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Chalcone synthase and its functions in plant resistance [PDF]

Phytochemistry Reviews, 2011
Chalcone synthase (CHS, EC 2.3.1.74) is a key enzyme of the flavonoid/isoflavonoid biosynthesis pathway. Besides being part of the plant developmental program the CHS gene expression is induced in plants under stress conditions such as UV light, bacterial or fungal infection. CHS expression causes accumulation of flavonoid and isoflavonoid phytoalexins
Robert Verpoorte, Verpoorte R
exaly   +3 more sources

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Improved preparation and assay of chalcone synthase

Phytochemistry, 1985
Abstract Preparation and assay of chalcone synthase in presence of sodium ascorbate and exclusion of oxygen during some steps gives improved yield and purity of 2 S -naringenin.
Hans Grisebach, Lothar Britsch
exaly   +2 more sources

Chalcone synthase in the liverwort Marchantia polymorpha

Phytochemistry, 1995
Chalcone synthase (CHS) activity was measured in gametophytic tissue of Marchantia polymorpha. The main product of the enzyme reaction was identified as naringenin which results from cyclization of the chalcone. Cross-reactivity under native and denaturing conditions was obtained using antibodies raised against CHS from three different higher plants ...
Gottfried Weissenböck
exaly   +2 more sources

Molecular evolution and sequence divergence of plant chalcone synthase and chalcone synthase-Like genes

Genetica, 2014
Plant chalcone synthase (CHS) and CHS-Like (CHSL) proteins are polyketide synthases. In this study, we evaluated the molecular evolution of this gene family using representative types of CHSL genes, including stilbene synthase (STS), 2-pyrone synthase (2-PS), bibenzyl synthase (BBS), acridone synthase (ACS), biphenyl synthase (BIS), benzalacetone ...
Yingying, Han, Wenwen, Zhao, Zhicui, Wang, Jingying, Zhu, Qisong, Liu   +4 more
openaire   +2 more sources

The Chalcone Synthase Superfamily of Type III Polyketide Synthases

ChemInform, 2002
AbstractFor Abstract see ChemInform Abstract in Full Text.
Michael B, Austin, Joseph P, Noel
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Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings

Plant Molecular Biology, 1997
Antibodies have been developed against the first two enzymes of flavonoid biosynthesis in Arabidopsis thaliana. Chalcone synthase (CHS) and chalcone isomerase (CHI) were overexpressed and purified from Escherichia coli as fusion proteins with glutathione S-transferase from Schistosoma japonicum.
C C, Cain, D E, Saslowsky, R A, Walker, B W, Shirley   +3 more
openaire   +2 more sources

EXPRESSION OF CHALCONE SYNTHASE GENE IN WALNUT

Acta Horticulturae, 1994
A rapid and efficient method has been succesfully applied to the isolation and purification of RNA from lyophilised tissues of walnut trees (Juglans sp.) particularly rich in phenolic compounds and polysaccharides. The quality of the extracted RNA has been characterized by gel electrophoresis and Northern blot hybridization.
Claudot, A.-C., Jouanin, L., Ernst, D., Sandermann, H., Drouet, A.   +4 more
openaire   +2 more sources

Chalcone synthases (CHSs): the symbolic type III polyketide synthases

Planta, 2019
Present review provides a thorough insight on some significant aspects of CHSs over a period of about past three decades with a better outlook for future studies toward comprehending the structural and mechanistic intricacy of this symbolic enzyme. Polyketide synthases (PKSs) form a large family of iteratively acting multifunctional proteins that are ...
Shahzad A, Pandith, Salika, Ramazan, Mohd Ishfaq, Khan, Zafar A, Reshi, Manzoor A, Shah   +4 more
openaire   +2 more sources

An allelic series for the chalcone synthase locus in Arabidopsis

Gene, 2000
Five new alleles of the Arabidopsis chalcone synthase (CHS) locus, tt4, have been characterized at the gene, protein, and end product levels as a genetic approach to understanding structure-function relationships in a key enzyme of plant secondary metabolism.
D E, Saslowsky, C D, Dana, B, Winkel-Shirley   +2 more
openaire   +2 more sources

A chalcone synthase/stilbene synthase DNA probe for conifers

Theoretical and Applied Genetics, 1996
A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P.
S M, Baker, E E, White
exaly   +3 more sources