Reporter Group-Labeled Synthetic Cellulose: Structural Characterization and Utilization in Mapping the Cellulose Chain-Cleavage Modes of Cellulases. [PDF]
Kaira GS +3 more
europepmc +1 more source
鑑於石油枯竭的可能性,尋找可再生的替代能源市一項非常重要的課題。生質酒精(bioethanol)市其中一個非常具有開發價值的生質能源。其主要方法是將纖維素(cellulose)醣化後,再經由發酵生成酒精。纖維素是自然界中分佈最廣泛,含量最龐大的一種多醣,也是植物細胞壁的主要成份。木質纖維素(lignocellulose)之組成主要是纖維素,半纖維素(hemicellulose)和木質素(lignin)。由於纖維素被層層包覆在木質纖維素的複雜結構中,導致其無法被纖維素酵素(cellulase)水解。厭氧菌
Cally Yap Joe San, 葉佐珊
core
Studies of anaerobic fungus Neocallimastix patriciarum J11 cellulosome system
本研究自以結晶性纖維Avicel為碳源的厭氣性真菌Neocallimastix patriciarum J11培養上清液中純化分子量大於669 kDa且具纖維素分解酶活性之大分子蛋白聚合物,經變性聚丙烯醯胺膠體電泳及酵素活性染色分析,結果呈現結果呈現12條蛋白質,其中8條具纖維素分解酶活性、4條具木聚醣分解酶活性。由此結果顯示,N.
Wang, Hui-Chang, 王惠昌
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Deconstruction by <i>C. thermocellum</i>-from microbe mediated to dynamic redistribution of cellulosomes. [PDF]
Yarbrough JM +9 more
europepmc +1 more source
Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability
Background: The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes.
Moraïs, S. +9 more
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Characterization of a Double Dockerin from the Cellulosome of the Anaerobic Fungus Piromyces equi
The assembly into supramolecular complexes of proteins having complementary activities is central to cellular function. One such complex of considerable biological and industrial significance is the plant cell wall-degrading apparatus of anaerobic ...
Gilbert HJ +5 more
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Advancing cellulose degradation through synthetic biology: engineered pathways and microbial systems for sustainable biomass conversion. [PDF]
Liu X, Quan J, Li Y, Wang X, Zhao J.
europepmc +1 more source
Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum.
International audienceThe cellulolytic complex was isolated from Clostridium cellulolyticum grown on cellulose. Upon gel filtration, the complex was found to consist mainly of 600-kDa units, along with a 16-MDa aggregate.
Belaich, Jean-Pierre +6 more
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Proteomic Characterization of the <i>Clostridium cellulovorans</i> Cellulosome and Noncellulosomal Enzymes with Sorghum Bagasse. [PDF]
Eljonid MY +5 more
europepmc +1 more source
Fungal-bacterial interaction networks in lignocellulose degradation: an integrated ecological and mechanistic perspective. [PDF]
Lee YY, Yun J.
europepmc +1 more source

