Results 201 to 210 of about 83,358 (225)
Some of the next articles are maybe not open access.
[1] Chinese hamster ovary cells
1987Publisher Summary This chapter describes Chinese hamster ovary (CHO) cells that use for genetic analysis in tissue culture. These cells have been used for the isolation of mutants affecting intermediary metabolism; DNA, RNA, and protein synthesis; membrane functions; and several more complex forms of cell behavior such as cell growth and endocytosis.
openaire +1 more source
Cell Engineering and Cultivation of Chinese Hamster Ovary (CHO) Cells
Current Pharmaceutical Biotechnology, 2010Mammalian cell lines are important host cells for the industrial production of pharmaceutical proteins owing to their capacity for correct folding, assembly and post-translational modification. In particular, Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins.
Takeshi, Omasa +2 more
openaire +2 more sources
[Modification of Chinese hamster ovary cells].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth ...
Da-Zhi, Lai +4 more
openaire +1 more source
Paraquat-resistant cell lines derived from chinese hamster ovary cells
Cell Biology International Reports, 1990Two paraquat-resistant clones, PR-1 and PR-2, were selected from CHO K1 cells pretreated with ethyl methanesulfonate. PR-1 and PR-2, routinely cultured in a normal medium without paraquat, were six fold more resistant to paraquat than the parental CHO K1 cells.
T C, Lee +6 more
openaire +2 more sources
Regulation of hamster α1B-adrenoceptors expressed in Chinese hamster ovary cells
European Journal of Pharmacology, 1996Chinese hamster ovary (CHO) cells were stably transfected to express the hamster alpha 1B-adrenoeceptor, and the function and agonist-induced regulation of the binding properties of these receptors were characterized. The cells expressed approximately 230,000 receptors per cell, with a KD for [3H]prazosin of 140 pM.
S J, Zhu +3 more
openaire +2 more sources
Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells
Biochemical Journal, 2001Phosphatidylglycerophosphate (PGP) synthase catalyses the committed step in the biosynthesis of phosphatidylglycerol and cardiolipin in mammalian cells. Recently we isolated a Chinese hamster ovary (CHO) PGS1 cDNA encoding PGP synthase. In the present study we purified this PGP synthase to near-homogeneity from the mitochondrial fraction of CHO-K1 ...
K, Kawasaki +3 more
openaire +2 more sources
Ultrasound characterization of Chinese hamster ovary cells
The Journal of the Acoustical Society of America, 2012Ultrasonic characteristics of various tissues are currently not known in enough detail to be used reliably for tissue identification or diagnosis. Analysis at a cellular level as opposed to a tissue level can examine these qualities specific to a cell type.
Aditya D. Mahajan +3 more
openaire +1 more source
Histones from Chinese hamster ovary cells
European Journal of Biochemistry, 1983A quantitative analysis of histone variants and of their modified forms in exponentially growing Chinese hamster ovary cells is described. A two‐step analytical procedure has been developed. Total nuclear proteins were first fractionated on a sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis.
G, Joseph +2 more
openaire +2 more sources
Chinese hamster ovary cells sensitivity to localized electrical stresses
Bioelectrochemistry and Bioenergetics, 1999Application of an external electric field on a cell suspension induces an alteration in the membrane structure giving free access to the cell cytoplasm. Under mild pulsation conditions, permeabilization is a reversible process which weakly affects cell viability while drastic electrical conditions lead to cell death.
M C, Vernhes, P A, Cabanes, J, Teissie
openaire +2 more sources
Electrofusion of Chinese hamster ovary cells after ethanol incubation
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1985Electrofusion of Chinese hamster ovary cells is obtained with cells growing in monolayers on a culture dish. The cells were incubated with increasing amounts of ethanol before the field pulsation. The electroperforation was apparently not affected by the incubation but the threshold field intensity required to induce the fusion was shifted to higher ...
G, Orgambide, C, Blangero, J, Teissié
openaire +2 more sources