Results 31 to 40 of about 3,121 (198)

A dual cohesin-dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome [PDF]

, 2021
The Cellulosome is an intricate macromolecular protein complex that centralizes the cellulolytic efforts of many anaerobic microorganisms through the promotion of enzyme synergy and protein stability.
Bule, Pedro, Cabrita, Eurico J., Carvalho, Ana Luísa, Ferreira, Luís M. A., Duarte, Marlene, Prates, José A. M., Fontes, Carlos M. G. A., Alves, Victor D., Viegas, Aldino, Najmudin, Shabir   +9 more
core   +1 more source

Efficient yeast surface-display of novel complex synthetic cellulosomes

Microbial Cell Factories, 2018
Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang, Jiajing Wang, Shenghuan Wang, Yu Shen, Dina Petranovic, Jin Hou, Xiaoming Bao   +6 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +1 more source

Colocalization and Disposition of Cellulosomes in Clostridium clariflavum as Revealed by Correlative Superresolution Imaging

mBio, 2018
Cellulosomes are multienzyme complexes produced by anaerobic, cellulolytic bacteria for highly efficient breakdown of plant cell wall polysaccharides.
Lior Artzi, Tali Dadosh, Elad Milrot, Sarah Moraïs, Smadar Levin-Zaidman, Ely Morag, Edward A. Bayer   +6 more
doaj   +1 more source

Cellulase-Xylanase Synergy in Designer Cellulosomes for Enhanced Degradation of a Complex Cellulosic Substrate

mBio, 2010
Designer cellulosomes are precision-engineered multienzyme complexes in which the molecular architecture and enzyme content are exquisitely controlled.
Sarah Moraïs, Yoav Barak, Jonathan Caspi, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +7 more
doaj   +1 more source

Nanoscale Engineering of Designer Cellulosomes

Advanced Materials, 2016
Biocatalysts showcase the upper limit obtainable for high‐speed molecular processing and transformation. Efforts to engineer functionality in synthetic nanostructured materials are guided by the increasing knowledge of evolving architectures, which enable controlled molecular motion and precise molecular recognition.
Gunnoo M., Cazade P.-A., Galera-Prat A., Nash M.A., Czjzek M., Cieplak M., Alvarez B., Aguilar M., Karpol A., Gaub H., Carrión-Vázquez M., Bayer E.A., Thompson D.   +12 more
openaire   +4 more sources

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome-Based Synthetic Protein Scaffold. [PDF]

Microb Biotechnol
The first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Elias M, Meert K, Vanderstraeten J, Lamote B, Briers Y.   +4 more
europepmc   +2 more sources

Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

Microorganisms, 2017
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs   +6 more
doaj   +1 more source

Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum [PDF]

Journal of Bacteriology, 2004
ABSTRACT The man5K gene of Clostridium cellulolyticum was cloned and overexpressed in Escherichia coli . This gene encodes a 424-amino-acid preprotein composed of an N-terminal leader peptide, followed by a dockerin module and a C-terminal catalytic module ...
Stéphanie, Perret, Anne, Bélaich, Henri-Pierre, Fierobe, Jean-Pierre, Bélaich, Chantal, Tardif   +4 more
openaire   +2 more sources

DEVELOPMENT OF MICROORGANISMS FOR CELLULOSE-BIOFUEL CONSOLIDATED BIOPROCESSINGS: METABOLIC ENGINEERS' TRICKS

Computational and Structural Biotechnology Journal, 2012
Cellulose waste biomass is the most abundant and attractive substrate for “biorefinery strategies” that are aimed to produce high-value products (e.g.
Roberto Mazzoli
doaj   +3 more sources