Results 161 to 170 of about 3,121 (198)

The rosettazyme: A synthetic cellulosome

Journal of Biotechnology, 2009
Cellulose is an attractive feedstock for biofuel production because of its abundance, but the cellulose polymer is extremely stable and its constituent sugars are difficult to access. In nature, extracellular multi-enzyme complexes known as cellulosomes are among the most effective ways to transform cellulose to useable sugars.
Shigenobu, Mitsuzawa, Hiromi, Kagawa, Yifen, Li, Suzanne L, Chan, Chad D, Paavola, Jonathan D, Trent   +5 more
openaire   +2 more sources

Cellulosomes—Structure and Ultrastructure

Journal of Structural Biology, 1998
The cellulosome is a macromolecular machine, whose components interact in a synergistic manner to catalyze the efficient degradation of cellulose. The cellulosome complex is composed of numerous kinds of cellulases and related enzyme subunits, which are assembled into the complex by virtue of a unique type of scaffolding subunit (scaffoldin).
E A, Bayer, L J, Shimon, Y, Shoham, R, Lamed   +3 more
openaire   +2 more sources

Cellulose, cellulases and cellulosomes

Current Opinion in Structural Biology, 1998
The structural complexity and rigidity of cellulosic substrates have given rise to a phenomenal diversity of degradative enzymes--the cellulases. Cellulolytic microorganisms produce a wide variety of different catalytic and noncatalytic enzyme modules, which form the cellulases and act synergistically on their substrate. In some microbes, several types
Bayer, E.A., Chanzy, H., Lamed, R., Shoham, Y.   +3 more
openaire   +2 more sources

Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae

Journal of Biotechnology, 2012
We have estimated the effects of hyper-mannosylation of dockerin-type cellulase on cellulosome assembly by using Saccharomyces cerevisiae and 44 protein glycosylation mutants, because the heterologous protein displayed on yeast is assumed to be modified by yeast-specific hyper-mannosylation.
Hiroaki, Suzuki, Takao, Imaeda, Takao, Kitagawa, Katsunori, Kohda   +3 more
openaire   +2 more sources

Cellulosome and noncellulosomal cellulases of Clostridium cellulovorans

Extremophiles, 1998
This paper reviews the properties of the cellulosome and noncellulosome cellulases produced by Clostridium cellulovorans, an anaerobic, mesophilic, spore-forming microorganism that produces copious amounts of cellulase. The three major subunits of the cellulosome, CbpA, exoglucanase S (ExgS), and P100, are described, as well as the properties of the ...
Roy H Doi, Yutaka Tamaru, Akihiko Ichiishi   +2 more
exaly   +3 more sources

The cellulosome — A treasure-trove for biotechnology

Trends in Biotechnology, 1994
The cellulases of many cellulolytic bacteria are organized into discrete multienzyme complexes, called cellulosomes. The multiple subunits of cellulosomes are composed of numerous functional domains, which interact with each other and with the cellulosic substrate.
E A, Bayer, E, Morag, R, Lamed
openaire   +2 more sources

Carbohydrate Depolymerization by Intricate Cellulosomal Systems

2023
Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein-protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin ...
Sarah Moraïs, Johanna Stern, Lior Artzi, Carlos M. G. A. Fontes, Edward A. Bayer, Itzhak Mizrahi   +5 more
openaire   +2 more sources

Structure–function analyses generate novel specificities to assemble the components of multienzyme bacterial cellulosome complexes

Journal of Biological Chemistry, 2018
\ua9 2018 by The American Society for Biochemistry and Molecular Biology, Inc. The cellulosome is a remarkably intricate multienzyme nanomachine produced by anaerobic bacteria to degrade plant cell wall polysaccharides.
Pedro Bule, JOSÉ A M Prates, Luis M A Ferreira   +2 more
exaly   +1 more source

Cellulosomes: Highly Efficient Cellulolytic Complexes

2020
Cellulosomes are elaborate multienzyme complexes capable of efficiently deconstructing lignocellulosic substrates, produced by cellulolytic anaerobic microorganisms, colonizing a large variety of ecological niches. These macromolecular structures have a modular architecture and are composed of two main elements: the cohesin-bearing scaffoldins, which ...
Victor D, Alves, Carlos M G A, Fontes, Pedro, Bule   +2 more
openaire   +2 more sources

THE CELLULOSOME: The Exocellular Organelle of Clostridium

Annual Review of Microbiology, 1993
The cellulolytic enzyme complex of the anaerobic thermophile Clostridium thermocellum is reviewed. This complex, called the cellulosome, is cell associated and has a mass of from 2 x 10(6) to 6.5 x 10(6) Daltons. It consists of from 14 to 26 different polypeptides.
C R, Felix, L G, Ljungdahl
openaire   +2 more sources