Results 41 to 50 of about 216,120 (296)

Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays [PDF]

, 2018
This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture ...
Daza Navarro, María Paula, Huertas Sánchez, Gloria, Maldonado-Jacobi, Andrés, Martín Rubio, María Esther, Olmo Fernández, Alberto, Pérez García, Pablo, Serrano, Juan A., Yúfera García, Alberto   +7 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Conserved structural motifs in PAS, LOV, and CRY proteins regulate circadian rhythms and are therapeutic targets

FEBS Letters, EarlyView.
Cryptochrome and PAS/LOV proteins play intricate roles in circadian clocks where they act as both sensors and mediators of protein–protein interactions. Their ubiquitous presence in signaling networks has positioned them as targets for small‐molecule therapeutics. This review provides a structural introduction to these protein families.
Eric D. Brinckman, Anna E. Lester, Brian D. Zoltowski   +2 more
wiley   +1 more source

The speed of FtsZ treadmilling is tightly regulated by membrane binding

Scientific Reports, 2020
As one of the key elements in bacterial cell division, the cytoskeletal protein FtsZ appears to be highly involved in circumferential treadmilling along the inner membrane, yielding circular vortices when transferred to flat membranes.
Daniela A. García-Soriano, Tamara Heermann, Ana Raso, Germán Rivas, Petra Schwille   +4 more
doaj   +1 more source

Elucidation of molecular kinetic schemes from macroscopic traces using system identification [PDF]

, 2017
Overall cellular responses to biologically-relevant stimuli are mediated by networks of simpler lower-level processes. Although information about some of these processes can now be obtained by visualizing and recording events at the molecular level, this
Brezina, Vladimir, Fribourg, Miguel, Galocha-Iragüen, Belén, González-Maeso, Javier, Las-Heras Andrés, Fernando, Logothetis, Diomedes E., Sealfon, Stuart C.   +6 more
core   +6 more sources

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Engineered depalmitoylases enable selective manipulation of protein localization and function

Nature Communications
S-Palmitoylation is a reversible post-translational modification that tunes the localization, stability, and function of an impressive array of proteins including ion channels, G-proteins, and synaptic proteins.
Srinidhi Jayaraman, Audrey Kochiss, Thy-Lan Alcalay, Pedro J. del Rivero Morfin, Manu Ben-Johny   +4 more
doaj   +1 more source

On the phase space structure of IP3 induced Ca2+ signalling and concepts for predictive modeling [PDF]

, 2018
The correspondence between mathematical structures and experimental systems is the basis of the generalizability of results found with specific systems, and is the basis of the predictive power of theoretical physics.
Agne Tilūnaitė, Alexander Skupin, Berridge M., Bootman M., Dupont G., Dupont G., Goldbeter A., Keener J., Mahsa Moein, Martin Falcke, Murray J., Rooney T., Rüdiger Thul, Skupin A., Skupin A.   +14 more
core   +4 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Reconstitution of contractile actomyosin rings in vesicles

Nature Communications, 2021
Cytoskeletal networks support and direct cell shape and guide intercellular transport, but relatively little is understood about the self-organization of cytoskeletal components on the scale of an entire cell.
Thomas Litschel, Charlotte F. Kelley, Danielle Holz, Maral Adeli Koudehi, Sven K. Vogel, Laura Burbaum, Naoko Mizuno, Dimitrios Vavylonis, Petra Schwille   +8 more
doaj   +1 more source