Results 171 to 180 of about 2,397 (206)

Properties of Chitosanase from Bacillus cereus S1

Current Microbiology, 2000
Chitosanase from Bacillus cereus S1 was purified, and the enzymatic properties were investigated. The molecular weight was estimated to 45,000 on SDS-PAGE. Optimum pH was about 6, and stable pH in the incubation at 40 degrees C for 60 min was 6-11. This chitosanase was stable in alkaline side.
M, Kurakake, S, Yo-u, K, Nakagawa, M, Sugihara, T, Komaki   +4 more
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Purification and Characterization of a Chitosanase From Serratia marcescens TKU011

Carbohydrate Research, 2008
A chitosanase was purified from the culture supernatant of Serratia marcescens TKU011 with shrimp shell wastes as the sole carbon/nitrogen source. Zymogram analysis revealed the presence of chitosanolytic activity corresponding to one protein, which was purified by a combination of ion-exchange and gel-filtration chromatography. The molecular weight of
Wang San-lang, Kao-Cheng Liu
exaly   +3 more sources

Chitosanase from Streptomyces griseus

1988
Publisher Summary This chapter describes the assay method and procedure for the purification of chitosanase from Streptomyces griseus. The assay is based on the estimation of amino sugars produced in the hydrolysis of glycol chitosan, a water-soluble derivative of chitosan, by the method of Rondle and Morgan, s using glucosamine as a reference ...
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Uncoupling chitosanase production from chitosan

Bioengineered Bugs, 2011
There is a growing interest in chitosanases as enzymatic tools to hydrolyze chitosan into bioactive forms: low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS). However chitosanases are still expensive and methods of large-scale production of these enzymes are not yet established.
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Chitosanase from Bacillus species

1988
Publisher Summary Several chitosanases have been isolated from some microorganisms, including fungi, bacteria, and actinomycetes. This chapter discusses the assay method and isolation procedure for chitosanase from Bacillus species. The assay is based on the estimation of reducing sugars produced in the hydrolysis of soluble chitosan by a ...
Yasushi Uchida, Akira Ohtakara
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[Advances in the structure and function of chitosanase].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2023
Chitosanases represent a class of glycoside hydrolases with high catalytic activity on chitosan but nearly no activity on chitin. Chitosanases can convert high molecular weight chitosan into functional chitooligosaccharides with low molecular weight. In recent years, remarkable progress has been made in the research on chitosanases.
Jie, Xie, Yubin, Li, Jingwei, Liu, Yan, Gou, Ganggang, Wang   +4 more
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Quantitative fluorometric analysis of plant and microbial chitosanases

Analytical Biochemistry, 1992
A quantitative fluorometric assay for chitosanase activity in bacterial and plant tissues was developed. The assay can be conducted with either finely milled preparations of chitosan in suspension or dissolved chitosan; activity is based on measurements of glucosamine (GlcN) or oligomers of GlcN.
W F, Osswald, R E, McDonald, R P, Niedz, J P, Shapiro, R T, Mayer   +4 more
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Characteristics of chitosanases fromAspergillus fumigatus KB-1

Archives of Pharmacal Research, 2003
Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN.
Tae Kyoung, Eom, Kang Man, Lee
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Rapid detection of chitosanase activity in chitosanase gene-transformed strains of Enterobacter cloacae by lytic infection of specific bacteriophages

Journal of General Plant Pathology, 2003
In combination with lytic infection by virulent phages, a simple method for monitoring transgenic strains of Enterobacter cloacae was developed in this study. First, 15 strains of E. cloacae were used as indicator bacteria to isolate virulent phages with different host ranges.
Yoshihiro Takikawa, Yuuki Ishii, Keiichi Fujiwara, Yoshinori Matsuda, Teruo Nonomura, Koji Kakutani, Yukio Tosa, Shigeyuki Mayama, Hideyoshi Toyoda   +8 more
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A note onStreptomyces No. 6, a chitosanase-producing actinomycete

Antonie van Leeuwenhoek, 1992
This communication reports the deposit of Streptomyces No. 6 in the National Collection of Industrial Bacteria, Aberdeen, AB2 1RY, Scotland, UK (Accession number 13037) and gives some references on its use in lysing cell walls of fungi in the Mucorales leading to protoplast formation. Its use in biotechnological fields is also referred to.
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