Results 181 to 190 of about 12,872 (224)
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Improved preparation and assay of chalcone synthase
Phytochemistry, 1985Abstract Preparation and assay of chalcone synthase in presence of sodium ascorbate and exclusion of oxygen during some steps gives improved yield and purity of 2 S -naringenin.
Lothar Britsch, Hans Grisebach
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Characterization of Antisense Chalcone Synthase Transgenic Microcuttings
1996Walnut somatic embryos were transformed with a vector containing the neomycin phosphotransferase (nptII), b-glucuronidase (uidA) and an antisense chalcone synthase (chs) genes. Thirteen transgenic lines were obtained. They were characterized at the molecular, biochemical, and physiological levels, and compared to control lines (the initial line and a ...
Jouanin, Lise +5 more
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Engineered Biosynthesis of Plant Polyketides: Manipulation of Chalcone Synthase
Organic Letters, 2006[reaction: see text]. Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA.
Ikuro, Abe +4 more
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Enzymatic Formation of Unnatural Aromatic Polyketides by Chalcone Synthase
Biochemical and Biophysical Research Communications, 2000Substrate specificity of recombinant chalcone synthase (CHS) from Scutellaria baicalensis (Labiatae) was investigated using chemically synthesized aromatic and aliphatic CoA esters. It was demonstrated for the first time that CHS converted benzoyl-CoA to phlorobenzophenone (2,4,6-trihydroxybenzophenone) along with pyrone by-products. On the other hand,
H, Morita +3 more
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Chalcone synthase in the liverwort Marchantia polymorpha
Phytochemistry, 1995Chalcone synthase (CHS) activity was measured in gametophytic tissue of Marchantia polymorpha. The main product of the enzyme reaction was identified as naringenin which results from cyclization of the chalcone. Cross-reactivity under native and denaturing conditions was obtained using antibodies raised against CHS from three different higher plants ...
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Chalcone synthases from spinach (Spinacia oleracea L.)
Planta, 1988The distribution of the two chalcone synthases in leaves ofSpinacia oleracea L. was studied at both the tissue and the subcellular level using immunofluorescence and immunogold techniques. Neither technique differentiated between the two enzyme forms. The chalcone synthases are located in the upper and the lower epidermis and to a minor extent in the ...
L, Beerhues, H, Robenek, R, Wiermann
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Structure, Function, and Regulation of the Chalcone Synthase
1993Publisher Summary This chapter presents an overview of the activity, function, and control of chalcone synthase (CHS) enzyme. It describes the reaction it catalyzes, the secondary metabolic products of the pathways in which it is involved, and the functions of these products.
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CLONING OF DIVERGENT CHALCONE SYNTHASE SEQUENCES FROM PELARGONIUM
Acta Horticulturae, 2004Chalcone synthase (CHS) is the key enzyme of the flavonoid-anthocyanin pathway. It leads to the production of a highly diverse group of compounds. Its members play different roles in the interaction of the plant with its environment. In most studied plants, not only one gene, but a small gene family codes for CHS-like enzymes.
L. Jánváry, G. Forkmann
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Chalcone synthase from cell suspension cultures of Daucus carota L
Archives of Biochemistry and Biophysics, 1985Chalcone synthase (CHS) has been partially purified about 35-fold. Withdrawal of 2-mercaptoethanol after precipitation with ammonium sulfate led to higher stability during further purification steps. In order to determine CHS activity, two procedures [according to Schröder et al. (1979) Plant Sci. Lett. 14, 281-286] were applied.
W, Hinderer, H U, Seitz
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Molecular characterization of root-specific chalcone synthases from Cassia alata
Planta, 2002Three cDNAs encoding very similar but unique isoforms of chalcone synthase (EC 2.3.1.74) were isolated from a cDNA library prepared from RNA from root tissue of the Thai medicinal plant Cassia alata L. (ringworm bush, Leguminosae). Gene transcript for these three type-III polyketide synthases was found to accumulate predominantly in roots.
Supachai, Samappito +4 more
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