Results 51 to 60 of about 12,578,619 (347)

Protocol for mitochondrial isolation and sub-cellular localization assay for mitochondrial proteins

STAR Protocols, 2023
Summary: Here, we provide a protocol to isolate mitochondria from cultured cells and extract differently located mitochondrial proteins. We detail steps to separate both integral and peripheral membrane proteins from soluble proteins using sonication. We
Danyi Zhou, Sheng Zhong, Xinyu Han, Dandan Liu, Hezhi Fang, Ya Wang   +5 more
doaj   +1 more source

Biogenesis of the polymeric IgA receptor in rat hepatocytes. II. Localization of its intracellular forms by cell fractionation studies

Journal of Cell Biology, 1985
In the companion paper (Sztul, E. S., K. E. Howell, and G. E. Palade, J. Cell Biol., 100:1248-1254), we have shown that pulse labeling of hepatic proteins with [35S]cysteine can be obtained in vivo in intact rats.
E. Sztul, K. Howell, G. Palade
semanticscholar   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Method for estimation of apoptotic cell fraction of cytotherapy using in vivo fluorine-19 magnetic resonance: pilot study in a patient with head and neck carcinoma receiving tumor-infiltrating lymphocytes labeled with perfluorocarbon nanoemulsion [PDF]

, 2023
Eric T. Ahrens, Brooke Helfer, Charles F. O’Hanlon, Deanne Lister, Julie L Bykowski, Karen Messer, Benjamin I. Leach, Jiawen Chen, Hongyan Xu, Gregory A. Daniels, Ezra E.W. Cohen   +10 more
openalex   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Single‐Cell Nucleus Extraction with Cellular Indexing

Advanced Science
Bulk organelle‑fractionation masks cell‑to‑cell heterogeneity, and existing microfluidic methods cannot reliably reconnect each isolated organelle to its parent cell, an essential capability for multiomics readouts.
Trinh Lam, Ana Esmeralda Gomez Martinez, Alison Su, Anna Fomitcheva‐Khartchenko, Xin Wang, Md Nazibul Islam, Paul Lum, Amy Elizabeth Herr   +7 more
doaj   +1 more source

Experimental setup for the ultrasonic fractionation of flowing whole blood in a capillary

Current Directions in Biomedical Engineering, 2022
If a long ultrasound driving pulse is chosen such, that its frequency is greater than the resonance frequency of one cell type and less than the resonance frequency of another cell type, the cells are separated in different nodes and antinodes of the ...
Smalberger Charl, Nathan Malavika, Rubin David M., Nel Marietha, Kotopoulis Spiros, Carlson Craig S., Postema Michiel   +6 more
doaj   +1 more source

Single-cell isolation from full-thickness human intestinal tissue resections for single-cell RNA sequencing

STAR Protocols, 2023
Summary: Single-cell isolation techniques allow the investigation of physical and functional relationships between individual cells within a complex cell population.
Gail A. West, Shuai Zhao, Quang Tam Nguyen, Stephen M. Christensen, Ilyssa O. Gordon, Stefan D. Holubar, Kellie M. Kravarik, Claudio Fiocchi, Pranab K. Mukherjee, Florian Rieder   +9 more
doaj   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source