Results 111 to 120 of about 912,461 (203)

Adrenal venous sampling criteria for chemiluminescent enzyme immunoassay as a preferable alternative to radioimmunoassay in primary aldosteronism

Endocrine Journal
Plasma aldosterone concentration (PAC) was routinely measured using radioimmunoassay (RIA); however, the RIA kit was discontinued in March 2021 in Japan.
Kazuki Nakai, Yuya Tsurutani, Koki Irie, Kyoko Teruyama, Sachiko Suematsu, Seishi Matsui, Kohzoh Makita, Jun Saito, Masao Omura, Tetsuo Nishikawa   +9 more
doaj   +1 more source

Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis. [PDF]

J Infect Chemother, 2021
Ishii T, Sasaki M, Yamada K, Kato D, Osuka H, Aoki K, Morita T, Ishii Y, Tateda K.   +8 more
europepmc   +1 more source

Advances in Viral Markers for Predicting Functional Cure of Chronic Hepatitis B

Portal Hypertension &Cirrhosis, EarlyView.
Functional cure, defined as sustained hepatitis B surface antigen (HBsAg) loss, is achieved by only a minority of patients with chronic hepatitis B (CHB); early identification of likely responders may improve cure rates and reduce costs. However, responses to antiviral therapy vary considerably, necessitating reliable hepatitis B virus (HBV) viral ...
Xinrong Lu, Xin Yang, Qishui Ou, Zhen Xun   +3 more
wiley   +1 more source

Carbon dots in electrochemiluminescence: Mechanisms, interface engineering, and bioanalytical applications

Responsive Materials, EarlyView.
Carbon dots are emerging as versatile electrochemiluminescent nanomaterials whose structure, surface states, and interfacial design jointly determine ECL pathways and performance. This review highlights how mechanistic understanding and materials engineering enable programmable CD‐ECL systems for bioanalysis, imaging, and future intelligent analytical ...
Yuhan Zhao, Zixuan Chen, Jun‐Jie Zhu
wiley   +1 more source

Enzyme immunoassay for cortisol in serum using cortisol 21-amine

, 1978
Cortisol 21-amine (21-amino-11β,17-dihydroxy-4-pregnene-3,20-dione) was prepared and an enzyme immunoassay for cortisol in serum was established using cortisol 21-amine conjugated with alkaline phosphatase. The minimal amount of cortisol detected was 1ng/
Toshio, Ogihara, Fukuko, Watanabe, Yuichi, Kumahara, Yoshiharu, Kobayashi, Ichiya, Ninomiya, Kiyoko, Amitani, Toshiko, Kiguchi   +6 more
core   +1 more source

Rapid and Efficient Affinity Isolation of Engineered Extracellular Vesicles Encapsulating Functional Proteins via Internal Twin‐Strep Tetraspanin Tagging

Small, EarlyView.
Structure‐guided internal twin‐Strep tagging of the tetraspanins CD63 or CD9 enables rapid and efficient antibody‐free affinity isolation of engineered extracellular vesicles (EVs) using Strep technology while allowing luminal protein loading. The approach removes over 96% of contaminants, separates tagged EVs from coexisting viral particles, and ...
Minh‐Tu Pham, Jona Benjamin Krohn, Janis Meyer, Christian Ritter, Martin Schneider, Dominic Helm, Dario Frey, Kleopatra Rapti, Uta Haselmann‐Weiss, Elif Toprak, Viet Loan Dao Thi, Florian Leuschner, Dirk Grimm, Karl Rohr, Ralf Bartenschlager   +14 more
wiley   +1 more source

Electron‐Transfer Engineered Biointerface With Detonation Nanodiamonds for Next‐Generation Cortisol Electrochemical Immunoassay

Small, EarlyView.
Screen‐printed carbon electrode biointerface featuring detonation nanodiamond layer with the sp2/sp3 carbon interface that optimizes charge transfer and enables covalent immobilization of cortisol antibodies. The designed electrochemical sensor provides high selectivity, stability and rapid cortisol detection also from saliva samples.
Chakavak Esmaeili, Jaroslav Kuliček, Markéta Šlapal Bařinková, Bohuslav Rezek   +3 more
wiley   +1 more source

Engineering Carbon Nanotube Quantum Well Defects With Recognition Tripeptides for Optical Detection of Extracellular Vesicles in Plasma

Small, EarlyView.
A three‐residue peptide bioconjugated onto a carbon nanotube quantum defect turns the near‐infrared emission of quantum defects into a reporter of integrins on intact extracellular vesicles, directly in human plasma. The sensor reveals that healthy fibroblast vesicles carry as much RGD‐binding integrin as cancer‐derived ones, an adhesion‐associated ...
In‐Jun Hwang, Junhee Kim, Aarti Patel, Leilei Zhang, Jacob Miller, Stanislav Piletsky, Cassandra L. Clift, Colin Hisey, YongJoo Kim, Mijin Kim   +9 more
wiley   +1 more source

Synergistic Toxicity of Low‐Concentration Metal Mixture on Male Rats: Reproductive, Renal, and Hepatic Effects

Environmental Toxicology, EarlyView.
ABSTRACT Mining plays a crucial role in economic development, but improper management can lead to severe environmental degradation. Particularly for iron ore, mining generates vast amounts of tailings often stored in unstable dams. This study aims to investigate the toxicological impacts of environmentally relevant concentrations of metals mixture ...
Kalinka Helóra Gomes de Almeida, Thalita Tofanin Sargiani D'Assumpção, Ana Priscila Gomes Silva, Carolina Rocha Moreira de Oliveira, Tailisi Hoppe Trevizani, Rubens Cesar Lopes Figueira, Lucas Buruaem Moreira, Juliana Elaine Perobelli   +7 more
wiley   +1 more source

Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay. [PDF]

J Infect Chemother, 2022
Kato H, Miyakawa K, Ohtake N, Go H, Yamaoka Y, Yajima S, Shimada T, Goto A, Nakajima H, Ryo A.   +9 more
europepmc   +1 more source