Results 21 to 30 of about 229,025 (265)

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu   +3 more
doaj   +1 more source

Elimination of bacteria from semen using a combination of density gradient centrifugation and swim-up

Journal of Men's Health, 2022
Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun   +6 more
doaj   +1 more source

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +1 more source

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing

Reproductive Medicine and Biology
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao   +15 more
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Fractionating microplastics by density gradient centrifugation: a novel approach using LuerLock syringes in a low-cost density gradient maker

Chemistry Teacher International
Microplastics are now ubiquitous in the environment and are even considered “technofossils” of the Anthropocene. Given their omnipresence and potential impact, identifying and analyzing these particles becomes increasingly crucial.
Majcen Alina, Gohla Jan, Steinhoff Anna S., Meißner Lena, Tassoti Sebastian, Spitzer Philipp   +5 more
doaj   +1 more source

IMPACT OF PREPARATION USING CONVENTIONAL AND MODIFIED DENSITY GRADIENT CENTRIFUGATION METHODS ON SPERM CONCENTRATION, MOTILITY AND NUMBER OF NORMAL MOTILE SPERM RECOVERY (NMSR)

Folia Medica Indonesiana, 2017
Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques.
Rina Yudiwati, MPBD Pramesti, Agustinus Agustinus, E Pradana, Bambang Purwanto   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source