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Redefining the role of the transfusion medicine physician in the era of advanced cellular therapies

open access: yes
Transfusion, Volume 66, Issue 8, Page 1645-1656, August 2026.
Eric A. Gehrie, Kevin J. Land
wiley   +1 more source
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Specific cGMP binding by the cGMP binding domains of cGMP-binding cGMP specific phosphodiesterase

Cellular Signalling, 2002
The structure of cyclic GMP (cGMP)-binding (cGB), cGMP specific phosphodiesterase (PDE5) comprises several domains. We have used RT-PCR methods to clone the noncatalytic cGB domains of PDE5 from human colon cancer cell RNA and constructed glutathione-S-transferase (GST) fusion proteins to express and study the domains.
Rifat Pamukçu   +2 more
exaly   +3 more sources

A primer for measuring cGMP signaling and cGMP-mediated vascular relaxation

Nitric Oxide, 2021
Soluble guanylyl cyclase (sGC, also called GC1) is the main receptor for nitric oxide (NO) that catalyzes the production of the second messenger molecule, 3'5' cyclic guanosine monophosphate (cGMP) leading to vasorelaxation, and inhibition of leukocyte recruitment and platelet aggregation.
Adam C. Straub, Annie Beuve
openaire   +2 more sources

Allosteric Activation of cGMP-Specific, cGMP-Binding Phosphodiesterase (PDE5) by cGMP

Biochemistry, 2002
The effects of cGMP binding on the catalytic activity of cGMP-specific, cGMP-binding phosphodiesterase (PDE5) are unclear because cGMP interacts with both allosteric and catalytic sites specifically. We studied the effects of cGMP on the hydrolysis of a fluorescent substrate analogue, 2'-O-anthraniloyl cGMP, by PDE5 partially purified from rat ...
Daisuke, Okada, Shigeki, Asakawa
openaire   +2 more sources

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