Results 71 to 80 of about 2,416 (170)

Enhancement of Consolidated Bioprocessing for Bioethanol Conversion From Water Hyacinth Using the Wild and Mutant Bacterial Strains

International Journal of Chemical Engineering, Volume 2025, Issue 1, 2025.
Bacterial strains Hangateiclostridium thermocellum KSMK1203 and Lachnoclostridium phytofermentans KSM 1203 were subjected to physiochemical mutagenesis to improve their capacity for cellulose degradation, boost ethanol production, and enhance their resilience against various substrates and byproducts.
Kavitha S., Selvakumar Periyasamy, Gajendran T., Yuvaraj S., Arnab Biswas   +4 more
wiley   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source

GASdb: a large-scale and comparative exploration database of glycosyl hydrolysis systems

BMC Microbiology, 2010
Background The genomes of numerous cellulolytic organisms have been recently sequenced or in the pipeline of being sequenced. Analyses of these genomes as well as the recently sequenced metagenomes in a systematic manner could possibly lead to ...
Xu Ying, Chen Huiling, Zhou Fengfeng
doaj   +1 more source

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Scientific Reports, 2018
Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes   +11 more
doaj   +1 more source

Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

Biotechnology for Biofuels, 2018
Background Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called ‘cellulosome’.
Jui-Jen Chang, Marimuthu Anandharaj, Cheng-Yu Ho, Kenji Tsuge, Tsung-Yu Tsai, Huei-Mien Ke, Yu-Ju Lin, Minh Dung Ha Tran, Wen-Hsiung Li, Chieh-Chen Huang   +9 more
doaj   +1 more source

Potential of Selected Rumen Bacteria for Cellulose and Hemicellulose Degradation

Food Technology and Biotechnology, 2014
Herbivorous animals harbour potent cellulolytic and hemicellulolytic microorganisms that supply the host with nutrients acquired from degradation of ingested plant material. In addition to protozoa and fungi, rumen bacteria contribute a considerable part
Maša Zorec, Maša Vodovnik, Romana Marinšek-Logar   +2 more
doaj  

Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

Microbial Cell Factories, 2010
Background The assembly and spatial organization of enzymes in naturally occurring multi-protein complexes is of paramount importance for the efficient degradation of complex polymers and biosynthesis of valuable products.
Wieczorek Andrew S, Martin Vincent JJ
doaj   +1 more source

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis.

PLoS ONE, 2015
Degradation of cellulose is of major interest in the quest for alternative sources of renewable energy, for its positive effects on environment and ecology, and for use in advanced biotechnological applications.
Johanna Stern, Amaranta Kahn, Yael Vazana, Melina Shamshoum, Sarah Moraïs, Raphael Lamed, Edward A Bayer   +6 more
doaj   +1 more source

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

mBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern, Sarah Moraïs, Raphael Lamed, Edward A. Bayer   +3 more
doaj   +1 more source

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

PLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman, Chongle Pan, Gregory B Hurst, Miguel Rodriguez, Catherine K McKeown, Patricia K Lankford, Nagiza F Samatova, Jonathan R Mielenz   +7 more
doaj   +1 more source