Results 161 to 170 of about 2,147 (208)
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Purification and characterization of a chitosanase from Serratia marcescens TKU011
Carbohydrate Research, 2008A chitosanase was purified from the culture supernatant of Serratia marcescens TKU011 with shrimp shell wastes as the sole carbon/nitrogen source. Zymogram analysis revealed the presence of chitosanolytic activity corresponding to one protein, which was purified by a combination of ion-exchange and gel-filtration chromatography. The molecular weight of
San-Lang Wang, Tzu-Wen Liang
exaly +3 more sources
Production, purification and characterisation of a chitosanase from Bacillus cereus [PDF]
Research on Chemical Intermediates, 2014 In the present work, a chitosanase was induced from a squid pen powder-containing Bacillus cereus TKU031 medium, and the addition of 0.05 % (w/v) boric acid or sodium tetraborate resulted in 195 and 177 % enhancement, respectively, in TKU031 chitosanase ...
San-Lang Wang +2 more
exaly +2 more sources
Characterization of a new family 75 chitosanase from Aspergillus sp. W-2 [PDF]
International Journal of Biological Macromolecules, 2015 A new chitosanase gene of glycoside hydrolase (GH) family 75, csnw2, was cloned from an isolated strain Aspergillus sp. W-2 (CGMCC7018). The mature CsnW2 protein, fused to His-tag at C-terminus, was expressed in Pichia pastoris X-33 and purified with the
Heng Yin, Yuguang Du
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Insights into promiscuous chitosanases: the known and the unknown
Applied Microbiology and Biotechnology, 2022Chitosanase, a glycoside hydrolase (GH), catalyzes the cleavage of β-1,4-glycosidic bonds in polysaccharides and is widely distributed in nature. Many organisms produce chitosanases, and numerous chitosanases in the GH families have been intensely studied.
Haipeng Su +4 more
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Nature New Biology, 1973
CHITIN is a structural polysaccharide composed of β(1→4) linked N-acetyl-D-glucosamine residues, found widely in exoskeletons of animals and in cell walls of green algae and fungi1,2. Chitosan is a partially deacetylated form of chitin2; those commercially available have an average degree of acetylation of 10–30% and are prepared by treating chitin ...
R L, Monaghan +3 more
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CHITIN is a structural polysaccharide composed of β(1→4) linked N-acetyl-D-glucosamine residues, found widely in exoskeletons of animals and in cell walls of green algae and fungi1,2. Chitosan is a partially deacetylated form of chitin2; those commercially available have an average degree of acetylation of 10–30% and are prepared by treating chitin ...
R L, Monaghan +3 more
openaire +2 more sources
Chitosanase activity inBacillus thuringiensis
Folia Microbiologica, 2004The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees
R, Cruz Camarillo +4 more
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Crystallization of a Chitosanase from Streptomyces N174
Journal of Molecular Biology, 1993Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion.
E, Marcotte +4 more
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A Streptomyces chitosanase is active in transgenic tobacco
Plant Cell Reports, 1995Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7 shuttle vector to transform ...
S, El Quakfaoui +3 more
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Chitosanases — properties and applications: A review
Bioresource Technology, 1996Chitosanases which catalyze the hydrolytic degradation of chitosan are to be regarded as important for the extensive carbon and nitrogen recycle which occurs in nature. Chitosanases occur widely in soil microorganisms and in some plants, fulfilling a possible defense role in the latter.
D. Somashekar, Richard Joseph
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Journal of Biochemistry, 2001
We have investigated the mechanism of the interaction of Streptomyces sp. N174 chitosanase with glucosamine hexasaccharide [(GlcN)(6)] by site-directed mutagenesis, thermal unfolding, and (GlcN)(6) digestion experiments, followed by theoretical calculations. From the energy-minimized model of the chitosanase-(GlcN)(6) complex structure (Marcotte et al.,
H, Tremblay +3 more
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We have investigated the mechanism of the interaction of Streptomyces sp. N174 chitosanase with glucosamine hexasaccharide [(GlcN)(6)] by site-directed mutagenesis, thermal unfolding, and (GlcN)(6) digestion experiments, followed by theoretical calculations. From the energy-minimized model of the chitosanase-(GlcN)(6) complex structure (Marcotte et al.,
H, Tremblay +3 more
openaire +2 more sources