Results 181 to 190 of about 2,147 (208)

Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages

Journal of General Plant Pathology, 2003
In combination with lytic infection by virulent phages, a simple method for monitoring transgenic strains of Enterobacter cloacae was developed in this study. First, 15 strains of E. cloacae were used as indicator bacteria to isolate virulent phages with different host ranges.
Yoshihiro Takikawa, Yuuki Ishii, Keiichi Fujiwara, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Yukio Tosa, Shigeyuki Mayama, Hideyoshi Toyoda   +8 more
openaire   +1 more source

Structural insights into the substrate-binding mechanism for a novel chitosanase

Biochemical Journal, 2014
Chitosanase is able to specifically cleave β-1,4-glycosidic bond linkages in chitosan to produce a chito-oligomer product, which has found a variety of applications in many areas, including functional food and cancer therapy. Although several structures for chitosanase have been determined, the substrate-binding mechanism for this enzyme has not been ...
Qianqian, Lyu, Song, Wang, Wenhua, Xu, Baoqin, Han, Wanshun, Liu, David N M, Jones, Weizhi, Liu   +6 more
openaire   +2 more sources

A note onStreptomyces No. 6, a chitosanase-producing actinomycete

Antonie van Leeuwenhoek, 1992
This communication reports the deposit of Streptomyces No. 6 in the National Collection of Industrial Bacteria, Aberdeen, AB2 1RY, Scotland, UK (Accession number 13037) and gives some references on its use in lysing cell walls of fungi in the Mucorales leading to protoplast formation. Its use in biotechnological fields is also referred to.
openaire   +2 more sources

Purification and hydrolytic action of a chitosanase from Nocardia orientalis

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
Chitosanase from the culture filtrate of Nocardia orientalis was purified to apparent homogeneity by precipitation with ammonium sulfate followed by CM-Sephadex chromatography, biospecific affinity chromatography on a Sepharose CL-4B with immobilized chitotriose and by gel filtration on Sephadex G-75.
K, Sakai, R, Katsumi, A, Isobe, F, Nanjo
openaire   +2 more sources

Characterization of a Chitosanase Isolated from a Commercial Ficin Preparation

Journal of Agricultural and Food Chemistry, 2005
A chitosanolytic enzyme was purified from a commercial ficin preparation by affinity chromatographic removal of cysteine protease on pHMB-Sepharose 4B and cystatin-Sepharose 4B and gel filtration on Superdex 75 HR. The purified enzyme exhibited both chitinase and chitosanase activities, as determined by SDS-PAGE and gel activity staining.
Chui-Liang, Chiang, Ya-Min, Chang, Chen-Tien, Chang, Hsien-Yi, Sung   +3 more
openaire   +2 more sources

Chitosanase-Catalyzed Hydrolysis of 4-Methylumbelliferyl  -Chitotr ioside

Journal of Biochemistry, 1999
4-Methylumbelliferyl beta-chitotrioside [(GlcN)(3)-UMB] was prepared from 4-methylumbelliferyl tri-N-acetyl-beta-chitotrioside [(GlcNAc)(3)-UMB] using chitin deacetylase from Colletotrichum lindemuthianum, and hydrolyzed by chitosanase from Streptomyces sp. N174.
Y, Honda, M, Kirihata, T, Fukamizo, S, Kaneko, K, Tokuyasu, R, Brzezinski   +5 more
openaire   +2 more sources

Chitosanase

1998
Dietmar Schomburg, Dörte Stephan
openaire   +1 more source

Screening of Chitosanase-producing Strain Bacillus decisifrondis and Study on the Optimal Conditions of Chitosanase Production

Journal of Chitin and Chitosan, 2015
Kim Joo Won, Park Jae Kweon
openaire   +1 more source

A Chitosanase mutant from Streptomyces sp. N174 prefers to produce functional chitopentasaccharide

International Journal of Biological Macromolecules, 2020
Meng Ding
exaly  

Purification and characterization of chitosanase from Bacillus cereus D-11

Carbohydrate Polymers, 2008
Woo-Jin Jung, Ro-Dong Park, Wan-Taek Ju   +2 more
exaly