Results 31 to 40 of about 2,416 (170)
Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase [PDF]
FEBS Letters, 1998 Activity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it.
Ciruela, Antonio +3 more
openaire +2 more sources
Current challenges in designer cellulosome engineering
Applied Microbiology and Biotechnology, 2023 Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic
Babette Lamote +5 more
openaire +3 more sources
Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation
Biotechnology for Biofuels, 2012 Background Microorganisms employ a multiplicity of enzymes to efficiently degrade the composite structure of plant cell wall cellulosic polysaccharides.
Moraïs Sarah +6 more
doaj +1 more source
Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold. [PDF]
Microb BiotechnolThe first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Elias M +4 more
europepmc +2 more sources
Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum [PDF]
Journal of Bacteriology, 2004 ABSTRACT The man5K gene of Clostridium cellulolyticum was cloned and overexpressed in Escherichia coli . This gene encodes a 424-amino-acid preprotein composed of an N-terminal leader peptide, followed by a dockerin module and a C-terminal catalytic module ...
Stéphanie, Perret +4 more
openaire +2 more sources
Cellulosomal expansin: functionality and incorporation into the complex [PDF]
Biotechnology for Biofuels, 2016 Expansins are relatively small proteins that lack enzymatic activity and are found in plants and microorganisms. The function of these proteins is to disrupt the plant cell walls by interfering with the non-covalent interchain bonding of the polysaccharides.
Lior Artzi +3 more
openaire +2 more sources
Computational and Structural Biotechnology Journal, 2012 Cellulose waste biomass is the most abundant and attractive substrate for “biorefinery strategies” that are aimed to produce high-value products (e.g.
Roberto Mazzoli
doaj +3 more sources
Predicting Selective RNA Processing and Stabilization Operons in Clostridium spp.
Frontiers in Microbiology, 2021 In selective RNA processing and stabilization (SRPS) operons, stem–loops (SLs) located at the 3′-UTR region of selected genes can control the stability of the corresponding transcripts and determine the stoichiometry of the operon. Here, for such operons,
Yogendra Bhaskar +5 more
doaj +1 more source
Frontiers in Microbiology, 2022 As part of free cellulases or scaffolding proteins in cellulosomes, the hydrophilic non-catalytic X2 module is widely distributed in cellulolytic Clostridia or other Firmicutes bacteria. Previous biochemical studies suggest that X2 modules might increase
Xuanyu Tao +8 more
doaj +1 more source
Nanoscale resolution of microbial fiber degradation in action
eLife, 2022 The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology.
Meltem Tatli +6 more
doaj +1 more source