Results 21 to 30 of about 3,492 (148)

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Diverse specificity of cellulosome attachment to the bacterial cell surface [PDF]

During the course of evolution, the cellulosome, one of Nature\u27s most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently deconstruct recalcitrant carbohydrates.
Pires VMR, Romao MJ, Fontes CMGA, Spencer HL, Viegas A, Cuskin F, Cameron K, Carvalho AL, Smith S, Gilbert HJ, Najmudin S, Alves VD, Bayer EA, Bras JLA, Pinheiro BA, Bule P   +15 more
core   +4 more sources

Enhanced Microbial Utilization of Recalcitrant Cellulose by an Ex Vivo Cellulosome-Microbe Complex [PDF]

, 2011
A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the ...
Lynd, Lee R, Zhang, Xiao-Zhou, Sathitsuksanoh, Noppadon, You, Chun   +3 more
core   +1 more source

Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake

Frontiers in Microbiology, 2021
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential.
Timothy J. Williams, Michelle A. Allen, Jonathan F. Berengut, Ricardo Cavicchioli   +3 more
doaj   +1 more source

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu, Jie Lu, Guodong Wang, Guodong Wang, Cuiping Yang, Cuiping Yang, Zehao Peng, Zehao Peng, Lu Yang, Lu Yang, Bowen Du, Bowen Du, Chuanzhuang Guo, Songsen Sui, Jianbin Wang, Junlin Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang   +19 more
doaj   +1 more source

Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries

Fermentation, 2023
Clostridium cellulolyticum H10 (ATCC 35319) has the ability to ferment cellulosic substrates into ethanol and weak acids. The growth and alcohol production rates of the wild-type organism are low and, therefore, targets of metabolic engineering.
Benjamin G. Freedman, Parker W. Lee, Ryan S. Senger   +2 more
doaj   +1 more source

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

, 2017
\ua9 2017 The Author(s). Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Carvalho AL, Fontes CMGA, Smith SP, Gilbert HJ, Najmudin S, Alves VD, Israeli-Ruimy V, Ferreira LMA, Bayer EA, Bule P   +9 more
core   +4 more sources

Efficient yeast surface-display of novel complex synthetic cellulosomes

Microbial Cell Factories, 2018
Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang, Jiajing Wang, Shenghuan Wang, Yu Shen, Dina Petranovic, Jin Hou, Xiaoming Bao   +6 more
doaj   +1 more source

Colocalization and Disposition of Cellulosomes in Clostridium clariflavum as Revealed by Correlative Superresolution Imaging

mBio, 2018
Cellulosomes are multienzyme complexes produced by anaerobic, cellulolytic bacteria for highly efficient breakdown of plant cell wall polysaccharides.
Lior Artzi, Tali Dadosh, Elad Milrot, Sarah Moraïs, Smadar Levin-Zaidman, Ely Morag, Edward A. Bayer   +6 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +1 more source