Results 11 to 20 of about 3,492 (148)

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Biotechnology for Biofuels, 2017
Background (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B.
Olga Zhivin, Bareket Dassa, Sarah Moraïs, Sagar M. Utturkar, Steven D. Brown, Bernard Henrissat, Raphael Lamed, Edward A. Bayer   +7 more
doaj   +2 more sources

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Scientific Reports, 2017
Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule, Victor D. Alves, Vered Israeli-Ruimy, Ana L. Carvalho, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M. G. A. Fontes   +9 more
doaj   +2 more sources

Creation of a functional hyperthermostable designer cellulosome [PDF]

Biotechnology for Biofuels, 2019
Background Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy.
Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer   +9 more
doaj   +4 more sources

Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

BMC Genomics, 2012
Background Microbial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate ...
Dassa Bareket, Borovok Ilya, Lamed Raphael, Henrissat Bernard, Coutinho Pedro, Hemme Christopher L, Huang Yue, Zhou Jizhong, Bayer Edward A   +8 more
doaj   +2 more sources

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

mBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni, Sarah Moraïs, Yonit Ben-David, Ely Morag, Edward A. Bayer   +4 more
doaj   +2 more sources

How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

Biotechnology for Biofuels, 2017
Background Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic ...
Shahar Yoav, Yoav Barak, Melina Shamshoum, Ilya Borovok, Raphael Lamed, Bareket Dassa, Yitzhak Hadar, Ely Morag, Edward A. Bayer   +8 more
doaj   +2 more sources

Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization. [PDF]

Protein Sci
Abstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Pardo Larrabeiti I, Eibinger M, Esque J, Pradeau S, Fort S, Moraïs S, Mizrahi I, Bayer EA, Nidetzky B, Montanier CY, Roblin P, Dumon C.   +11 more
europepmc   +2 more sources

Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study

Biotechnology for Biofuels and Bioproducts, 2022
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten, Maria João Maurício da Fonseca, Philippe De Groote, Dennis Grimon, Hans Gerstmans, Amaranta Kahn, Sarah Moraïs, Edward A. Bayer, Yves Briers   +8 more
doaj   +1 more source

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria. [PDF]

Angew Chem Int Ed Engl
The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Ishida K, Krabbe J, Meisinger PR, Shabuer G, Schieferdecker S, Cyrulies M, Tank C, Barnes E, Paetz C, Hertweck C.   +9 more
europepmc   +3 more sources

Enzymatic Characterization of Unused Biomass Degradation Using the Clostridium cellulovorans Cellulosome

Microorganisms, 2022
The cellulolytic system of Clostridium cellulovorans mainly consisting of a cellulosome that synergistically collaborates with non-complexed enzymes was investigated using cellulosic biomass.
Mohamed Yahia Eljonaid, Hisao Tomita, Fumiyoshi Okazaki, Yutaka Tamaru   +3 more
doaj   +1 more source