Results 1 to 10 of about 380,843 (313)

Protocol to isolate quiescent hepatic stellate cells in sufficient yield while maintaining cytokine responsiveness from aged female mice [PDF]

STAR Protocols
Summary: Isolation of primary hepatic stellate cells (HSCs) in sufficient quantities is technically challenging. We present a protocol for isolating quiescent HSCs from mice using Nycodenz density-gradient centrifugation.
Takao Seki, Sachiko Komazawa-Sakon, Yuichi Tsuchiya, Kyoko Fukuhara, Yasuhiro Nakano, Kenichi Ikejima, Katsuhide Okunishi, Hiroyasu Nakano   +7 more
doaj   +2 more sources

Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90%

MethodsX, 2021
Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa, Andrea Galuppo, Rômulo Batista Rodrigues, Nathalia dos Santos Teixeira, Thaiza Rodrigues de Freitas, Thales de Souza França, Lis Santos Marques, Itamar Cossina Gomes, Danilo Pedro Streit, Jr.   +8 more
doaj   +1 more source

Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

MethodsX, 2023
Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay, Rashmi Ravindran Nair, Deepti Sharan, Kishor Jakkala, Parthasarathi Ajitkumar   +4 more
doaj   +1 more source

#353 : Microfluidic Preparation of Spermatozoa: A Potential Approach in Assisted Reproductive Technology

Fertility & Reproduction, 2023
Background and Aims: While density gradient centrifugation (DGC) is a conventional method in sperm preparation, this method can induce reactive oxygen species (ROS) which cause sperm dysfunction, sperm DNA damage, a recent advancement in sperm processing
Nguyet Minh Ho, Thuy Huong Do, Sy Hung Ho, Manh Ha Nguyen   +3 more
doaj   +1 more source

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]

Veterinary World, 2022
Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles, Imam Mustofa, Mas'ud Hariadi, Wurlina Wurlina, Suherni Susilowati, Anny Amaliya, Suparto Suparto, Rimayanti Rimayanti   +7 more
doaj   +1 more source

Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial

Life, 2022
The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated.
Usha Punjabi, Ella Roelant, Kris Peeters, Ilse Goovaerts, Helga Van Mulders, Diane De Neubourg   +5 more
doaj   +1 more source

Density gradient centrifugation compromises bone marrow mononuclear cell yield. [PDF]

PLoS ONE, 2012
Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft ...
Claudia Pösel, Karoline Möller, Wenke Fröhlich, Isabell Schulz, Johannes Boltze, Daniel-Christoph Wagner   +5 more
doaj   +1 more source

Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

Frontiers in Immunology, 2021
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz, Manuel Obermaier, Sandra Uhle, Markus Alexander Weigand, Florian Uhle   +4 more
doaj   +1 more source

Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]

International Journal of Fertility and Sterility, 2020
Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini, Tayebeh Izadi, Marziyeh Tavalaee, Leila Azadi, Mehdi Hajian, Mahshid Rahimi Zamani, Mohammad Hossein Nasr-Esfahani   +6 more
doaj   +1 more source

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

Viruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan   +3 more
doaj   +1 more source