A physiological study of chick myotubes grown in tissue culture
1. A study has been made of some passive and active membrane properties of myotubes of different ages obtained in culture from explants of chick embryo thigh muscle.2. After 3 days in vitro the mean values for the myotube resting membrane potential and input resistance were ‐ 63·8 mV and 1·30 MΩ respectively.
J B, Harris, M W, Marshall, P, Wilson
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cDNA that encodes active agrin [PDF]
Agrin is thought to mediate the motor neuron-induced aggregation of AChRs and AChE on the surface of muscle fibers at neuromuscular junctions. We have isolated a cDNA from a chick brain library that, based on sequence homology and expression experiments,
Bolander +42 more
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Culture conditions influence satellite cell activation and survival of single myofibers [PDF]
Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved.
Adamo, Sergio +5 more
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The agrin gene codes for a family of basal lamina proteins that differ in function and distribution [PDF]
We isolated two cDNAs that encode isoforms of agrin, the basal lamina protein that mediates the motor neuron-induced aggregation of acetylcholine receptors on muscle fibers at the neuromuscular junction.
Escher, G. +6 more
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Isolation of a new high molecular weight protein associated with desmin and vimentin filaments from avian embryonic skeletal muscle [PDF]
Filaments with a diameter of 80-120 Å have been prepared from 14-d-old chick embryonic skeletal muscle, using a physiological salt solution and gel filtration chromatography.
Breckler, Jennifer, Lazarides, Elias
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Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]
Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW +3 more
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Enhanced chemosensitivity of chick parasympathetic neurones in co‐culture with myotubes.
1. The influence of target interaction upon the electrophysiological properties of dissociated ciliary ganglion cells was investigated by testing the sensitivity of the neuronal somal membrane to ionophoretically applied acetylcholine (ACh). Variations in the percentage of cells responsive to the transmitter were measured with time in culture.
G, Crean, G, Pilar, J B, Tuttle, K, Vaca
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Prenatal muscle development in a mouse model for the secondary dystroglycanopathies [PDF]
The defective glycosylation of α-dystroglycan is associated with a group of muscular dystrophies that are collectively referred to as the secondary dystroglycanopathies.
A Leschziner +49 more
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0.15% GAA supplementation enhanced body weight and productive performance in heat‐stressed aged hens. Serum creatine, nitric oxide, and antioxidant capacity increased with GAA, while egg quality remained unaffected. One percent reduced protein diet with GAA maintained performance, whereas 2% reduction caused losses.
Reza Mahdavi +4 more
wiley +1 more source
Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation. [PDF]
Agrin is thought to be the nerve-derived factor that initiates acetylcholine receptor (AChR) clustering at the developing neuromuscularjunction. We have investigated the signaling pathway in mouse C2 myotubes and report that agrin induces a rapid but ...
Deiner, M, Ferns, M, Hall, Z
core

