Results 161 to 170 of about 28,092 (189)

Cation-Mediated Interplay of Loops in Chaperonin-10

Journal of Biomolecular Structure and Dynamics, 2006
The ubiquitously occurring chaperonins consist of a large tetradecameric Chaperonin-60, forming a cylindrical assembly, and a smaller heptameric Chaperonin-10. For a functional protein folding cycle, Chaperonin-10 caps the cylindrical Chaperonin-60 from one end forming an asymmetric complex.
Swetha, Vijayakrishnan, Rohini, Qamra, Chandra S, Verma, Ranjan, Sen, Shekhar C, Mande   +4 more
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Overproduction and purification of Mycobacterium tuberculosis chaperonin 10

Gene, 1994
The overexpression of the Mycobacterium tuberculosis chaperonin 10 (Cpn10)-encoding gene was accomplished using baculovirus expression vectors. The product was immunoreactive with a Cpn10 monoclonal antibody (mAb) and had an electrophoretic mobility identical to authentic Cpn10. The baculovirus system was most successful in terms of reaching nearly the
D, Atkins, H, al-Ghusein, C, Prehaud, A R, Coates   +3 more
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Identification and cloning of human chaperonin 10 homologue

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994
We have identified a heat-shock-inducible 10 kDa protein in the human hepatoma cell line HepG2. The total RNA extracted from the heat-shocked cells was amplified by reverse transcription PCR (polymerase chain reaction) using 21 5' and 18 3' oligonucleotides of rat cpn10 (chaperonin10) cDNA as primers.
Monzini N, Legname, Giuseppe, Marcucci F, Gromo G, Modena D.   +4 more
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Purification and characterization of the chaperonin 10 and chaperonin 60 proteins from Rhodobacter sphaeroides

Biochemistry, 1991
Two heat-shock proteins that show high identity with the Escherichia coli chaperonin 60 (groEL) and chaperonin 10 (groES) chaperonin proteins were purified and characterized from photolithoautotrophically grown Rhodobacter sphaeroides. The proteins were purified by using sucrose density gradient centrifugation and Mono-Q anion-exchange chromatography ...
K C, Terlesky, F R, Tabita
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The Human Early Pregnancy Factor/Chaperonin 10 Gene Family

Biochemical and Molecular Medicine, 1996
cDNA clones corresponding to the sequence for human early pregnancy factor were isolated from a human melanoma library and hybridized to DNA digested with four restriction enzymes obtained from twelve different subjects. Up to 20 cross hybridizing bands were observed.
Summers, K. M., Murphy, R. M., Webb, G. C., Peters, G. B., Morton, H., Cassady, A. I., Cavanagh, A. C.   +6 more
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Purification and characterization of chaperonin 10 from Chromatium vinosum

Archives of Biochemistry and Biophysics, 1992
Chromatium vinosum contains a polypeptide that is functionally and structurally similar to the Escherichia coli chaperonin 10. The protein has been purified to homogeneity by sucrose density gradient centrifugation followed by gel filtration using a Bio-Gel A-1.5 m column.
J A, Torres-Ruiz, B A, McFadden
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Arabidopsis Chloroplast Chaperonin 10 Is a Calmodulin-Binding Protein

Biochemical and Biophysical Research Communications, 2000
Calcium regulates diverse cellular activities in plants through the action of calmodulin (CaM). By using (35)S-labeled CaM to screen an Arabidopsis seedling cDNA expression library, a cDNA designated as AtCh-CPN10 (Arabidopsis thaliana chloroplast chaperonin 10) was cloned.
T, Yang, B W, Poovaiah
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Purification and characterization of chaperonin 60 and chaperonin 10 from the anaerobic thermophile Thermoanaerobacter brockii

European Journal of Biochemistry, 1994
Chaperonin 60 and chaperonin 10 (GroEL and GroES homologues, respectively) have been isolated from extracts of the anaerobic thermophile Thermoanaerobacter brockii. A simple and rapid purification for chaperonin 60 made use of hydrophobic and anion‐exchange chromatographies, and could be readily scaled up; approximately 2 mg pure chaperonin 60 was ...
Truscott, KN, Hoj, PB, Scopes, RK
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The Mycobacterium tuberculosis chaperonin 10 monomer exhibits structural plasticity

Biopolymers, 2004
AbstractThe conditions which favor dissociation of oligomeric Mycobacterium tuberculosis chaperonin 10 and the solution structure of the monomer were studied by analytical ultracentrifugation, size exclusion chromatography, fluorescence, and circular dichroism spectroscopies.
Gianluca, Fossati, Pietro, Cremonesi, Gaetano, Izzo, Emanuele, Rizzi, Giovanni, Sandrone, Stephen, Harding, Neil, Errington, Christopher, Walters, Brian, Henderson, Michael M, Roberts, Anthony R M, Coates, Paolo, Mascagni   +11 more
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Preparation and characterization of polyclonal antibodies against human chaperonin 10

Cell Stress & Chaperones, 2000
Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein ...
Somodevilla-Torres, Maria J., Hillyard, Narelle C., Morton, Halle, Alewood, Dianne, Halliday, Judy A., Alewood, Paul F., Vesey, David A., Walsh, Michael D., Cavanagh, Alice C.   +8 more
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