Results 21 to 30 of about 2,416 (170)

Genome-wide analysis of acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system

BMC Genomics, 2012
Background Microbial degradation of plant cell walls and its conversion to sugars and other byproducts is a key step in the carbon cycle on Earth. In order to process heterogeneous plant-derived biomass, specialized anaerobic bacteria use an elaborate ...
Dassa Bareket, Borovok Ilya, Lamed Raphael, Henrissat Bernard, Coutinho Pedro, Hemme Christopher L, Huang Yue, Zhou Jizhong, Bayer Edward A   +8 more
doaj   +1 more source

Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries

Fermentation, 2023
Clostridium cellulolyticum H10 (ATCC 35319) has the ability to ferment cellulosic substrates into ethanol and weak acids. The growth and alcohol production rates of the wild-type organism are low and, therefore, targets of metabolic engineering.
Benjamin G. Freedman, Parker W. Lee, Ryan S. Senger   +2 more
doaj   +1 more source

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Scientific Reports, 2017
Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule, Victor D. Alves, Vered Israeli-Ruimy, Ana L. Carvalho, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M. G. A. Fontes   +9 more
doaj   +1 more source

Efficient yeast surface-display of novel complex synthetic cellulosomes

Microbial Cell Factories, 2018
Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang, Jiajing Wang, Shenghuan Wang, Yu Shen, Dina Petranovic, Jin Hou, Xiaoming Bao   +6 more
doaj   +1 more source

Colocalization and Disposition of Cellulosomes in Clostridium clariflavum as Revealed by Correlative Superresolution Imaging

mBio, 2018
Cellulosomes are multienzyme complexes produced by anaerobic, cellulolytic bacteria for highly efficient breakdown of plant cell wall polysaccharides.
Lior Artzi, Tali Dadosh, Elad Milrot, Sarah Moraïs, Smadar Levin-Zaidman, Ely Morag, Edward A. Bayer   +6 more
doaj   +1 more source

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +1 more source

Cellulase-Xylanase Synergy in Designer Cellulosomes for Enhanced Degradation of a Complex Cellulosic Substrate

mBio, 2010
Designer cellulosomes are precision-engineered multienzyme complexes in which the molecular architecture and enzyme content are exquisitely controlled.
Sarah Moraïs, Yoav Barak, Jonathan Caspi, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +7 more
doaj   +1 more source

Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

Microorganisms, 2017
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs   +6 more
doaj   +1 more source

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

mBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni, Sarah Moraïs, Yonit Ben-David, Ely Morag, Edward A. Bayer   +4 more
doaj   +1 more source

Assembly of Xylanases into Designer Cellulosomes Promotes Efficient Hydrolysis of the Xylan Component of a Natural Recalcitrant Cellulosic Substrate

mBio, 2011
In nature, the complex composition and structure of the plant cell wall pose a barrier to enzymatic degradation. Nevertheless, some anaerobic bacteria have evolved for this purpose an intriguing, highly efficient multienzyme complex, the cellulosome ...
Sarah Moraïs, Yoav Barak, Yitzhak Hadar, David B. Wilson, Yuval Shoham, Raphael Lamed, Edward A. Bayer   +6 more
doaj   +1 more source