +20 more
doaj   +1 more source

Genetic Cell-Surface Modification for Optimized Foam Fractionation

Frontiers in Bioengineering and Biotechnology, 2020
Rhamnolipids are among the glycolipids that have been investigated intensively in the last decades, mostly produced by the facultative pathogen Pseudomonas aeruginosa using plant oils as carbon source and antifoam agent.
Christian C. Blesken, Isabel Bator, Isabel Bator, Christian Eberlein, Hermann J. Heipieper, Till Tiso, Till Tiso, Lars M. Blank, Lars M. Blank   +8 more
doaj   +1 more source

Comparison of peripheral leukocyte parameters in patients receiving conventionally and hypofractionated radiotherapy schemes for the treatment of newly diagnosed glioblastoma

Frontiers in Immunology, 2023
IntroductionTreatment for glioblastomas, aggressive and nearly uniformly fatal brain tumors, provide limited long-term success. Immunosuppression by myeloid cells in both the tumor microenvironment and systemic circulation are believed to contribute to ...
Lindsey Greenlund, Ryan Shanley, Ryan Shanley, Kellen Mulford, Elizabeth C. Neil, Elizabeth C. Neil, Jessica Lawrence, Jessica Lawrence, Susan Arnold, Susan Arnold, Michael Olin, Michael Olin, G. Elizabeth Pluhar, G. Elizabeth Pluhar, Andrew S. Venteicher, Andrew S. Venteicher, Clark C. Chen, Clara Ferreira, Margaret Reynolds, L. Chinsoo Cho, Christopher Wilke, Christopher Wilke, B. Aika Shoo, Jianling Yuan, Jianling Yuan, Kathryn Dusenbery, Kathryn Dusenbery, Lawrence R. Kleinberg, Stephanie A. Terezakis, Stephanie A. Terezakis, Lindsey Sloan, Lindsey Sloan   +31 more
doaj   +1 more source

Inferring structural variant cancer cell fraction [PDF]

Nature Communications, 2020
Abstract We present SVclone, a computational method for inferring the cancer cell fraction of structural variant (SV) breakpoints from whole-genome sequencing data. SVclone accurately determines the variant allele frequencies of both SV breakends, then simultaneously estimates the cancer cell fraction and SV copy number.
Marek Cmero, Ke Yuan, Cheng Soon Ong, Jan Schröder, David J. Adams, Pavana Anur, Rameen Beroukhim, Paul C. Boutros, David D. L. Bowtell, Peter J. Campbell, Shaolong Cao, Elizabeth L. Christie, Yupeng Cun, Kevin J. Dawson, Jonas Demeulemeester, Stefan C. Dentro, Amit G. Deshwar, Nilgun Donmez, Ruben M. Drews, Roland Eils, Yu Fan, Matthew W. Fittall, Dale W. Garsed, Moritz Gerstung, Gad Getz, Santiago Gonzalez, Gavin Ha, Kerstin Haase, Marcin Imielinski, Lara Jerman, Yuan Ji, Clemency Jolly, Kortine Kleinheinz, Juhee Lee, Henry Lee-Six, Ignaty Leshchiner, Dimitri Livitz, Salem Malikic, Iñigo Martincorena, Thomas J. Mitchell, Quaid D. Morris, Ville Mustonen, Layla Oesper, Martin Peifer, Myron Peto, Benjamin J. Raphael, Daniel Rosebrock, Yulia Rubanova, S. Cenk Sahinalp, Adriana Salcedo, Matthias Schlesner, Steven E. Schumacher, Subhajit Sengupta, Ruian Shi, Seung Jun Shin, Paul T. Spellman, Oliver Spiro, Lincoln D. Stein, Maxime Tarabichi, Peter Van Loo, Shankar Vembu, Ignacio Vázquez-García, Wenyi Wang, David C. Wedge, David A. Wheeler, Jeffrey A. Wintersinger, Tsun-Po Yang, Xiaotong Yao, Kaixian Yu, Hongtu Zhu, Niall M. Corcoran, Tony Papenfuss, Christopher M. Hovens, Florian Markowetz, Geoff Macintyre, Lauri A. Aaltonen, Federico Abascal, Adam Abeshouse, Hiroyuki Aburatani, David J. Adams, Nishant Agrawal, Keun Soo Ahn, Sung-Min Ahn, Hiroshi Aikata, Rehan Akbani, Kadir C. Akdemir, Hikmat Al-Ahmadie, Sultan T. Al-Sedairy, Fatima Al-Shahrour, Malik Alawi, Monique Albert, Kenneth Aldape, Ludmil B. Alexandrov, Adrian Ally, Kathryn Alsop, Eva G. Alvarez, Fernanda Amary, Samirkumar B. Amin, Brice Aminou, Ole Ammerpohl, Matthew J. Anderson, Yeng Ang, Davide Antonello, Pavana Anur, Samuel Aparicio, Elizabeth L. Appelbaum, Yasuhito Arai, Axel Aretz, Koji Arihiro, Shun-ichi Ariizumi, Joshua Armenia, Laurent Arnould, Sylvia Asa, Yassen Assenov, Gurnit Atwal, Sietse Aukema, J. Todd Auman, Miriam R. R. Aure, Philip Awadalla, Marta Aymerich, Gary D. Bader, Adrian Baez-Ortega, Matthew H. Bailey, Peter J. Bailey, Miruna Balasundaram, Saianand Balu, Pratiti Bandopadhayay, Rosamonde E. Banks, Stefano Barbi, Andrew P. Barbour, Jonathan Barenboim, Jill Barnholtz-Sloan, Hugh Barr, Elisabet Barrera, John Bartlett, Javier Bartolome, Claudio Bassi, Oliver F. Bathe, Daniel Baumhoer, Prashant Bavi, Stephen B. Baylin, Wojciech Bazant, Duncan Beardsmore, Timothy A. Beck, Sam Behjati, Andreas Behren, Beifang Niu, Cindy Bell, Sergi Beltran, Christopher Benz, Andrew Berchuck, Anke K. Bergmann, Erik N. Bergstrom, Benjamin P. Berman, Daniel M. Berney, Stephan H. Bernhart, Rameen Beroukhim, Mario Berrios, Samantha Bersani, Johanna Bertl, Miguel Betancourt, Vinayak Bhandari, Shriram G. Bhosle, Andrew V. Biankin, Matthias Bieg, Darell Bigner, Hans Binder, Ewan Birney, Michael Birrer, Nidhan K. Biswas, Bodil Bjerkehagen, Tom Bodenheimer, Lori Boice, Giada Bonizzato, Johann S. De Bono, Arnoud Boot, Moiz S. Bootwalla, Ake Borg, Arndt Borkhardt, Keith A. Boroevich, Ivan Borozan, Christoph Borst, Marcus Bosenberg, Mattia Bosio, Jacqueline Boultwood, Guillaume Bourque, Paul C. Boutros, G. Steven Bova, David T. Bowen, Reanne Bowlby, David D. L. Bowtell, Sandrine Boyault, Rich Boyce, Jeffrey Boyd, Alvis Brazma, Paul Brennan, Daniel S. Brewer, Arie B. Brinkman, Robert G. Bristow, Russell R. Broaddus, Benedikt Brors, Ivana Cataldo, Ken Chen, Vincenzo Corbo, Rebecca A. Dagg, Angelo P. Dei Tos, Xavier Estivill, Matteo Fassan, J. Lynn Fink, Milana Frenkel-Morgenstern, Anthony J. Gill, Sonia Grimaldi, Sean M. Grimmond, Seong Gu Heo, Austin J. Hepperla, Peter Hersey, Taobo Hu, Nigel B. Jamieson, Rory Johnson, Rosa Karlić, Karin S. Kassahn, Hiroto Katoh, Marat D. Kazanov, Catherine J. Kennedy, Loretta M. S. Lau, Rita T. Lawlor, Xiaobo Li, Laurence B. Lovat, Yong-Jie Lu, Luca Malcovati, Graham J. Mann, Erik Mayer, John D. McPherson, Neil D. Merrett, Linda Mileshkin, Lisa Mirabello, Arcadi Navarro, David E. Neal, Katia Nones, Hidenori Ojima, Choon Kiat Ong, Chawalit Pairojkul, Qiang Pan-Hammarström, Stefan M. Pfister, Tirso Pons, Raquel Rabionet, Markus Ringnér, Lewis R. Roberts, Michael H. A. Roehrl, Francisco Sanchez-Vega, Aldo Scarpa, Catherine A. Shang, Shimin Shuai, Johan Staaf, Michael R. Stratton, Tatsuhiko Tsunoda, Richard Turkington, Naveen Vasudev, Jiayin Wang, Anne Y. Warren, Justin P. Whalley, Kui Wu, Sergei Yakneen, Chen Ye, Yingyan Yu   +264 more
openaire   +10 more sources

Stable Isotope Fractionation of Metals and Metalloids in Plants: A Review

Frontiers in Plant Science, 2022
This work critically reviews stable isotope fractionation of essential (B, Mg, K, Ca, Fe, Ni, Cu, Zn, Mo), beneficial (Si), and non-essential (Cd, Tl) metals and metalloids in plants.
Matthias Wiggenhauser, Rebekah E. T. Moore, Peng Wang, Gerd Patrick Bienert, Kristian Holst Laursen, Simon Blotevogel   +5 more
doaj   +1 more source

Exploring the mechanisms of antigen processing by cell fractionation.

Current Opinion in Immunology, 1998
It is becoming increasingly clear that most of the intracellular compartments that contain MHC class II products in antigen-presenting cells simply represent the conventional endosomes and lysosomes that are expressed in all cell types.
P. Pierre, I. Mellman
semanticscholar   +1 more source