Results 51 to 60 of about 3,121 (198)
Enhanced Microbial Utilization of Recalcitrant Cellulose by an Ex Vivo Cellulosome-Microbe Complex
, 2011 A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the ...
Lynd, Lee R +3 more
core +1 more source
International Journal of Biological MacromoleculesThe development of cellulases cocktails to efficiently solubilize cellulose for biotechnological applications is a constant demand. Cellulases exhibit diverse mode of action, operating in the free state or in multi-enzyme complexes (cellulosomes), and potentially acting synergistically for a higher efficiency.
David, Hélène +6 more
openaire +4 more sources
Cellulosomics of the cellulolytic thermophile Clostridium clariflavum [PDF]
Biotechnology for Biofuels, 2014 Clostridium clariflavum is an anaerobic, thermophilic, Gram-positive bacterium, capable of growth on crystalline cellulose as a single carbon source. The genome of C. clariflavum has been sequenced to completion, and numerous cellulosomal genes were identified, including putative scaffoldin and enzyme subunits.Bioinformatic analysis of the C ...
Artzi, Lior +5 more
openaire +2 more sources
, 2018 Recombinant Bacillus subtilis WB800N harboring pGETS 118 designer cellulosome genes was applied to the artificial cellulosome production. To investigate the characteristics of cellulosome assembly in the supernatant, three free enzymes clones, i.e ...
Liang-Yu Yang +8 more
core +1 more source
Green CarbonThe bioconversion of lignocellulose has attracted global attention, due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon.
Min Xiao +5 more
doaj +1 more source
Impact of scaffoldin mechanostability on cellulosomal activity
Biomaterials Science, 2020 A low mechanostability cohesin between the two cellulosomal anchoring points lowers saccharification activity (top), in contrast to alternative configurations (bottom).
Albert Galera-Prat +5 more
openaire +4 more sources
Protein Science, Volume 35, Issue 7, July 2026.Abstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Iker Pardo Larrabeiti +11 more
wiley +1 more source
The Cellulosome Paradigm in An Extreme Alkaline Environment
Microorganisms, 2019 Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates cellulolytic activity.
Paripok Phitsuwan +4 more
openaire +6 more sources
Organization and distribution of the cellulosome in Clostridium thermocellum [PDF]
Journal of Bacteriology, 1985 The properties of the cellulosome (the cellulose-binding, multicellulase-containing protein complex) in Clostridium thermocellum were examined by comparing the cellulase systems derived from the wild type and an adherence-defective mutant. The growth conditions--specifically, growth either on cellulose (Avicel) or on cellobiose as insoluble or soluble ...
E A, Bayer, E, Setter, R, Lamed
openaire +2 more sources
Toward Efficient Cellulosic Biomass Breakdown: Enzymatic Innovation and Kinetic Insights
Macromolecular Materials and Engineering, Volume 311, Issue 6, June 2026.By combining a processive GH7, CBM2a, and GH3 into single enzyme, the GCG fusion protein effectively breaks down crystalline cellulose. This tri‐domain architecture reduces cellobiose inhibition at the cellulose surface allowing for the simultaneous targeting of the substrate, depolymerization, and removal of products.
Anoth Maharjan +4 more
wiley +1 more source