Results 41 to 50 of about 827,991 (338)

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

Rapid, label‐free enrichment of lymphocytes in a closed system using a flow‐through microfluidic device

Bioengineering & Translational Medicine
The majority of adoptive cellular therapies are produced from peripheral mononuclear cells obtained via leukapheresis and further enriched for the cells of interest (e.g., T cells).
Anton Mukhamedshin, Riley C. Reddington, Mai T. P. Dinh, Kumar Abhishek, Mubasher Iqbal, Marc Manheim, Sean C. Gifford, Sergey S. Shevkoplyas   +7 more
doaj   +1 more source

Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines, 2020
Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery.
Rohollah Nasiri, Amir Shamloo, Javad Akbari, Peyton Tebon, Mehmet R. Dokmeci, Samad Ahadian   +5 more
doaj   +1 more source

Ablation of LRP6 in alpha‐smooth muscle actin‐expressing cells abrogates lung inflammation and fibrosis upon bleomycin‐induced lung injury

FEBS Letters, EarlyView.
Low‐density lipoprotein receptor‐related protein 6 (LRP6) is a key receptor for the Wnt antagonist Dickkopf1 (DKK1). DKK1 protein expression is induced in a bleomycin (BLM)‐induced lung injury model. We show that DKK1 induces proinflammatory and profibrotic genes in lung fibroblasts.
Eun‐Ah Sung, Mikhail G. Dozmorov, SuJeong Song, Theingi Aung, Min Hee Park, Patricia J. Sime, Wook‐Jin Chae   +6 more
wiley   +1 more source

The PIP Peptide of INFLORESCENCE DEFICIENT IN ABSCISSION Enhances Populus Leaf and Elaeis guineensis Fruit Abscission

Plants, 2019
The programmed loss of a plant organ is called abscission, which is an important cell separation process that occurs with different organs throughout the life of a plant.
Timothy John Tranbarger, Hubert Domonhédo, Michel Cazemajor, Carole Dubreuil, Urs Fischer, Fabienne Morcillo   +5 more
doaj   +1 more source

Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases

FEBS Letters, EarlyView.
The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher, Paula Armina V. Buco, Jia Q. Truong, Paul A. Ramsland, Monique F. Smeets, Carl R. Walkley, Jessica K. Holien   +6 more
wiley   +1 more source

Acoustofluidic separation of cells and particles

Microsystems & Nanoengineering, 2019
AbstractAcoustofluidics, the integration of acoustics and microfluidics, is a rapidly growing research field that is addressing challenges in biology, medicine, chemistry, engineering, and physics. In particular, acoustofluidic separation of biological targets from complex fluids has proven to be a powerful tool due to the label-free, biocompatible ...
Mengxi Wu, Adem Ozcelik, Joseph Rufo, Zeyu Wang, Rui Fang, Tony Jun Huang   +5 more
openaire   +3 more sources

Recent Advances in Dielectrophoretic Manipulation and Separation of Microparticles and Biological Cells

Biosensors
Dielectrophoresis (DEP) is an advanced microfluidic manipulation technique that is based on the interaction of polarized particles with the spatial gradient of a non-uniform electric field to achieve non-contact and highly selective manipulation of ...
Junzhu Yao, Kai Zhao, Jia Lou, Kaihuan Zhang   +3 more
doaj   +1 more source

Sheathless Dielectrophoresis-Based Microfluidic Chip for Label-Free Bio-Particle Focusing and Separation

Engineering Proceedings, 2023
This paper presents a novel microfluidic dielectrophoresis (DEP) system to focus and separate cells of similar size based on their structural differences, which is more challenging than separation by size.
Reza Vamegh, Zeynab Alipour, Mehdi Fardmanesh   +2 more
doaj   +1 more source

Structural and mechanistic basis for the regulation of the chloroplast signal recognition particle by (p)ppGpp

FEBS Letters, EarlyView.
LHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Victor Zegarra, Paul Weiland, Pauline Anka Plitzko, Julia Thiery, Laura Czech, Felix Willmund, Patricia Bedrunka, Gert Bange   +7 more
wiley   +1 more source