Results 61 to 70 of about 3,492 (148)

Spatio‐temporal characteristics of the gastrointestinal resistome in a cow‐to‐calf model and its environmental dissemination in a dairy production system

iMeta, Volume 4, Issue 4, August 2025.
This study revealed spatio‐temporal characteristics of gastrointestinal resistome in a cow‐to‐calf model and the pattern of their spread to the environment and offspring in the dairy production system. The gastrointestinal tract of dairy cows is a natural reservoir of resistomes and distinguished by gut sites and regions.
Shuai Liu, Yimin Zhuang, Tianyu Chen, Duo Gao, Jianxin Xiao, Jinfeng Wang, Jinghui Li, Xinjie Zhao, Rong Peng, Wenli Guo, Jialin Wei, Mo Sha, Jingjun Wang, Jiaying Ma, Mei Ma, Mengmeng Li, Wei Wang, Ya‐Jing Wang, Shengli Li, Zhijun Cao   +19 more
wiley   +1 more source

Grape Stalks Valorization towards Circular Economy: A Cascade Biorefinery Strategy

ChemSusChem, Volume 18, Issue 10, May 19, 2025.
A cascade protocol for grape stalks valorization has been proposed, characterized by a multidisciplinary strategy using unconventional techniques and subcritical water as a green solvent, where physical/chemical treatments synergistically interact with biological treatments to obtain added value products from all the biomass components.
Carlotta Valle, Giorgio Grillo, Emanuela Calcio Gaudino, Paola Ponsetto, Roberto Mazzoli, Giulia Bonavita, Pietro Vitale, Enrica Pessione, Emilia Garcia‐Moruno, Antonella Costantini, Giancarlo Cravotto, Silvia Tabasso   +11 more
wiley   +1 more source

Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold

Microbial Biotechnology, Volume 18, Issue 4, April 2025.
The first three Escherichia coli glycolytic enzymes were converted to the docking enzyme mode and subsequently scaffolded. Increasing copies of the rate‐limiting enzyme boosted the overall performance. At lower enzyme densities, the proximity effects provided by scaffold‐colocalisation result in a higher product yield compared to free docking enzymes ...
Marte Elias, Kenan Meert, Julie Vanderstraeten, Babette Lamote, Yves Briers   +4 more
wiley   +1 more source

Designing chimeric enzymes inspired by fungal cellulosomes

Synthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore, Stephen P. Lillington, Charles H. Haitjema, Randall de Groot, Michelle A. O'Malley   +4 more
doaj   +1 more source

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization

Engineering in Life Sciences, Volume 25, Issue 3, March 2025.
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauss   +2 more
wiley   +1 more source

Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation

, 2016
Cellulosomes are multi-enzyme complexes assembled by cellulases and hemicellulases through dockerin-cohesin interactions, which are the most efficient system for the degradation of lignocellulosic resources in nature.
Song, Xiangfei, Cui, Qiu, Chen, Chao, Liu, Ya-Jun, Cui, Zhenling, Feng, Yingang   +5 more
core   +1 more source

Diversity and Vertical Distribution of Planctomycetota in the Water Column of the Remote North Pacific

Environmental Microbiology Reports, Volume 17, Issue 1, February 2025.
In the remote regions of the Pacific Ocean, Planctomycetota diversity predominantly occupies deeper water layers ranging from 175 to 500 m. Phylogenetic analyses have identified an entire range of previously unknown genera within this group of bacteria.
Inês Rosado Vitorino, Nicola Gambardella, Miguel Semedo, Catarina Magalhães, Olga Maria Lage   +4 more
wiley   +1 more source

A modular enzyme with combined hemicellulose‐removing and LPMO activity increases cellulose accessibility in softwood

The FEBS Journal, Volume 292, Issue 1, Page 75-93, January 2025.
This study describes a unique bi‐catalytic enzyme that combines an oxidative activity (LPMO) acting on cellulose and a hydrolase activity (Man5) acting on mannan thus facilitating the degradation of complex, co‐polymeric plant cell walls. The enzyme works by removing the most abundant cellulose‐covering softwood hemicellulose, mannan, which then allows
Zarah Forsberg, Tina R. Tuveng, Vincent G. H. Eijsink   +2 more
wiley   +1 more source

Constructing a yeast to express the largest cellulosome complex on the cell surface

, 2022
Cellulosomes, which are multienzyme complexes from anaerobic bacteria, are considered nature’s finest cellulolytic machinery. Thus, constructing a cellulosome in an industrial yeast has long been a goal pursued by scientists.
Rizwana Parveen Rani, Wen-Hsiung Li, Marimuthu Anandharaj, Yu-Ju Lin   +3 more
core  

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source