Results 11 to 20 of about 44,369 (236)

Comprehensive Mapping of Functional Enhancers in Chinese Hamster Ovary Cells. [PDF]

Biotechnol Bioeng
In this study, the authors identified regions with enhancer activity in the CHO cell genome using genome‐wide and targeted STARR‐seq. The analysis revealed thousands of newly identified enhancer sequences, where some of the most active sequences could drive transgene expression at levels similar to or higher than strong viral enhancers.
Santos M, Johari YB, Biggins L, Elliott NC, Schoenfelder S, Boddireddy M, Fabian DK, Anbar M, O'Callaghan PM, Rugg-Gunn PJ.   +9 more
europepmc   +2 more sources

Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions. [PDF]

Biotechnol Bioeng
Proteomic analysis of CHO cells under mild hypothermia (31°C) reveals ER stress responses, ubiquitination dynamics, and adaptive mechanisms. Magnetic immuno‐affinity enrichment and mass spectrometry highlight protein regulation differences in non‐producer versus producer cells, offering insights for optimising biopharmaceutical production of IgG1 and ...
Ryan D, Sideri CK, Henry M, Karuppuchamy S, Efeoglu E, Meleady P.   +5 more
europepmc   +2 more sources

Model-based analysis of N-glycosylation in Chinese hamster ovary cells. [PDF]

PLoS ONE, 2017
The Chinese hamster ovary (CHO) cell is the gold standard for manufacturing of glycosylated recombinant proteins for production of biotherapeutics. The similarity of its glycosylation patterns to the human versions enable the products of this cell line ...
Frederick J Krambeck, Sandra V Bennun, Mikael R Andersen, Michael J Betenbaugh   +3 more
doaj   +1 more source

N-linked glycosylation modulates the immunogenicity of recombinant human factor VIII in hemophilia A mice

Haematologica, 2018
Immune responses to factor VIII remain the greatest complication in the treatment of severe hemophilia A. Recent epidemiological evidence has highlighted that recombinant factor VIII produced in baby hamster kidney cells is more immunogenic than factor ...
Jesse D. Lai, Laura L. Swystun, Dominique Cartier, Kate Nesbitt, Cunjie Zhang, Christine Hough, James W. Dennis, David Lillicrap   +7 more
doaj   +1 more source

Internalization of Ricin in Chinese Hamster Ovary Cells [PDF]

Molecular and Cellular Biology, 1981
Internalization of ricin into Chinese hamster ovary cells has been investigated. Combined treatment with galactose and pronase at 0 degrees C resulted in a complete release of surface-bound [125I]ricin into the media. Galactose-pronase-resistant cell-bound [125I]ricin represents internalized ricin molecules inside the cells. The internalization process
B, Ray, H C, Wu
openaire   +2 more sources

Vessel Ultrasound Sonographic Assessment of Soluble Receptor for Advanced Glycation End Products Efficacy in a Rat Balloon Injury Model

Current Therapeutic Research, 2014
Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem ...
Hyun-Jin Tae, DVM, PhD, Natalia Petrashevskaya, PhD, Ismayil Ahmet, PhD, Sungha Park, MD, PhD, Mark I. Talan, MD, PhD, Edward G. Lakatta, MD, Li Lin, PhD   +6 more
doaj   +1 more source

Expression and functional characterization of His-tagged prestin in Chinese hamster ovary cells [PDF]

PLoS ONE
Ryosei Motoo, Hisashi Sugimoto, Yasunori Donjo, Tomokazu Yoshizaki, Michio Murakoshi   +4 more
doaj   +2 more sources

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Stem Cell Research, 2017
We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector.
Haiyun Pei, Hsu-Yuan Fu, Hiroyuki Hirai, Dong Seong Cho, Timothy D. O'Brien, James Dutton, Catherine M. Verfaillie, Wei-Shou Hu   +7 more
doaj   +1 more source

Metabolism of 25-hydroxycholesterol in mammalian cell cultures. Side-chain scission to pregnenolone in mouse L929 fibroblasts.

Journal of Lipid Research, 1989
Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase.
F R Taylor, A A Kandutsch
doaj   +1 more source

Optimization of human interferon beta protein expression in Chinese hamster ovary cells [PDF]

Journal of Shahrekord University of Medical Sciences, 2021
Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese hamster ...
Nasrin Xodadadi, Alireza Saeidinia, Mehdi Zeinoddini, Rasoul Khalilzadeh   +3 more
doaj   +1 more source