Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells [PDF]
, 2018 For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct.
Baldi, Lucia +4 more
core
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells [PDF]
, 2015 Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non ...
Andersen, Mikael Rørdam +9 more
core +1 more source
Advanced Functional Materials, EarlyView.This review highlights how machine learning (ML) algorithms are employed to enhance sensor performance, focusing on gas and physical sensors such as haptic and strain devices. By addressing current bottlenecks and enabling simultaneous improvement of multiple metrics, these approaches pave the way toward next‐generation, real‐world sensor applications.
Kichul Lee +17 more
wiley +1 more source
VirusesA gene delivery system utilizing lentiviral vectors (LVs) requires high transduction efficiency for successful application in human gene therapy. Pseudotyping allows viral tropism to be expanded, widening the usage of LVs.
Bat-Erdene Jargalsaikhan +11 more
doaj +1 more source
Preferential localization of a vesicular monoamine transporter to dense core vesicles in PC12 cells. [PDF]
, 1994 Neurons and endocrine cells have two types of secretory vesicle that undergo regulated exocytosis. Large dense core vesicles (LDCVs) store neural peptides whereas small clear synaptic vesicles store classical neurotransmitters such as acetylcholine ...
Edwards, RH +5 more
core
Pre-stage perfusion and ultra-high seeding cell density in CHO fed-batch culture: a case study for process intensification guided by systems biotechnology [PDF]
, 2020 Lisa Stepper +5 more
openalex +1 more source
Mechanotransducing Organic Electrochemical Diode for Crosstalk‐Inhibited Artificial Skin
Advanced Functional Materials, EarlyView.An innovative approach is presented to a stretchable mechanotransducing diode that unifies rectification and tactile‐sensing functionality. This approach enables to fabricate the diode that maintains a large rectification ratio (5 × 102) at a high operational frequency (100 Hz).
Taeyeong Kim +7 more
wiley +1 more source
Codon Optimization of Stem Cell Factor (SCF) gene and evaluation of this gene expression using vector pBudCE4.1 in Chinese Hamster Ovary cells (CHO) [PDF]
Journal of Fasa University of Medical Sciences, 2017 Background & Objective: Stem cell factor (SCF) is a blood cytokine which can play a significant role in the differentiation of blood precursor cells, survival, proliferation and differentiation of mast cells, and it can also increase the proliferation ...
ehsan heidari +3 more
doaj +1 more source
Apolipoprotein E delivery by peritoneal implantation of encapsulated recombinant cells improves the hyperlipidaemic profile in apoE-deficient mice [PDF]
, 2005 Plasma apolipoprotein E (apoE) is a 34-kDa polymorphic protein which has atheroprotective actions by clearing remnant lipoproteins and sequestering excess cellular cholesterol.
Diakonov, IA +7 more
core
PSD-95 Is Associated with the Postsynaptic Density and Not with the Presynaptic Membrane at Forebrain Synapses [PDF]
, 1996 PSD-95, a prominent protein component of the postsynaptic density (PSD) fraction from rat forebrain, has been localized by light microscopy to dendrites of hippocampal neurons (Cho et al., 1992) and to the presynaptic plexus of cerebellar basket cells ...
Hunt, Carol A. +2 more
core +1 more source