Results 11 to 20 of about 3,121 (198)

Creation of a functional hyperthermostable designer cellulosome [PDF]

Biotechnology for Biofuels, 2019
Background Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy.
Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer   +9 more
doaj   +8 more sources

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Biotechnology for Biofuels, 2017
Background (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B.
Olga Zhivin, Bareket Dassa, Sarah Moraïs, Sagar M. Utturkar, Steven D. Brown, Bernard Henrissat, Raphael Lamed, Edward A. Bayer   +7 more
doaj   +2 more sources

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Scientific Reports, 2017
Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule, Victor D. Alves, Vered Israeli-Ruimy, Ana L. Carvalho, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M. G. A. Fontes   +9 more
doaj   +2 more sources

Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

BMC Genomics, 2012
Background Microbial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate ...
Dassa Bareket, Borovok Ilya, Lamed Raphael, Henrissat Bernard, Coutinho Pedro, Hemme Christopher L, Huang Yue, Zhou Jizhong, Bayer Edward A   +8 more
doaj   +2 more sources

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

mBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni, Sarah Moraïs, Yonit Ben-David, Ely Morag, Edward A. Bayer   +4 more
doaj   +2 more sources

How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

Biotechnology for Biofuels, 2017
Background Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic ...
Shahar Yoav, Yoav Barak, Melina Shamshoum, Ilya Borovok, Raphael Lamed, Bareket Dassa, Yitzhak Hadar, Ely Morag, Edward A. Bayer   +8 more
doaj   +2 more sources

Comparison of the mesophilic cellulosome‐producing Clostridium cellulovorans genome with other cellulosome‐related clostridial genomes [PDF]

Microbial Biotechnology, 2010
Summary Clostridium cellulovorans , an anaerobic and mesophilic bacterium, degrades native substrates in soft biomass such as corn fibre and rice straw efficiently by producing an extracellular enzyme complex called the cellulosome. Recently, we have reported the whole‐genome sequence of
Tamaru, Yutaka, Miyake, Hideo, Kuroda, Kouichi, Nakanishi, Akihito, Matsushima, Chiyuki, Doi, Roy H, Ueda, Mitsuyoshi   +6 more
openaire   +6 more sources

Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase [PDF]

FEBS Letters, 1998
Activity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it.
Ciruela, Antonio, Gilbert, Harry J, Ali, Bassam R.S, Hazlewood, Geoffrey P   +3 more
openaire   +3 more sources

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome [PDF]

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +2 more sources

Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization

Biotechnology for Biofuels, 2018
Background The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplished with fungal cellulases, whereas
Benedikt Leis, Claudia Held, Björn Andreeßen, Wolfgang Liebl, Sigrid Graubner, Louis-Philipp Schulte, Wolfgang H. Schwarz, Vladimir V. Zverlov   +7 more
doaj   +2 more sources