Results 131 to 140 of about 5,463 (163)

Deacetylation of chitin oligosaccharides of dp 2–4 by chitin deacetylase from Colletotrichum lindemuthianum

Carbohydrate Research, 1997
Chitin oligosaccharides of degree of polymerization 2-4 were deacetylated by purified chitin deacetylase isolated from Colletotrichum lindemuthianum to give their corresponding breakdown products after purification by liquid chromatography. Data from FABMS analyses suggested that N,N',N",N"'-tetraacetylchitotetraose and N,N',N"-triacetylchitotriose ...
K, Tokuyasu, H, Ono, M, Ohnishi-Kameyama, K, Hayashi, Y, Mori   +4 more
openaire   +2 more sources

Chitin Deacetylase from the Plant Pathogen Colletotrichum Lindemuthianum

Plant Science Letters, 1983
Summary Colletotrichum lindemuthianum, the causal agent of anthracnose in beans, produces a chitin deacetylase, most of which is excreted into the medium in shake culture. The enzyme was greatly purified from the culture filtrate and shown to differ significantly from the respective Mucor enzyme; it shows optimal activity at a much higher pH and is ...
H. Kauss, W. Jeblick, D.H. Young
openaire   +1 more source

Kinetic Properties and Role of Bacterial Chitin Deacetylase in the Bioconversion of Chitin to Chitosan

Recent Patents on Biotechnology, 2013
Chitin is an extremely insoluble material with very limited industrial use; however it can be deacetylated to soluble chitosan which has a wide range of applications. The enzymatic deacetylation of various chitin samples was investigated using the bacterial chitin deacetylase (CDA), which was partially purified from Alcaligenes sp.
Ahmed, ElMekawy, Hanaa M, Hegab, Ashraf, El-Baz, Samuel M, Hudson   +3 more
openaire   +2 more sources

Chitin Deacetylase: Characteristic Molecular Features and Functional Aspects

2013
Chitosan has a broad and impressive array of applications in diverse industrial sectors, like pharmaceutics (drug delivery), gene delivery, tissue engineering, food and cosmetics industry, water treatment, and agriculture. To date, majority of the chitosan is produced from thermo-alkaline deacetylation of chitin from crustacean shells.
Nidhi Pareek, V. Vivekanand, R. P. Singh
openaire   +1 more source

Cloning and Expression of Two Chitin Deacetylase Genes ofSaccharomyces cerevisiae

Yeast, 1997
Chitin deacetylase (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin, has been demonstrated in crude extracts from sporulating Saccharomyces cerevisiae. Two S. cerevisiae open reading frames (ORFs), identified by the Yeast Genome Project, have protein sequence homology to a chitin deacetylase from Mucor
C, Mishra, C E, Semino, K J, McCreath, H, de la Vega, B J, Jones, C A, Specht, P W, Robbins   +6 more
openaire   +2 more sources

Chitin deacetylase in cucumber leaves infected by Colletotrichum lagenarium

Physiological and Molecular Plant Pathology, 1990
Abstract Chitin deacetylase assayed by measuring deacetylation of the soluble substrate [3H]-acetyl-glycolchitin, was found to be secreted by Colletotrichum lagenarium growing on an agar medium. The enzyme was first detectable in infected cucumber leaves at the time of appressoria formation and penetration, and the activity increased with the ...
J. Stegrist, H. Kauss
openaire   +1 more source

Chitinase and Deacetylase-Based Chitin-Degrading Bacteria: One-Pot Cascade Bioconversion of Chitin to Chitooligosaccharides

Journal of Agricultural and Food Chemistry
Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to the low catalytic performances of chitin deacetylases (CDAs) and complicated procedures. In this study, a CDA from Arthrobacter sp.
Liwei Qing, Jing Gao, Liuhuan Du, Yiying Liu, Na Guo, Jianan Sun, Hao Dong, Xiangzhao Mao   +7 more
openaire   +2 more sources

A comprehensive account of fungal chitin deacetylases: Aspects and prospects

International Journal of Biological Macromolecules
Chitin deacetylases (CDA, EC 3.5.1.41), found in bacteria, insects and fungi, are the only enzymes in nature that catalyzes the conversion of chitin (polymer of N-acetyl-β-D-glucosamine) to chitosan (polymer of β-D-glucosamine). Presently, the commercial production of chitosan involves using harsh chemicals at high temperatures, causing pollution ...
Namrata, Raghavan, E K, Pathan
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CHITIN DEACETYLASE AND METHOD FOR PRODUCING THE SAME

2003
The invention relates to chitin deacetylase sequences and to proteins coded therefrom, which are suitable for converting biopolymers into glucosamine.
FRAUNHOFER GES FORSCHUNG, ZAKARIA HAYSSAM, OTTO BERND, SCHMALZ CHRISTIAN, SIEFERT EIKE, MEENS JOCHEN, SCHREIBER CHRISTINE, WAGNER-DOEBLER IRENE, BUENGER UTA   +8 more
openaire   +1 more source

Structural basis for directional chitin biosynthesis

Nature, 2022

exaly