Results 91 to 100 of about 3,121 (198)

Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

open access: yesBiotechnology for Biofuels, 2018
Background Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called ‘cellulosome’.
Jui-Jen Chang   +9 more
doaj   +1 more source

Recombinant Cellulase and Cellulosome Systems [PDF]

open access: yes, 2013
The non-renewable fossil resources currently exploited by the oil and gas industries are the objects of growing concern owing to their finite supply and contribution to global warming. Lignocellulosic biomass is a sustainable alternative to fossil resources, and has the added advantage of not competing with human and animal nutrition.
Andrew S.   +2 more
openaire   +1 more source

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

open access: yes, 2017
\ua9 2017 The Author(s). Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Carvalho AL   +9 more
core  

Glycosylation of hyperthermostable designer cellulosome components yields enhanced stability and cellulose hydrolysis [PDF]

open access: yes, 2020
Biomass deconstruction remains integral for enabling second‐generation biofuel production at scale. However, several steps necessary to achieve significant solubilization of biomass, notably harsh pretreatment conditions, impose economic barriers to ...
Moraïs, Sarah   +8 more
core  

Potential of Selected Rumen Bacteria for Cellulose and Hemicellulose Degradation

open access: yesFood Technology and Biotechnology, 2014
Herbivorous animals harbour potent cellulolytic and hemicellulolytic microorganisms that supply the host with nutrients acquired from degradation of ingested plant material. In addition to protozoa and fungi, rumen bacteria contribute a considerable part
Maša Zorec   +2 more
doaj  

Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

open access: yesMicrobial Cell Factories, 2010
Background The assembly and spatial organization of enzymes in naturally occurring multi-protein complexes is of paramount importance for the efficient degradation of complex polymers and biosynthesis of valuable products.
Wieczorek Andrew S, Martin Vincent JJ
doaj   +1 more source

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis.

open access: yesPLoS ONE, 2015
Degradation of cellulose is of major interest in the quest for alternative sources of renewable energy, for its positive effects on environment and ecology, and for use in advanced biotechnological applications.
Johanna Stern   +6 more
doaj   +1 more source

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

open access: yesmBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern   +3 more
doaj   +1 more source

Molecular Architecture and Structural Transitions of a Clostridium thermocellum Mini-Cellulosome

open access: yes, 2011
10 páginas, 4 figuras, 3 suplementos figuras -- PAGS nros. 571-580The cellulosome is a highly elaborate cell-bound multienzyme complex that efficiently orchestrates the deconstruction of cellulose and hemicellulose, two of the nature's most abundant ...
Llorca, Óscar   +17 more
core   +1 more source

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

open access: yesPLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman   +7 more
doaj   +1 more source

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