B-cell epitopes in GroEL of Francisella tularensis. [PDF]
The chaperonin protein GroEL, also known as heat shock protein 60 (Hsp60), is a prominent antigen in the human and mouse antibody response to the facultative intracellular bacterium Francisella tularensis (Ft), the causative agent of tularemia.
Zhaohua Lu +13 more
doaj +1 more source
IntroductionMastitis is a widespread mammary gland disease of dairy cows that causes severe economic losses to dairy farms. Mastitis can be caused by bacteria, fungi, and algae.
Anna Dobrut +7 more
doaj +1 more source
Effects of confinement and crowding on folding of model proteins
We perform molecular dynamics simulations for a simple coarse-grained model of crambin placed inside of a softly repulsive sphere of radius R. The confinement makes folding at the optimal temperature slower and affects the folding scenarios, but both ...
Baruah +26 more
core +1 more source
Assessment of plant chaperonin-60 gene function in Escherichia coli
Brassica napus chaperonin-60 alpha and chaperonin-60 beta genes expressed separately and in combination produce three novel Escherichia coli strains: alpha, beta, and alpha beta. In beta and alpha beta cells, the plant gene products assemble efficiently into tetradecameric cpn60(14) species, including novel hybrids containing both bacterial and plant ...
L P Cloney +3 more
openaire +3 more sources
ABSTRACT Clostridioides difficile (CD) is a leading cause of antibiotic‐associated diarrhoea in both hospitalized and non‐hospitalized patients. This study explores the role of extracellular vesicles (EVs) derived from Clostridioides difficile strain 630 (CD630‐EVs), which released spherical EVs during in vitro culture, in modulating pro‐inflammatory ...
Fangfang Fan +11 more
wiley +1 more source
Transcriptomic and proteomic profiles of II YOU 838 (Oryza sativa) provide insights into heat stress tolerance in hybrid rice [PDF]
Heat stress is an increasing threat to rice production worldwide. To investigate the mechanisms of heat tolerance in hybrid rice and their contributions to rice heterosis, we compared the transcriptome of the hybrid rice II YOU 838 (II8) with the ...
Yan Wang +8 more
doaj +2 more sources
Acetyl‐Phosphate Dependent Protein Acetylation in Neisseria gonorrhoeae
The role of acetyl‐phosphate‐dependent non‐enzymatic protein acetylation in Neisseria gonorrhoeae was investigated through the deletion of the phosphotransacetylase (pta) and the acetate kinase (ackA) genes, this led to a decrease and increase in protein acetylation, respectively.
Ernesto F. D. Parga +6 more
wiley +1 more source
Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit
In this study, we performed the first comparative proteomic analysis of wheat flag leaves and developing grains in response to drought stress. Drought stress caused a significant decrease in several important physiological and biochemical parameters and ...
Xiong Deng +7 more
doaj +1 more source
Quantitative molecular diagnostic assays of grain washes for Claviceps purpurea are correlated with visual determinations of ergot contamination. [PDF]
We examined the epiphytic microbiome of cereal grain using the universal barcode chaperonin-60 (cpn60). Microbial community profiling of seed washes containing DNA extracts prepared from field-grown cereal grain detected sequences from a fungus ...
Alexia Comte +4 more
doaj +1 more source
A yeast two-hybrid screen reveals a strong interaction between the Legionella chaperonin Hsp60 and the host cell small heat shock protein Hsp10 [PDF]
L. pneumophila is an intracellular bacterium that replicates inside a membrane-bound vacuole called Legionella-containing vacuole (LCV), where it plentifully liberates its HtpB chaperonin.
Nasrallah, Gheyath K.
core +1 more source

