Results 111 to 120 of about 1,582,967 (422)

Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new BD-Barricor tubes

open access: yesBiochemia Medica, 2017
Introduction Although centrifugation is performed in almost every blood sample, recommendations on duration and g-force are heterogeneous and mostly based on expert opinions.
J. Cadamuro   +8 more
semanticscholar   +1 more source

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

open access: yesFEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg   +2 more
wiley   +1 more source

Application of Filtration to Recover Solubilized Proteins During pH-Shift Processing of Blue Whiting (Micromesistius poutassou); Effects on Protein Yield and Qualities of Protein Isolates [PDF]

open access: yes, 2011
Previous studies of the pH-shift protein isolation process have shown that substantial amounts of solubilized proteins can be trapped in the sediments formed in the first centrifugation step of this process.
Marmon, Sofia   +2 more
core   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

open access: yesFEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt   +4 more
wiley   +1 more source

Improved extraction technique for biological fluids [PDF]

open access: yes, 1975
Liquid-liquid extraction technique speeds up separation of biological fluids into number of compounds. This eliminates agitation, emulsion formation, centrifugation, mechanical separation of phases, filtration, and other steps that have been used ...
Jahnsen, V. J.
core   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

open access: yesFEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche   +2 more
wiley   +1 more source

An assessment of a new synthetic procedure for core-shell polymeric supports based on the Amberlite XAD-4 adsorbent

open access: yesActa Chimica Slovenica, 2015
In pursuit for new reactive materials designed for synthesis of functional resins, the novel core-shell type polymeric supports with accessible chloromethyl groups were synthesized.
Piotr Cyganowski   +3 more
doaj   +1 more source

Centrifugal force reversal from the perspective of rigidly rotating observer [PDF]

open access: yesarXiv, 2011
In previous studies the dynamics of the relativistic particle moving along the rotating pipe was investigated. The simple gedanken experiment was considered. It was shown that at large enough velocities a centrifugal force acting on the bead changes its usual sign and attracts towards the rotation axis.
arxiv  

Flow‐based immunomagnetic enrichment of circulating tumor cells from diagnostic leukapheresis product

open access: yesMolecular Oncology, EarlyView.
The number of circulating tumor cells obtained from prostate cancer patients was increased approximately 5‐fold compared to regular CellSearch when processing 2 mL diagnostic leukapheresis material aliquots and increased by 44‐fold when processing 20 mL DLA aliquots using the flow enrichment target capture Halbach‐array.
Michiel Stevens   +8 more
wiley   +1 more source

Effect of Separating Giant Foxtail (Setaria faberi) Seeds from Soil Using Potassium Carbonate and Centrifugation on Viability and Germination [PDF]

open access: yes, 1998
Changes in weed seedbank composition are often monitored by removing seeds from soil samples. One extraction method accomplishes this by creating a slurry of soil and a concentrated inorganic salt solution.
Buhler, Douglas   +3 more
core   +2 more sources

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