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Optimum chalcone synthase for flavonoid biosynthesis in microorganisms

Critical Reviews in Biotechnology, 2021
Chalcones and the subsequently generated flavonoids, as well as flavonoid derivatives, have been proven to have a variety of physiological activities and are widely used in: the pharmaceutical, food, feed, and cosmetic industries. As the content of chalcones and downstream products in native plants is low, the production of these compounds by ...
Yingjia, Tong   +4 more
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Molecular evolution and sequence divergence of plant chalcone synthase and chalcone synthase-Like genes

Genetica, 2014
Plant chalcone synthase (CHS) and CHS-Like (CHSL) proteins are polyketide synthases. In this study, we evaluated the molecular evolution of this gene family using representative types of CHSL genes, including stilbene synthase (STS), 2-pyrone synthase (2-PS), bibenzyl synthase (BBS), acridone synthase (ACS), biphenyl synthase (BIS), benzalacetone ...
Yingying, Han   +4 more
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The Chalcone Synthase Superfamily of Type III Polyketide Synthases

ChemInform, 2002
AbstractFor Abstract see ChemInform Abstract in Full Text.
Michael B, Austin, Joseph P, Noel
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Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings

Plant Molecular Biology, 1997
Antibodies have been developed against the first two enzymes of flavonoid biosynthesis in Arabidopsis thaliana. Chalcone synthase (CHS) and chalcone isomerase (CHI) were overexpressed and purified from Escherichia coli as fusion proteins with glutathione S-transferase from Schistosoma japonicum.
C C, Cain   +3 more
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Chalcone synthases (CHSs): the symbolic type III polyketide synthases

Planta, 2019
Present review provides a thorough insight on some significant aspects of CHSs over a period of about past three decades with a better outlook for future studies toward comprehending the structural and mechanistic intricacy of this symbolic enzyme. Polyketide synthases (PKSs) form a large family of iteratively acting multifunctional proteins that are ...
Shahzad A, Pandith   +4 more
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A chalcone synthase/stilbene synthase DNA probe for conifers

Theoretical and Applied Genetics, 1996
A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P.
S M, Baker, E E, White
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EXPRESSION OF CHALCONE SYNTHASE GENE IN WALNUT

Acta Horticulturae, 1994
A rapid and efficient method has been succesfully applied to the isolation and purification of RNA from lyophilised tissues of walnut trees (Juglans sp.) particularly rich in phenolic compounds and polysaccharides. The quality of the extracted RNA has been characterized by gel electrophoresis and Northern blot hybridization.
Claudot, A.-C.   +4 more
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Grapevine clones discriminated using stilbene synthase–chalcone synthase markers

Journal of the Science of Food and Agriculture, 2004
AbstractTouriga Nacional is the most popular Portuguese grapevine cultivar. After twenty years of clonal selection, highly recommended qualitative and productive clones have been identified. In the genus Vitis several molecular markers have been used to characterize genetic diversity at the DNA level, namely microsatellites, ISSR (inter simple sequence
Miguel A Faria   +4 more
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