Results 21 to 30 of about 3,121 (198)

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]

PeerJ, 2014
Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg, Vered Ruimy-Israeli, Bareket Dassa, Yoav Barak, Raphael Lamed, Kate Cameron, Carlos M.G.A. Fontes, Edward A. Bayer, Daniel B. Fried   +8 more
doaj   +3 more sources

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria. [PDF]

Angew Chem Int Ed Engl
The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Ishida K, Krabbe J, Meisinger PR, Shabuer G, Schieferdecker S, Cyrulies M, Tank C, Barnes E, Paetz C, Hertweck C.   +9 more
europepmc   +3 more sources

Creating a large designer cellulosome in yeast to boost ethanol production. [PDF]

Proc Natl Acad Sci U S A
Cellulosic biomass represents a promising feedstock for biofuel and biochemical production. However, its recalcitrant structure strongly hinders enzymatic degradation. Cellulosomes are large multienzyme complexes, highly efficient at degrading cellulose.
Khatoon Z, Anandharaj M, Chen TH, Wu CC, Cheng JF, Hsu TL, Lin HC, Chang JJ, Li WH.   +8 more
europepmc   +2 more sources

Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study

Biotechnology for Biofuels and Bioproducts, 2022
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different
Julie Vanderstraeten, Maria João Maurício da Fonseca, Philippe De Groote, Dennis Grimon, Hans Gerstmans, Amaranta Kahn, Sarah Moraïs, Edward A. Bayer, Yves Briers   +8 more
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake

Frontiers in Microbiology, 2021
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential.
Timothy J. Williams, Michelle A. Allen, Jonathan F. Berengut, Ricardo Cavicchioli   +3 more
doaj   +1 more source

Extracytoplasmic polysaccharides control cellulosomal and non-cellulosomal systems in Herbivorax saccincola A7. [PDF]

Appl Microbiol Biotechnol
Herbivorax saccincola A7 is an anaerobic alkali-thermophilic lignocellulolytic bacterium that possesses a cellulosome and high xylan degradation ability. To understand the expression profile of extracellular enzymes by carbon sources, quantitative real-time PCR was performed on all cellulosomal and non-cellulosomal enzyme genes of H.
Baramee S, Thianheng P, Uke A, Cheawchanlertfa P, Tachaapaikoon C, Waeonukul R, Pason P, Ratanakhanokchai K, Liu YJ, Kosugi A.   +9 more
europepmc   +3 more sources

Cellulosomes from Mesophilic Bacteria [PDF]

Journal of Bacteriology, 2003
Plant cell wall-degrading enzymes have become increasingly important, since the development of efficient biomass degradation methods and the conversion of sugars to valuable products such as butanol and amino acids and utilizable forms of energy such as ethanol and methane could lead to less dependence on imported petroleum as a fuel and chemical ...
Roy H, Doi, Akihiko, Kosugi, Koichiro, Murashima, Yutaka, Tamaru, Sung Ok, Han   +4 more
openaire   +2 more sources

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu, Jie Lu, Guodong Wang, Guodong Wang, Cuiping Yang, Cuiping Yang, Zehao Peng, Zehao Peng, Lu Yang, Lu Yang, Bowen Du, Bowen Du, Chuanzhuang Guo, Songsen Sui, Jianbin Wang, Junlin Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang   +19 more
doaj   +1 more source

Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries

Fermentation, 2023
Clostridium cellulolyticum H10 (ATCC 35319) has the ability to ferment cellulosic substrates into ethanol and weak acids. The growth and alcohol production rates of the wild-type organism are low and, therefore, targets of metabolic engineering.
Benjamin G. Freedman, Parker W. Lee, Ryan S. Senger   +2 more
doaj   +1 more source