Results 1 to 10 of about 208,778 (199)
Density Gradient Centrifugation of Rubella Virus [PDF]
Journal of Virology, 1968 Rubella virus was centrifuged in sucrose density gradients. One of two densities could be ascribed to the virus, depending upon the suspending medium used. The virus was found at a density of 1.16 g/cm 3 after centrifugation for 18 hr in sucrose gradients prepared in distilled water.
W. E. Rawls, Robert M. McCombs
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Sperm sexing with density gradient centrifugation in dogs
Animal Reproduction Science, 2018 Sexed sperm in dogs is of interest because of being polytocous, and as a result, the greatest number of offspring of the same sex can improve the market, although few studies assessing sperm sexing have been performed in this species. The present study, therefore, was conducted to evaluate the effects on sperm quality and the effectiveness of three ...
Gabriele Barros Mothé +7 more
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Purification of Pox Viruses by Density Gradient Centrifugation [PDF]
Journal of General Microbiology, 1962 SUMMARY: Rabbit pox virus grown in tissue culture cells and vaccinia virus grown on rabbit skin have been purified as follows. The virus was sedimented and washed once by centrifugation to provide a tenfold virus concentrate. This semi-purified material was layered on top of a sucrose density gradient and centrifuged to produce a zone of virus ...
H. T. Zwartouw +2 more
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Purification of the Scrapie Agent by Density Gradient Centrifugation
Journal of General Virology, 1984 Plasma membrane-enriched preparations from scrapie-infected and healthy hamster brains, as well as preparations of neural retina, were sonicated, then separated by rate-zonal sedimentation in 10 to 25% Nycodenz gradients. Gradient fractions were extracted with 0.5% Triton X-100 and re-fractionated by equilibrium density centrifugation in linear 25 to ...
C. Dees +4 more
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Density Gradient Centrifugation Studies of Rubella Virus
Experimental Biology and Medicine, 1966 SummaryRubella virus was grown in three different mammalian kidney cell lines. The resulting virus-containing supernates were concentrated by forced dialysis and were then studied for density on sucrose gradients. All of the virus preparations produced peaked infectivity bands with 99% of the infec-tivity concentrated in a band from 1.040 to 1.120 g ...
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Density-Gradient Centrifugation [PDF]
, 1998 In some cases, further separation of virus from the plant contaminants can be achieved by placing the clarified extract on top of a small volume of a sucrose solution in a centrifuge tube, the so-called sucrose cushion. Although the higher viscosity of the sucrose solution delays the virions reaching the bottom, the plant contaminants may be slowed ...
Jeanne Dijkstra, Cees P. de Jager
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Centrifugation of brain microsomes in a density gradient
Experimental Cell Research, 1960Abstract Microsomal preparations from rat brain were centrifuged in various density gradients obtained with different concentrations of sucrose. The different zones of the gradients were analyzed chemically as to their protein and RNA content and examined with the electron microscope as to their content of particles and membrane structures.
G. Toschi, V. Hanzon
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The Effects of Overloading in Density-Gradient Centrifugation
European Journal of Biochemistry, 1975The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
Lars Funding +2 more
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Zonal centrifugation in reorienting density gradients
Biochimica et Biophysica Acta (BBA) - General Subjects, 1971Abstract 1. 1. Zonal rotors which do not require rotating-seal assemblies were tested and employed for quantitative cell fractionations in sucrose density gradients. The design and operation of these rotors represents a departure from other zonal rotors presently in use.
John R. Wells +2 more
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Separation of nanorods by density gradient centrifugation
Journal of Chromatography A, 2011The experimental conditions necessary for the synthesis of well-defined nanoparticles are often difficult to control. There is thus a compelling need for post-synthesis separation of nanoparticles polydispersed in size and shape. We demonstrate here both theoretically and experimentally that gold nanorods with diverse aspect ratios can be separated ...
Yan He +5 more
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