Results 181 to 190 of about 2,397 (206)

Structural insights into the substrate-binding mechanism for a novel chitosanase

Biochemical Journal, 2014
Chitosanase is able to specifically cleave β-1,4-glycosidic bond linkages in chitosan to produce a chito-oligomer product, which has found a variety of applications in many areas, including functional food and cancer therapy. Although several structures for chitosanase have been determined, the substrate-binding mechanism for this enzyme has not been ...
Qianqian, Lyu, Song, Wang, Wenhua, Xu, Baoqin, Han, Wanshun, Liu, David N M, Jones, Weizhi, Liu   +6 more
openaire   +2 more sources

Purification and hydrolytic action of a chitosanase from Nocardia orientalis

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
Chitosanase from the culture filtrate of Nocardia orientalis was purified to apparent homogeneity by precipitation with ammonium sulfate followed by CM-Sephadex chromatography, biospecific affinity chromatography on a Sepharose CL-4B with immobilized chitotriose and by gel filtration on Sephadex G-75.
K, Sakai, R, Katsumi, A, Isobe, F, Nanjo
openaire   +2 more sources

Characterization of a Chitosanase Isolated from a Commercial Ficin Preparation

Journal of Agricultural and Food Chemistry, 2005
A chitosanolytic enzyme was purified from a commercial ficin preparation by affinity chromatographic removal of cysteine protease on pHMB-Sepharose 4B and cystatin-Sepharose 4B and gel filtration on Superdex 75 HR. The purified enzyme exhibited both chitinase and chitosanase activities, as determined by SDS-PAGE and gel activity staining.
Chui-Liang, Chiang, Ya-Min, Chang, Chen-Tien, Chang, Hsien-Yi, Sung   +3 more
openaire   +2 more sources

Chitosanase-Catalyzed Hydrolysis of 4-Methylumbelliferyl  -Chitotr ioside

Journal of Biochemistry, 1999
4-Methylumbelliferyl beta-chitotrioside [(GlcN)(3)-UMB] was prepared from 4-methylumbelliferyl tri-N-acetyl-beta-chitotrioside [(GlcNAc)(3)-UMB] using chitin deacetylase from Colletotrichum lindemuthianum, and hydrolyzed by chitosanase from Streptomyces sp. N174.
Y, Honda, M, Kirihata, T, Fukamizo, S, Kaneko, K, Tokuyasu, R, Brzezinski   +5 more
openaire   +2 more sources

Chitosanase

1998
Dietmar Schomburg, Dörte Stephan
openaire   +1 more source

Screening of Chitosanase-producing Strain Bacillus decisifrondis and Study on the Optimal Conditions of Chitosanase Production

Journal of Chitin and Chitosan, 2015
Kim Joo Won, Park Jae Kweon
openaire   +1 more source

A Chitosanase Mutant From Streptomyces sp. N174 Prefers to Produce Functional Chitopentasaccharide

International Journal of Biological Macromolecules, 2020
Meng Ding
exaly  

Purification and Characterization of Chitosanase From Bacillus cereus D-11

Carbohydrate Polymers, 2008
Woo-Jin Jung, Ro-Dong Park, Wan-Taek Ju
exaly  

Purification and Characterization of Two Types of Chitosanase From Aspergillus sp. CJ22-326

Food Research International, 2005
Xiaobin Yu, Wenshui Xia
exaly  

Chitooligomers Preparation by Chitosanase Produced Under Solid State Fermentation Using Shrimp By-Products as Substrate

Carbohydrate Polymers, 2015
Gaurav Kumar Pal, P V Suresh
exaly