Results 161 to 170 of about 2,397 (206)
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Nature New Biology, 1973
CHITIN is a structural polysaccharide composed of β(1→4) linked N-acetyl-D-glucosamine residues, found widely in exoskeletons of animals and in cell walls of green algae and fungi1,2. Chitosan is a partially deacetylated form of chitin2; those commercially available have an average degree of acetylation of 10–30% and are prepared by treating chitin ...
R L, Monaghan +3 more
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CHITIN is a structural polysaccharide composed of β(1→4) linked N-acetyl-D-glucosamine residues, found widely in exoskeletons of animals and in cell walls of green algae and fungi1,2. Chitosan is a partially deacetylated form of chitin2; those commercially available have an average degree of acetylation of 10–30% and are prepared by treating chitin ...
R L, Monaghan +3 more
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Chitosanase activity inBacillus thuringiensis
Folia Microbiologica, 2004The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees
R, Cruz Camarillo +4 more
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Crystallization of a Chitosanase from Streptomyces N174
Journal of Molecular Biology, 1993Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion.
E, Marcotte +4 more
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Journal of Molecular Catalysis B: Enzymatic, 2012
Abstract The bacterial strain OUC1 was found to be closely related to Pseudomonas sp. A-01. A chitosanase gene ( chi ) cloned from the marine bacterium Pseudomonas sp. OUC1 was over-expressed in Yarrowia lipolytica which is generally regarded as safe.
Catherine Madzak +2 more
exaly +3 more sources
Abstract The bacterial strain OUC1 was found to be closely related to Pseudomonas sp. A-01. A chitosanase gene ( chi ) cloned from the marine bacterium Pseudomonas sp. OUC1 was over-expressed in Yarrowia lipolytica which is generally regarded as safe.
Catherine Madzak +2 more
exaly +3 more sources
A Streptomyces chitosanase is active in transgenic tobacco
Plant Cell Reports, 1995Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7 shuttle vector to transform ...
S, El Quakfaoui +3 more
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Chitosanases — properties and applications: A review
Bioresource Technology, 1996Chitosanases which catalyze the hydrolytic degradation of chitosan are to be regarded as important for the extensive carbon and nitrogen recycle which occurs in nature. Chitosanases occur widely in soil microorganisms and in some plants, fulfilling a possible defense role in the latter.
D. Somashekar, Richard Joseph
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Journal of Biochemistry, 2001
We have investigated the mechanism of the interaction of Streptomyces sp. N174 chitosanase with glucosamine hexasaccharide [(GlcN)(6)] by site-directed mutagenesis, thermal unfolding, and (GlcN)(6) digestion experiments, followed by theoretical calculations. From the energy-minimized model of the chitosanase-(GlcN)(6) complex structure (Marcotte et al.,
H, Tremblay +3 more
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We have investigated the mechanism of the interaction of Streptomyces sp. N174 chitosanase with glucosamine hexasaccharide [(GlcN)(6)] by site-directed mutagenesis, thermal unfolding, and (GlcN)(6) digestion experiments, followed by theoretical calculations. From the energy-minimized model of the chitosanase-(GlcN)(6) complex structure (Marcotte et al.,
H, Tremblay +3 more
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Chitosanases in the autolysis of Mucor rouxii
Mycopathologia, 1985The mycelium of Mucor rouxii reached a 50% degree of lysis after 50 days incubation, and was then stable with the incubation time. The pH of the medium was 4.3 when autolysis began, rising to pH 7.6 after 6 days of autolysis and remaining there for the duration of the experiment. Maximum degradation of mycelium occurs during the first days of autolysis.
Fuensanta Reyes +3 more
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Multiple forms of chitosanase activities
Phytochemistry, 1992Abstract Leaf, seed and fruit crude extracts from several species were tested for chitosanase activity using a polyacrylamide gel assay. Chitosanase activity was found in higher and in lower plant species. Depending on extracts, one to six acidic isoforms and one to three basic isoforms could be detected.
Souad El Ouakfaoui, Alain Asselin
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Diversity of chitosanase activity in cucumber
Plant Science, 1992Abstract Chitosanase activity was studied in different organs of cucumber ( Cucumis sativus L.) during development by polyacrylamide gel electrophoretic assays. Some chitosanase forms were present in leaves, roots, flowers, fruits and seeds. Up to five acidic and four basic forms were detected in the various extracts.
Souad El Ouakfaoui, Alain Asselin
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