Experimental substantiation of permeabilized hepatocytes model for investigation of mitochondria in situ respiration [PDF]
The Ukrainian Biochemical Journal, 2015 To verify experimentally the model of permeabilized hepatocytes, the degree of cell permeability was assessed using trypan blue and polarographycally determined cell respiration rate upon succinate (0.35 mM) and α-ketoglutarate (1 mM) oxidation ...
V. M. Merlavsky +3 more
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Role of Second Quinone Binding Site in Proton Pumping by Respiratory Complex I
Frontiers in Chemistry, 2019 Respiratory complex I performs the reduction of quinone (Q) to quinol (QH2) and pumps protons across the membrane. Structural data on complex I have provided spectacular insights into the electron and proton transfer paths, as well as into the long (~30 ...
Outi Haapanen +3 more
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Біологічні студії, 2023 Background. Assessment of cell viability is crucial in cell studies. Testing plasma membrane integrity is a traditional approach of evaluating cell viability. Mitochondrial functional capacity closely correlates with plasma membrane integrity and overall
Anastasiia Zub +4 more
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Largen: A Molecular Regulator of Mammalian Cell Size Control [PDF]
, 2014 Little is known about how mammalian cells maintain cell size homeostasis. We conducted a novel genetic screen to identify cell-size-controlling genes and isolated Largen, the product of a gene (PRR16) that increased cell size upon overexpression in human
Gandin, Valentina +22 more
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Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy. [PDF]
, 2021 BACKGROUND Because of the interplay between mitochondrial respiration and cellular metabolism, the simultaneous monitoring of both cellular processes provides important insights for the understanding of biological processes. NMR flow systems provide a
Hertig, Damian +17 more
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Nrf2 impacts cellular bioenergetics by controlling substrate availability for mitochondrial respiration [PDF]
, 2013 Transcription factor Nrf2 and its repressor Keap1 regulate a network of cytoprotective genes involving more than 1% of the genome, their best known targets being drug-metabolizing and antioxidant genes.
Iain Hargreaves +29 more
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ESTIMATING CONTRIBUTION OF ZOOXANTHELLAE TO ANIMAL RESPIRATION (CZAR) AND TO ANIMAL GROWTH (CZAG) OF GIANT CLAM Tridacna maxima [PDF]
, 2006 Zooxanthellae are symbiotic dinoflagellate algae which live in association with marine invertebrates including giant clams. These algae are capable of translocating part of their photosynthetic products to the host.
Ambariyanto, A
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Sensors, 2012 Sensors and multi-sensor arrays are the basis of new technologies for the non-label monitoring of cell activity. In this paper we show that choroid plexus cells can be cultured on silicon chips and that sensors register in real time changes in their ...
Soledad García Gómez de las Heras +2 more
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Cell-Based Sensor System Using L6 Cells for Broad Band Continuous Pollutant Monitoring in Aquatic Environments [PDF]
, 2012 Pollution of drinking water sources represents a continuously emerging problem in global environmental protection. Novel techniques for real-time monitoring of water quality, capable of the detection of unanticipated toxic and bioactive substances, are ...
Ulrich Bohrn +7 more
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ND3 Cys39 in complex I is exposed during mitochondrial respiration [PDF]
, 2022 Mammalian complex I can adopt catalytically active (A-) or deactive (D-) states. A defining feature of the reversible transition between these two defined states is thought to be exposure of the ND3 subunit Cys39 residue in the D-state and its occlusion ...
Burger, Nils +41 more
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