Results 41 to 50 of about 380,843 (313)

Fractionating microplastics by density gradient centrifugation: a novel approach using LuerLock syringes in a low-cost density gradient maker

Chemistry Teacher International
Microplastics are now ubiquitous in the environment and are even considered “technofossils” of the Anthropocene. Given their omnipresence and potential impact, identifying and analyzing these particles becomes increasingly crucial.
Majcen Alina, Gohla Jan, Steinhoff Anna S., Meißner Lena, Tassoti Sebastian, Spitzer Philipp   +5 more
doaj   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation

Nanomaterials, 2016
Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors.
Monika Mortimer, Elijah J. Petersen, Bruce A. Buchholz, Patricia A. Holden   +3 more
doaj   +1 more source

A density gradient centrifugation method for rapid separation of nanoTiO2 and TiO2 aggregates from microalgal cells in complex mixtures with mercury

MethodsX, 2020
In natural environment, the microorganisms are exposed to complex mixtures of contaminants, including manufactured nanoparticles and their aggregates.
Mengting Li, Vera I. Slaveykova
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Bio-Protocol, 2015
Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991).
Jorge Verdín, Eddy Sánchez-León, Rosa Fajardo-Somera, Carlos Morales, Salomón Bartnicki-García, Meritxell Riquelme   +5 more
doaj   +1 more source

Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation

The World Journal of Men's Health, 2021
Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated.
Gabriela Hernández-Silva, Aideé S. López-Torres, Israel Maldonado-Rosas, Esperanza Mata-Martínez, Fernando Larrea, Víctor Torres-Flores, Claudia L. Treviño, Mayel Chirinos   +7 more
doaj   +1 more source